BIOS256 Lab Week 3
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BIOS256 OL, Week 3 Lab
Name: Paola Rodriguez
OL Lab 3: Cellular Respiration: Measuring energy consumption during exercise
Learning Objectives
:
Explain the structural changes of glucose and ATP during glycolysis
Analyze blood glucose and lactic acid concentrations of athletes before and after exercise
Determine electron carrier products of the Krebs cycle
Understand the role of the electron transport chain in generating ATP
Experiment on oxygen consumption in mice at various exercise intensities
Cellular respiration is a multistep process by which the cells create energy in the form of ATP to drive its cellular activities. In this simulation, you will learn the differences between aerobic and anaerobic respiration by studying glucose metabolism. You will explore the three stages of cellular respiration: glycolysis, the Krebs cycle, and the electron transport chain.
In this simulation, you will use a mouse model to understand the cellular respiration of basketball players. The experimental exercise data collected from the mouse model and respirometry will be compared to the blood glucose and lactic acid concentrations of the basketball players throughout the game. You will also, be able to explore the cellular processes that occur within the mitochondria and how it contributes to ATP generation. Are you ready to apply your knowledge on glycolysis, the Kreb’s cycle, and the electron transport chain to help basketball players perform their best during the game?
Part 1: Complete Labster- Cellular Respiration: Measuring energy consumption during exercise
Part 2: Report and Reflection
Purpose: Describe in your own words and in complete sentences, the purpose of this experiment.
The goal of this experiment was to look at athletes' blood sugar and lactic acid levels before and
after a workout. It also helped us understand how a cell, its mitochondria, and its cellular respiration work together. We also looked at the difference between anaerobic and aerobic cellular respiration. We also wanted to understand how glycolysis works, how the Krebs cycle works, and how the electron transport chain works to make ATP. Finally, we looked at how oxygen consumption goes up or down at different levels.
Observations: List 2 observations you have made in this simulation.
Two observations I made during this simulation was that Carbs are the primary source of energy. When you work out too much, your body starts to make too much lactic acid, which is caused by anaerobic breathing.
BIOS256 OL, Week 3 Lab
Name: Paola Rodriguez
Answer all the questions below:
1. Why does lactic acid build up in muscles during exercise? (2 points)
This is the result of the breakdown of glucose for energy by the body. This is achieved through the utilization of oxygen and through physical activity. When the necessary amount of oxygen is not available, the formation of lactic acid occurs. Conversely, when there is insufficient oxygen, lactate is converted into energy.
2. How is glucose stored in skeletal muscles? Why cannot glucose be released from skeletal muscles to maintain blood glues concentration? (2 points)
Skeletal muscles contain glucose, which is stored in the form of glycogen. However, skeletal muscles are unable to release glucose due to a deficiency in glycogen synthase, a process that facilitates the release of glucose. This renders the muscle unable to perform this activity.
3. What is the difference between anaerobic respiration and fermentation?
The process of anaerobes is the production of energy from glucose without the presence of oxygen. The process of fermentation is the utilization of enzymes to obtain energy from carbohydrates.
4. Consumption of certain low fat food that contains high amount of sugar can lead to increased fat deposition in the body. Why? (2 points)
Excess sugar leads to weight gain. It also disrupts lipid and carbohydrate metabolism. This usually means that fats are not able to be broken down as energy. Fat is typically stored because it is considered "excess" due to the inability of the body to function due to high sugar levels.
5. What is the relationship between metabolic rate and body temperature? (2 points)
Metabolic rate results in an increase in body temperature. When cells utilize more oxygen to generate ATP, they expend more heart energy, resulting in an increase in temperature.
6. Reflection: Reflect on at least 2 key concepts you have learned from this simulation.
How can you apply it to the physiological processes in the body?
Cellular respiration takes place when there is oxygen present. When there is no oxygen present,
aerobic respiration takes place. Anaerobic (or anaerobic) respiration occurs when there is no oxygen available. However, this is not the most preferred form of respiration. Glucose is partially
metabolized to lactic acid, rather than being metabolized to water and CO2. As a result, there is less energy available to the body. The effect on the body is the formation of lactic acid, which results from increased respiration and a higher heart rate. Drinking a sports drink helps keep glucose levels high, which helps in the generation of energy.
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Related Questions
asap please help.
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10:16 Fri 3 Mar
8 of 19
Assessment criterion 3.1, 3.2:
Part 1
Using a Table similar to the one below, compare the process of aerobic
respiration with that of anaerobic respiration. You should consider as
part of this comparison:
Please adjust your table to fit the number of points you are making.
Aerobic Respiration
Points to
Consider
The specific
pathways (stages)
involved
The cellular
location(s)
Oxygen
requirements
Net ATP yield
Any additional
points for
comparison that
earning Centre.com, 2022
distancelearningcentre.com
cess to Higher Education Diploma
ology / Medicine
iomolecules and Key Biochemical Principles
raded)
ASSESSMENT TAQ SHEET
you discover from
your research
Part 2
Anaerobic
Respiration
Learning
14
Centre.com
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Assessment
Directions: Read and understand each item and choose the letter of the best answer. Write your answer on a separate sheet of
paper.
1. Which is the final electron acceptor in aerobic respiration?
А.
Pyruvate
C. oxygen
D. water
В.
carbon dioxide
2. In the presence of oxygen, all cells synthesize ATP via the process of glycolysis. Many cells also can metabolize pyruvate if oxygen
is not present, via the process of,
C. aerobic respiration
D. electron transport
А.
Fermentation
В.
Oxidative phosphorylation
3. A cell culture was supplied with radioactively iabeled O2. The cells were monitored. In a few minutes the radioactive oxygen atoms
were present in which of the following compounds:
C. NADH and FADH2
D. ATP
А.
Carbon dioxide
B. Water
4. Majority of the CO2 is released during
C. Citric acid cycle
D. Oxidative phosphorylation
A.
Glycolysis
В.
Electron transport chain
5. Suppose that a new specles of organism has been discovered. Scientists have observed its cells under a…
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Assignment 27.04.2021 / Coursework 2
Homeostatic systems maintain a steady-state set point over time using negative feedback. Which one of the following
statement is NOT TRUE for stability of metabolic system:
Select one:
Oa. A positive feedback system is stable over time, maintaining a steady state.
Ob. Metabolic systems that oscillate must have multiple control variables
Oc. Step changes in a controlled variable will return the system to its original state using negative feedback
O d. Perturbations from steady state, either positive or negative. result in recovery reactions that return the system
to its original state
Next page
Coursework 3-
sIWeek6 EXTRA LECTURE
art 3 19.04.2021
Jump to.
01:09 3
EN
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Preparation: Please review the notes from D2L as well as the presentations provided in the unit plan to complete this assessment.
Visual Representation: Please choose a few diagrams from the presentation, online or D2L that clearly illustrates the processes of cellular respiration. You would need an annotated diagram to explain the processes of cellular respiration which include the following: glycolysis, pyruvate oxidation, krebs cycle, electron transport chain, anaerobic fermentation. Your diagram can be presented in any way that you choose.
Recording: Using your diagram as a reference, please explain each of the processes in your own words using the key vocabulary learned in class. The major concepts have been highlighted in the presentation as well. You can use the screen recording feature, loom, canva and many more digital tools. In your recording, please make connections and relate concepts to real life examples.
Checklist:
Include steps in glycolysis, pyruvate…
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А.
Provide the following information
required about muscle fermentation:
1.
All inputs.
2.
All outputs.
3.
Type of respiration.
4.
Is glycolysis involved?
5.
Is oxygen involved?
Using a comparison
table, compare between stage 2 of cellular
respiration and stage 2 of photosynthesis in
В.
terms of:
1.
Stage name.
2.
All inputs.
3.
All outputs
4.
Specific location inside the
corresponding organelle.
Major event(s) taking place.
5.
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Topic : Connection between Electron Transport & Phosphorylation
Task:
1. Define P/O ratio
2. Explain its importance
State all the explanations in point form
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CELLULAR RESPIRATION AND FERMENTATION
Name:
Date:
Instructor:
Section/Group:
POST-LAB QUESTIONS
1. Both cellular respiration and fermentation ordinarily begin with which molecule?
ves
2. What role does cellular respiration play in the metabolism of an organism?
3. Glucose breakdown results in the breaking of C-H bonds and stored energy is released. Compare
and contrast the end products of fermentation and cellular respiration in terms of their energy
produced.
4. Fermentation results in the net production of only 2 ATP, while cellular respiration results in the
production of at least 36 ATP. Explain these results with reference to the end products of both of
these processes.
Cellular Respiration and Fermentation
93
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C7 Q2:
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Assignment 1
Describe some of the vitamins and minerals that act as
coenzymes in metabolic pathways of carbohydrate, lipid and
protein.
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Please help me with this answer.
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30- Need help with following
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answer all sub-parts.
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Purpose: The purpose of this assignment is to explain how energy is extracted from foods ands used to produce ADT and also describe how the body synthesizes new molecules.
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Please explain questions 3
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Parisa Izadi
sI LAST 2 Assignment 27.04.2021 / Coursework 2
Homeostatic systems maintain a steady-state set point over time using negative feedback. Which one of the following
statement is NOT TRUE for stability of metabolic system:
ut of
Select one:
O a. A positive feedback system is stable over time, maintaining a steady state
uestion
O b. Metabolic systems that oscillate must have multiple control variables
O c. Step changes in a controlled variable will return the system to its original state using negative feedback
O d. Perturbations from steady state, either positive or negative, result in recovery reactions that return the system
to its original state
Next page
Coursework 3-
Biophysics I Week6 EXTRA LECTURE
Part 3 19.04.2021
Jump to...
EN
01:21 3
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IDENTIFICATION
1. A type of reaction that occurs when an energy absorbing reaction requires an
input from an energy releasing reaction.
2. What does ATP stands for?
3. What does ADP stands for?
4. When a phosphate bond is broken energy is released from ATP. This type of
reaction is
5. To reconnect the phosphate with ADP energy must be absorbed from the
breakdown of glucose during cellular metabolism. This type of reaction that occurs
with glucose is
6. A biochemical process that involves the addition of phosphate to an organic
compound.
7. How many kilojoules is released with ADP is reduced to ADP?
8. ATP is also formed from the process of cellular respiration in the mitochondria of a
cell through
9. ATP is also produced in bacteria in the absence of oxygen called
10. A method in food processing which can produce small amounts of ATP is called
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Q26
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please help asap
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answer the following question posted
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B- What does it mean "redox steps" in some steps of the glycolysis? (Less than two
sentences). (8 pts)
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Chapter : Lipid Metabolism"Draw a general pathway for converting carbohydrates to fatty acids in a liver cell, and indicate which processes occur in the cytosol and which occur in motochondria"
Thanks. Please write the answers clearly.
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aspo
brain
A O 3) Matching.
Complete the formula for the Krebs Cycle:
glucose (sugar)+
- carbon dioxide
energy C8
ATP)
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6
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Topic 3: Metabolism
a. Describe anabolism and catabolism. Write a few differences and examples of both. Write at least 2-3 features of each step of respiration metabolism.
b. Describe the process of oxidative phosphorylation and ETC in bacterial cells. Include the roles and features of ETC members
c. Explain how fermentation is different from respiration.
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Question attached as a picture.
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Discuss with reference to the biological role of glucose and oxygen, the process of aerobic cellular respiration. Add details regarding the 4 stages. Glycolysis, link reaction, Krebs cycle, electron transport chain. Also add the significance of the role of oxygen.
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- asap please help.arrow_forward10:16 Fri 3 Mar 8 of 19 Assessment criterion 3.1, 3.2: Part 1 Using a Table similar to the one below, compare the process of aerobic respiration with that of anaerobic respiration. You should consider as part of this comparison: Please adjust your table to fit the number of points you are making. Aerobic Respiration Points to Consider The specific pathways (stages) involved The cellular location(s) Oxygen requirements Net ATP yield Any additional points for comparison that earning Centre.com, 2022 distancelearningcentre.com cess to Higher Education Diploma ology / Medicine iomolecules and Key Biochemical Principles raded) ASSESSMENT TAQ SHEET you discover from your research Part 2 Anaerobic Respiration Learning 14 Centre.comarrow_forwardpls help with this question all partsarrow_forward
- Pls help ASAP.arrow_forwardAssessment Directions: Read and understand each item and choose the letter of the best answer. Write your answer on a separate sheet of paper. 1. Which is the final electron acceptor in aerobic respiration? А. Pyruvate C. oxygen D. water В. carbon dioxide 2. In the presence of oxygen, all cells synthesize ATP via the process of glycolysis. Many cells also can metabolize pyruvate if oxygen is not present, via the process of, C. aerobic respiration D. electron transport А. Fermentation В. Oxidative phosphorylation 3. A cell culture was supplied with radioactively iabeled O2. The cells were monitored. In a few minutes the radioactive oxygen atoms were present in which of the following compounds: C. NADH and FADH2 D. ATP А. Carbon dioxide B. Water 4. Majority of the CO2 is released during C. Citric acid cycle D. Oxidative phosphorylation A. Glycolysis В. Electron transport chain 5. Suppose that a new specles of organism has been discovered. Scientists have observed its cells under a…arrow_forwardAssignment 27.04.2021 / Coursework 2 Homeostatic systems maintain a steady-state set point over time using negative feedback. Which one of the following statement is NOT TRUE for stability of metabolic system: Select one: Oa. A positive feedback system is stable over time, maintaining a steady state. Ob. Metabolic systems that oscillate must have multiple control variables Oc. Step changes in a controlled variable will return the system to its original state using negative feedback O d. Perturbations from steady state, either positive or negative. result in recovery reactions that return the system to its original state Next page Coursework 3- sIWeek6 EXTRA LECTURE art 3 19.04.2021 Jump to. 01:09 3 ENarrow_forward
- Preparation: Please review the notes from D2L as well as the presentations provided in the unit plan to complete this assessment. Visual Representation: Please choose a few diagrams from the presentation, online or D2L that clearly illustrates the processes of cellular respiration. You would need an annotated diagram to explain the processes of cellular respiration which include the following: glycolysis, pyruvate oxidation, krebs cycle, electron transport chain, anaerobic fermentation. Your diagram can be presented in any way that you choose. Recording: Using your diagram as a reference, please explain each of the processes in your own words using the key vocabulary learned in class. The major concepts have been highlighted in the presentation as well. You can use the screen recording feature, loom, canva and many more digital tools. In your recording, please make connections and relate concepts to real life examples. Checklist: Include steps in glycolysis, pyruvate…arrow_forwardА. Provide the following information required about muscle fermentation: 1. All inputs. 2. All outputs. 3. Type of respiration. 4. Is glycolysis involved? 5. Is oxygen involved? Using a comparison table, compare between stage 2 of cellular respiration and stage 2 of photosynthesis in В. terms of: 1. Stage name. 2. All inputs. 3. All outputs 4. Specific location inside the corresponding organelle. Major event(s) taking place. 5.arrow_forwardTopic : Connection between Electron Transport & Phosphorylation Task: 1. Define P/O ratio 2. Explain its importance State all the explanations in point formarrow_forward
- CELLULAR RESPIRATION AND FERMENTATION Name: Date: Instructor: Section/Group: POST-LAB QUESTIONS 1. Both cellular respiration and fermentation ordinarily begin with which molecule? ves 2. What role does cellular respiration play in the metabolism of an organism? 3. Glucose breakdown results in the breaking of C-H bonds and stored energy is released. Compare and contrast the end products of fermentation and cellular respiration in terms of their energy produced. 4. Fermentation results in the net production of only 2 ATP, while cellular respiration results in the production of at least 36 ATP. Explain these results with reference to the end products of both of these processes. Cellular Respiration and Fermentation 93arrow_forwardC7 Q2:arrow_forwardAssignment 1 Describe some of the vitamins and minerals that act as coenzymes in metabolic pathways of carbohydrate, lipid and protein.arrow_forward
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