growth media 11:1

.pdf

School

University of Utah *

*We aren’t endorsed by this school

Course

1610

Subject

Biology

Date

Dec 6, 2023

Type

pdf

Pages

3

Uploaded by ProfFlag12641

Report
11/11/23, 9:59 AM OneNote https://uofutah-my.sharepoint.com/personal/u1254641_umail_utah_edu/_layouts/15/Doc.aspx?sourcedoc={53a2fb1c-14d7-492a-a8a4-87ce7258628d}&action=view… 1/3 11/01/2023 Growth on different types of media Wednesday, November 01, 2023 9:14 AM Date: 11/01/2023 Hypothesis: If we streak on different media plates (R2A, LB, 10% TSA and MacC), we could possibly see different levels of growth as well as different colors, as MacConkey is selective and differential. Protocol: 1. Label petri dish on the bottom, not the lid. 2. Obtain sterile toothpick and collect a small bacteria sample from the plate. 3. Streak the four different media plates (R2A, LB, 10% TSA, and MacC) with the isolated colonies that had somewhat a zone of inhibition using the aseptic laboratory technique. 4. Store plates upside down and refrigerate them until the next lab. Results:
11/11/23, 9:59 AM OneNote https://uofutah-my.sharepoint.com/personal/u1254641_umail_utah_edu/_layouts/15/Doc.aspx?sourcedoc={53a2fb1c-14d7-492a-a8a4-87ce7258628d}&action=view… 2/3 We see effective growth on all forms of plates. LB, TSA, R2A all showed strong growth. On the MacC plate, we see a reddish pink plate color, indicating a lactose fermenting colony. The growth on MacC is minimal, but the little growth we see is bright red in color. Conclusion: We conclude that by streaking the isolated colonies with zones of inhibition, we saw different levels of growth depending on the plate. From most growth to least growth, the list includes LB, 10% TSA, R2A, and then MacC. Since we saw growth on the MacC plate, we are able to say that the colony we streaked is gram negative. We also saw color change to a reddish/pink color, which indicates lactose fermentation.
Your preview ends here
Eager to read complete document? Join bartleby learn and gain access to the full version
  • Access to all documents
  • Unlimited textbook solutions
  • 24/7 expert homework help