Zivistan PhysioEx Exercise 3 Activity 1
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PhysioEx Exercise 3 Activity 1
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PhysioEx Lab Report
Exercise 3: Neurophysiology of Nerve Impulses
Activity 1: The Resting Membrane Potential
Name: Zivistan Fidan
Date: 24 September 2023
Session ID: session-e56709c6-24c6-7c14-3822-b605474d4296
Pre-lab Quiz Results
You scored 100% by answering 4 out of 4 questions correctly.
Experiment Results
Predict Question
Stop & Think Questions
What is the approximate concentration of K
inside a typical cell (intracellular
concentration)?
You correctly answered: 150 mM.
1
+
What is the approximate concentration of K
outside a cell (extracellular concentration)?
You correctly answered: 5 mM.
2
+
What is the approximate concentration of Na
inside a cell (intracellular concentration)?
You correctly answered: 5 mM.
3
+
What is the approximate concentration of Na
outside a cell (extracellular concentration)?
You correctly answered: 150 mM.
4
+
Predict Question: Predict what will happen to the resting membrane potential if the
extracellular K
concentration is increased.
Your answer: The resting membrane potential will become less negative.
1
+
What is the polarity of the resting membrane potential (voltage)?
You correctly answered: negative.
1
What does it mean that the voltage just inside the membrane is negative?
2
9/24/23, 11:08 AM
PhysioEx Exercise 3 Activity 1
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Experiment Data
Extracellular Fluid (ECF)
Microelectrode Position
Voltage (mV)
Control
Cell body, extracellular
0
Control
Cell body, intracellular
-70
Control
Axon, extracellular
0
Control
Axon, intracellular
-70
High K+
Axon, intracellular
-40
High K+
Axon, extracellular
0
High K+
Cell body, extracellular
0
High K+
Cell body, intracellular
-40
Low Na+
Cell body, intracellular
-72
Low Na+
Cell body, extracellular
0
Low Na+
Axon, extracellular
0
Low Na+
Axon, intracellular
-72
You correctly answered: There are more negative charges than positive charges just inside
the membrane.
The membrane of most cells, including neurons, contains passive, open, K
leak channels.
Given the normal K
concentrations and the resultant concentration gradient, which
direction would K
be expected to move (diffuse) through these leak channels?
You correctly answered: out of the cell .
3
+
+
+
What effect does increasing extracellular K
have on the net diffusion of K
out of the cell?
You correctly answered: It decreases the net diffusion of K
.
4
+
+
+
Which way would Na
move across the membrane if there were open Na
channels?
You correctly answered: Na
would diffuse into the cell.
5
+
+
+
The membrane has open K
channels, and changing extracellular K
concentration results in
a change in membrane potential. Changing the extracellular Na
concentration does not
significantly change the membrane potential. What do your results suggest about the
number or state (open or closed) of Na
channels in the resting membrane of a neuron?
You correctly answered: Na
channels are mostly closed.
6
+
+
+
+
+
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Lab 2.1 - Prelab
PhysioEx Ex. 3, Activities 1 and 3
Before coming to class, complete PhysioEx: Exercise 3 (Nerve Impulses in Single Neurons) Activities 1 and 3. Note that
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Activity 1: Resting Membrane Potential (RMP).
The axon membrane contains two kinds of Na* and K* channels. Leak channels are always open to allow a slight influx of
Na+ and larger efflux of K*. This helps generate the resting membrane potential (RMP). These simulations demonstrate
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