microbe - lab final review

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East Los Angeles College *

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020

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Biology

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Feb 20, 2024

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pdf

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36

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Things in purple- unsure Things in red is unanswered Practical review slides Which tube is called a transport media? C What is tube B used for? Motility, O2 requirement What ingredient is tube A lacking? Agar What is the name of this organism? Schistosoma mansoni (class Trematodes phylum platyhelminth) What is the sexual status of this organism? Dioecious
What category does it belong to? Trematodes (flukes) What is the name of this test? Carbohydrate fermentation - Durham test Which tube indicates a positive result? Tube A Note: Bacteria metabolize sugar which results in acidic waste products. If the tubes in the picture are testing for lactose, which one is the potential pathogen? B What does the pink color indicate? Tube B no carbohydrate fermentation. Note: bacteria metabolize proteins resulting in neutral waste product What Gram reaction is shown here? Gram negative bacteria bacilli What antibiotics would you expect this organism to be resistant to? Penicillin or any cell wall inhibitors - narrow spectrum What morphology is shown in the image above? Rod shape. Bacillus
What is the name of this microbe? Trichomonas vaginalis What disease does this microbe cause? Trichomoniasis / vaginitis What is this microbe’s method of motility? Visible flagella What phylum does it belong to? Archaezoa What method of isolation is this? Spread plate Can you determine a concentration from this method? Yes if you know the dilution factor of the plate culture - serial dilution If there are 132 colonies on this plate and the dilution was 10-6, what was the original concentration? 132 x 10 6 = 1.32x10 8 bacteria per mL
What structure is shown here? Capsule Was this slide heat fixed? No Give a possible genus and species name for this organism? Streptococcus mutans What is the name of this media? Blood agar Green - alpha Yellow - beta n/a - gamma How would you describe this reaction? Alpha hemolysis partial breakdown What is the genus and species name of this organism?
Streptococcus pneumoniae What is the genus name of this organism? Aspergillus What name is given to this spore-bearing structure? Conidia (naked/exposed spore) What is the name of this test? Indole Test - tests for breakdown of tryptophan, tryptophan is amino acid Which tube is a positive result? The pink band positive for breakdown of tryptophan (MV test) What is the reagent for this test? Kovac’s reagent add 4 drops after incubation Is this test part of the IMViC? Yes
amino acid tryptophan broken down Which of the white blood cells in this view is the most common and what is its function? Neutrophil - function is phagocytosis Basophil is the one to the far left - histamine releaser (allergies) Which of the white blood cells in this view is less common and what is its function? Lymphocyte - specific immunity
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