BIOL-112_Lab 1Microscope & Cells JZ

Please complete resolution and magnification

A. Purpose: Figure 1 B. Materials: Microscope Magazine Slides and cover slips Paper towels Pipette Scissors C. Procedure: 1. Careful carry a microscope to your lab area. Make sure to hold it with one hand under the base and one hand on the arm as shown in Figure 1. 2. Plug the microscope in and turn it on. Take a moment to look at all the parts of the microscope. Then look at your ocular lens. What is the magnification of the ocular lens (eye piece)? Figure 2 3. Fill in the chart to show the total magnification for each objective lens. Magnification of Ocular Lens Magnification of Objective Lens Objective Lens Total Magnification Low Power Medium Power High Power

answer the following: instruction.match the name of the major part (listed below) with its location on the microscope, and give a very brief description of what each is used for:

9:06 LTE 4) Answer true or false to each of the following statements. On high power, you should use the coarse adjustment knob. The diaphragm determines how much light shines on the specimen. The low power objective has a greater magnification than the scanning objective. The fine focus knob visibly moves the stage up and down. Images viewed in the microscope will appear upside down. If a slide is thick, only parts of the specimen may come into focus. The type of microscope you are using is a scanning microscope. For viewing, microscope slides should be placed on the objective. In order to switch from low to high power, you must rotate the revolving nosepiece. The total magnification of a microscope is determined by adding the ocular lens power to the objective lens power.

Fill out the chart

1. Identify features of Dark-field Microscopy (1-2 paragraph) 2. Give three examples of the uses of microscopes outside the field of biology and medicine. (3 examples with 5sentences explanation each)

Saved Label the following parts of this typical light microscope. Mechanical stage Light source Ocular lens Fine focus Primo Star Coarse focus Mechanical stage Revolving nosepieces Conderser Lamp voltage control Lamp voltage control Objective lens Objective lens Conderser Ocular lens Diaphragm Revolving nosepieces Fine focus Coarse focus Diaphragm Light source

QUESTIONS TO ANSWER: I. Basic Microscopy 1. State 3 ways to handle the microscope properly.

Which of the following microscopy techniques can be used in live cell imaging in a live animal. 1. Structured illumination microscopy 2. Stimulated emission depletion microscopy 3. Photoactivated localization microscopy 4. Two-photon excitation microscopy 5. Total internal reflection microscopy 6. Light-sheet microscopy O4 and 6 O2, 3 and 6 O1 and 4 3 and 5 O2 and 4

USING & FOCUSING THE MICROSCOPE: 1. List the following items into their correct order for proper set-up & use of the microscope: set scanning lens (4x), set low power lens (10x), turn on power, place slide on stage, set light to medium, look through eyepiece (= oculars), fine adjustment focus, coarse adjustment focus, center specimen 1. 2. 3. 4. 5. 6. 7. 8. 9.

Direction: Read and analyze the following laboratory experiment and answer the following question. PART 3: PLASMOLYSIS Materials: safety goggles, red onion, dropper, slides & cover slips, tweezers/ forceps, compound microscope, iodine, small knife, water, salt (5% and 10% solution) Methodology: 1. With goggles on, carefully cut the onion into wedge shaped pieces using a knife. 2. Use an eye dropper to place a drop of water in the center of a microscope slide. Use the tweezers to peel a thin layer of skin tissue from the thick part of the onion wedge and place it in the center of the microscope slide. 3. 4. 5. Add a drop of water and a drop of iodine over the onion tissue on the slide. Carefully lower a cover glass slip at an angle on the stained tissue to allow air bubbles to escape. 6. Examine the prepared slide under the compound microscope at 100X magnification. 7. Record what the cells look like. 8. Prepare a 5% salt solution by adding 5 grams of salt (measure with balance) per 100…

The specimen you are examining is very thin and transparent. What can you do to make it easier to see

Part A: Observing a Wet Mount Slide Without using a Microscope color wheel Low Power ( How does A) to the right B) to the left color wheel High Power ( appear to move when you move the slide in the following directions? C) away from you D) toward you Observations: 1. How does the appearance of f change in going from low power to high power? 2. Describe the image of as it compares to the actual letter on the paper. 3. What happens to the field of view when you go from low power to high power?

PART C: CALCULATING THE DIAMETER OF THE FIELD OF VIEW (FOV)_ The field of view (FOV) is the circular area you can see when you look through the microscope. The diameter of the field of view is different depending on which objective lens you are using. For example, you are using the medium-power objective lens, then the area you can see is actually smaller than if you were using the low-power objective lens. Knowing The diameter of the field of view can help you estimate actual size of objects / cells seen through the microscope. When the revolving nose piece is turned to the low power objective lens, a dear plastic ruler can be placed on the microscope stage (see figure 1). Then, the coarse adjustment knob can be used to focus on the millimeter marks of the ruler making sure that one of the milimeter marks is at the left edge of the field of view (see figure 2). NOTE: Slage cip I-1000 Objects in the FOV are usually measured in micrometers (um). To convert, a FOV in mm, times it by 1000…

Give a particular use/uses of the following types of a microscope: 1. Research microscope 2. Stereomicroscope 3. Student microscope 4. Projection microscope

3 6 9 12 V Stage control knob 1. Mechanical support - is used for carrying the microscope Fine adjustment knob 2. Platform upon which specimen is placed Objective 3. Brings object into approximate focus Diaphragm/Iris 4. Eyepiece/ocular 5. Collects light from source and focuses it upon specimen Controls amount of illumination used to view the object; impacts contrast Stage nation Base 6. Lenses you look into 7. Provides more precise focusing on the object 8. Revolving device that holds the Condenser objective lenses 9. Provides additional magnification of Arm varying powers 10. Mechanical support rests on flat Nosepiece Coarse adjustment knob Stage clip surface of table 11. Hold slides in place 12. 122 Provides mechanical means to move the stage

Question:- What is the best superresolution microscopy method to resolve in z direction.

State the function of each part of the compound light microscope.

Please help me understand this question

Write T (True) or F (False) for the following statements. 1. The objective lens is the one nearest to the observer's eye. 2. Only special lens paper should be used to clean the objectives, oculars, and the condenser of the microscope. 3. The working distance is the distance between the specimen and the objective lens. _4. Magnifying a blurred image generally reveals further details. 5. When a microscope is parfocal, you should not have to use the coarse adjustment at higher magnification. _6. The total magnification of the object seen at high dry power is approximately 40X. 7. Immersion oil can be used to increase resolution of all the objective lenses in a brightfield microscope. _8. Always be sure to oil your microscope lenses before returning the instrument to its designated space. 9. Animals will not survive without mitochondria because they will not be able to make ATP. 10. The use of normal saline solution (NSS) in viewing cheek cells is to prevent lysis or crenation of cells.

Question:- Starting with data collection, describe the steps necessary to determine a 3-Dimensional structure using electron microscopy methods.

Analysis: Write your answers on the space provided. 1. How does the letter “e” as seen through the microscope differ from the way an “e” normally appears? 2. When you move the slide to the left, in what direction does the letter “e” appear to move? When you move it to the right? Up? Down? 3. How does the ink appear under the microscope compared to normal view? 4. Why does a specimen placed under the microscope have to be thin?

Shown below are several micrographs (images from microscopes) that were taken using different microscopy techniques. Write down what method of microscopy was used and why you thought so. Choices for microscopy techniques are: Transmitted Light Microscopy (techniques of brightfield, phase-contrast, darkfield, DIC all fall under this), Fluorescence Microscopy, Scanning Electron Microscopy, and Transmission Electron Microscopy.

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please do all the questions

5) Label the parts of the microscope. 4 1 5 7 8 Nikon 10 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. CO

Question shown in picture about microscope calculations

PROCEDURE: 1. Set two pencils down parallel from each other. Make them about 2-3 inches apart as the length of your slides to keep things easy. 2. Stick a long piece of tape over the two pencils and to the table on either side of the pencils to hold the tape tightly between the two pencils like a bridge. 3. Don’t touch the sticky side of the tape or you will ruin the microscope. Drop a small drop of water onto the top of the tape using the pipette or medicine dropper. 4. Make 3-4 lines of tape and add a different-sized drop to each one. This will help determine what size of water droplet produces the biggest magnification. 5. Prepare a rectangular shape of plastic cover. Put a small slice of onion. Slide the rectangular shape of the plastic cover with the small slice of onion under the pieces of tape and observe the size of the onion on different droplets. Write your observations on the table below. No. of drops Observation