Process of meiosis

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University of North Texas *

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Biology

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Oct 30, 2023

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- Sexual reproduction involves the fertilization of two cells from two individual organisms. - The resulting cell contains two sets of chromosomes, also known as diploid cells. - To avoid a continual doubling in the number of chromosome sets in every generation, the diploid cell must reduce its chromosome sets before fertilization can occur again. - This reduction involves a nuclear division called meiosis. - Most animals and plants are diploid, with two sets of chromosomes in each somatic cell. - Homologous chromosomes are matched pairs containing genes for the same traits in identical locations along their length. - Diploid organisms inherit one copy of each homologous chromosome from each parent. - Meiosis reduces the number of chromosome sets from two to one and creates unique recombinant chromosomes. - Meiosis employs many of the same mechanisms as mitosis, but the starting nucleus is always diploid, and the nuclei that result at the end of a meiotic cell division are haploid. - Meiosis II takes place in a way that is similar to mitosis. Interphase - Meiosis is preceded by an interphase consisting of the G1, S, and G2 phases, which are nearly identical to the phases preceding mitosis. - The G1 phase is the first phase of interphase and is focused on cell growth. - In the S phase, the DNA of the chromosomes is replicated, resulting in each chromosome having two identical copies called sister chromatids. - During the G2 phase, the cell undergoes the final preparations for meiosis, including replicating the centrosomes that organize the microtubules of the meiotic spindle. - The first meiotic phase then begins, during which the centromeres holding the sister chromatids together are pulled apart. Meiosis 1 - Early in prophase I, chromosomes are visible microscopically and homologous chromosomes are brought close together by associated proteins.
- The tight pairing of homologous chromosomes is called synapsis, and during this process, crossing over occurs between homologous chromatids. - Crossing over results in recombinant chromosomes that contain a new combination of maternal and paternal alleles. - Chiasmata are the points of contact between homologous chromosomes after crossing over has occurred. - As prophase I progresses, the association between homologous chromosomes breaks down, and the chromosomes continue to condense. - At the end of prophase I, the pairs are held together only at chiasmata and are called tetrads. - Crossover events are the first source of genetic variation produced by meiosis. - A single crossover event between homologous non-sister chromatids leads to a reciprocal exchange of equivalent DNA between a maternal and paternal chromosome, resulting in a recombinant sister chromatid with a new combination of maternal and paternal genes. - Recombinant chromosomes have a mixture of maternal and paternal sequences, while non- recombinant chromosomes are completely maternal or paternal. - The key event in prometaphase I is the attachment of spindle fiber microtubules to kinetochore proteins at centromeres. - In metaphase I, homologous chromosomes are arranged in the center of the cell with kinetochores facing opposite poles, and their orientation is random. - This randomness, called independent assortment, generates the second form of genetic variation in offspring. - The number of variations depends on the number of chromosomes, and there are over eight million possibilities for humans. - To summarize the genetic consequences of meiosis I, maternal and paternal genes are recombined by crossover events during prophase I, and random assortment of tetrads at metaphase produces a unique combination of maternal and paternal chromosomes. - In anaphase I, the spindle fibers pull linked chromosomes apart, breaking chiasma connections. - In telophase I, separated chromosomes arrive at opposite poles, and cytokinesis separates the cell contents. The resulting cells are considered haploid, with only one full set of chromosomes, although sister chromatids are no longer identical due to crossovers. Meiosis II
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