CW InLab6Assignment_Blood_Lymph_ELISA

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George Washington University *

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Biology

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Dec 6, 2023

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L ABORATORY 6 B LOOD , L YMPHATIC S YSTEM & ELISA A NTIBODY D ETECTION I N -L AB #6 A SSIGNMENT Name: Casey Waggett Anytime you see a grayed-out box that is where you need to type your answers. Once you complete submit this file on our Lab Blackboard site. Use the voiceover ppt videos, procedure videos, your Morton Manual and BSU lab manual to assist in answering the following questions. For the following questions use anatomical terminology found in your BSU Lab Manual or Morton Lab Manual. Blood Analysis Exercises: Complete the following tables as you watch the Blood Cell Count Exercise video on Bb. Do the best you can with cell numbers. Table 1: Blood cell count data Cell Type Cell number Percent of Cells RBC 3 8 7 0.3 % WBC 1 6 2 9.7 % Table 2: White Blood Cell count data for normal and unknown blood slides Control Slide % of total Leukocyte: Unknown Patient Leukocyte Diamete r ( m) Number observed % of total Leukocyte s # observed % of total Leukocytes Neutrophil s 10-12 45 56 3 6.8 Eosinophils 12 3 3.75 4 12.1 Basophils 7 2? 2.5 5 11.4 Lymphocyt es 7-10 25 31.25 30 68.18 Monocytes 15 5 6.25 2 4.5 Total = 100% Total = 100% Blood Review Questions on lab activities 1. The most numerous leukocyte is: neutrophils 2. Examples of granulocytes include the following: monocytes 3. Also called an erythrocyte; anucleate formed element: neutrophil 4. Actively phagocytic leukocytes include: eosinophils and basophils 5. Examples of agranulocytes include: lymphocytes and monocytes
6. Their number rises during parasite infections: eosinophils 7. Releases histamine; promotes inflammation: mast cells 8. Transport oxygen: erythrocytes 9. Increases in numbers during prolonged infections: lymphocytes 10. What is the average life span of a red blood cell? How does its anucleate condition affect its life span? 120 days, when the RBC's ATP reserves have been exhausted, the membrane begins to fragment. Without DNA to direct mRNA synthesis, needed enzymes cannot be made. 11. From memory, attempt to describe the structural characteristics of each of the following blood cell types as accurately as possible, and note the percentage of each in the total white blood cell population. Eosinophils: Eosinophils have large, red-staining cytoplasmic granules; figure 8 or bilobed nuclei; 1-4% of WBCs. Neutrophils: Neutrophils have pale pink cytoplasm with fine granules; nucleus is multilobed and stains deep purple; 40-70% of WBCs. Lymphocytes: are small cells with sparse pale blue cytoplasm and dark purple-staining spherical nucleus; 20-45% of WBCs. Basophils: have sparse dark blue cytoplasmic granules that may obscure the large U-shaped nucleus which stains the dark blue; 0.5% or less of WBCs. Monocytes: have abundant gray-blue cytoplasm, dark blue-purple nuclei (often kidney shaped); 4-8% of WBCs. Fill in the tables below after you watch the Blood Overview PPT Narration video and the Blood Typing video on Bb: Table 1: ABO and Rh System Blood Type Antigens on Erythrocytes Can Receive Blood from: Can Give Blood to: A A antigen O, A A, AB B B antigen O, B B, AB AB A + B antigens O, AB O none O O, A, B, AB
Rh+ D antigen Rh+, Rh- Rh+ Table 2: Agglutination Reactions of ABO Blood-Typing Sera Check box next to + or - Sample A antibodies (anti-A serum) + or - reaction B antibodies (anti-B serum) + or - reaction Rh antibodies (anti-Rh serum) + or - reaction Blood Type Ms. Brown + or - + or - + or - A - Mr. Jones + or - + or - + or - B - Mr. Green + or - + or - + or - A B+ Mr. Smith + or - + or - + or - O - According to the Blood Typing Video: 1. What blood type is Mr. Smith? A- 2. What ABO antigen (agglutinogens) are present, if any? A-, B-, AB+ 3. Is the Rh antigen present on the Mr. Smith? no 4. If this person needed a transfusion, what blood types could this patient safely receive? A- or O- 5. What blood types could safely receive this patient’s blood? A-, A+, AB-, AB+ 6. For the ABO blood group, explain your answer. type AB has both A and B antigens on the surface and neith antibody in the blood plasma. Type O has no antigens on surface and has both antibodies in plasma 7. For the Rh factor, explain your answer. Rh factor is an inherited protein found on surface of red blood cells 8. For the typing experiment shown below, record the blood type for each sample Anti A Anti B BLOOD TYPE: Anti A Anti B BLOOD TYPE: AB B+
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