Amber-Bio 1406

ENZYME CATALYSIS LAB EFFECT OF TEMPERATURE   What chemical reaction is being catalyzed in this experiment? Label the substrate(s), enzyme and product(s).

ENZYME CATALYSIS lab Construct a hypothesis addressing the effect concentration will have on rate of reaction.   What chemical reaction is being catalyzed in the experiment? Label the substrate(s), enzyme and product(s).

9:34 AM You sent       Help me with this one Select true if the statement is CORRECT and false if OTHERWISE   1. Enzymes are catalysts and increase the speed of a chemical reaction without themselves undergoing any permanent chemical change. 2. Catalysis is defined as the acceleration of a chemical reaction 3. if the amount of the enzyme is kept constant and the substrate concentration is then gradually increased, the reaction velocity will decrease. 4. In the Induced-fit Model, if a dissimilar substance which does not fit the site is present, the enzyme rejects it 5. The Michaelis constant Vo is defined as the substrate concentration at 1/2 the maximum velocity. 6. A prosthetic group - an organic substance which is dialyzable and thermostable which is firmly attached to the protein or apoenzyme portion. 7. The rate of an enzyme-catalyzed reaction increases as the temperature is raised beyond optimum temperature. 8. Enzymes can be classified by the kind of chemical…

Result nad Discussion    Lead Acetate Reaction: Samples: lysine, cysteine, methionine Reagents: 10% Sodium Hydroxide (NaOH) and Lead Acetate Pb(CH3COO)2 -To 1 ml of the amino acid solution taken in a test tube, add few drops of sodium hydroxide (40%) and boil the contents for 5-10 mins over a bunsen burner. Cool the contents and add few drops of 10% Lead acetate solution and observe.

Only section 6-31

Pls explain in detail

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[One - Biomolecules] INSTRUCTIONS — Answer the following multiple-choice questions and EXPLAIN in 3-5 sentences why you chose that answer. — Answer properly

Select true if the statement is CORRECT and false if OTHERWISE   1. Enzymes are catalysts and increase the speed of a chemical reaction without themselves undergoing any permanent chemical change. 2. Catalysis is defined as the acceleration of a chemical reaction 3. if the amount of the enzyme is kept constant and the substrate concentration is then gradually increased, the reaction velocity will decrease. 4. In the Induced-fit Model, if a dissimilar substance which does not fit the site is present, the enzyme rejects it 5. The Michaelis constant Vo is defined as the substrate concentration at 1/2 the maximum velocity. 6. A prosthetic group - an organic substance which is dialyzable and thermostable which is firmly attached to the protein or apoenzyme portion. 7. The rate of an enzyme-catalyzed reaction increases as the temperature is raised beyond optimum temperature. 8. Enzymes can be classified by the kind of chemical reaction catalyzed. 9. The living cell is the site of tremendous…

Using this information solve for enzyme activity over time 1 unit - 1 umole per minute.Total reaction volume is 1ml Note: reaction was run for 15 minutes (use relavent formulas) please show full working out. The first part of this question is done 1:5 concentration = 144.7uM and 1:10 concentration 142.4uM. (Use this to solve for enzyme activity) refer to image for all other details and show all working out. Thanks

Biochemistry question: Which of the following statements seen in the attached image about enzyme kinetics is FALSE? Please help with this question. The answer choices are in the attachment. Thank you

Enzyme Concentration 8. Why doesn't it matter if enzymes keep getting added to graph E? What would it take to increase the rate of enzyme activity? Then answer the following questions: 1. Transfer of Energy and Matter: Is an enzyme used up in a chemical reaction? Explain. 2. Transfer of Energy and Matter: How would your diagram be different if the enzyme activity was anabolic instead of catabolic? (E.g., an enzyme to build muscle proteins). a. Rate of Reaction

100 increasing enzyme ativity optimum pH 5 6 9 10 11 7 8 pH 20 40° 60°C Temperature 1. How do pH and temperature affect enzyme activity? 2. What do the graphs suggest about the activity of the enzyme? 3. Draw a graph that represents the role of enzymes in metabolism. (Rubrics on next page.)

8 eBook Select all of the following that could apply to the brown curve. Enzyme concentration is higher. Temperature for an enzyme is too high. Temperature for an enzyme is optimal. Activation energy is lower. Enzyme concentration is lower. Activation energy is higher. Reactants Progress of reaction, Products

Part 1: Assess the following partial results section below by editing it for brevity by omitting any unnecessary parts (1 point), explain why you decided to remove certain sections (1 point): To evaluate inhibitory effects of the selected molecules, 10mM stock solutions of each molecule were prepared in DMSO. A reaction mixture (200μl) was prepared with the same formula optimized for the enzyme activity assay (0.1 M Tris-HCl ph 8, 0.1 M KCI, 25 mM NaCl, 0.25 mM ATP, and two units of inorganic yeast pyrophosphatase) with 10 µM of the sample molecule. The reaction mixture was incubated for 20 minutes at ambient temperature. Enzymatic reaction was triggered by addition of the substrate B (0.2 mM) and the absorbance of the product was monitored at 290 nm for 10 minutes. Six out of 15 sample molecules showed appreciable inhibition at 10 μM (Figure 5). Three of the molecules, A3, A6, and A7 exhibited more than 50% inhibition of the enzyme activity and were further diluted to find the minimal…

Temperature and enzymatic activity Grade 9 students posed the following question: "What is the convenient temperature for the activity of an enzyme?" For this reason, they realized the experiment described below: -They put the same quantity of cooked starch in each of the two test tubes A and B. -They added a small quantity of fresh saliva to each of the two tubes. -Then, they placed both tubes for a convenient duration at different temperatures: •Tube A in ice where the temperature is 0°C Tube B in a water bath where the temperature is 37°C. N.B.: Saliva contains an enzyme: amylase. •The chemical medium is neutral in both tubes A and B. By using an appropriate technique, these students obtained the results shown in the opposite document. 1- Represent, in the same table, the variation of the quantities of cooked starch in the tubes A and B in function of time. 2- Pick out the posed problem by grade 9 students. 3- Analyze the obtained results. What do you conclude? Quantity of cooked…

Chemical Biology, halpp

Sample pH vs Initial Velocity (AA450/60 seconds) a bo16 0.0014 0.0012 o.001 0.0008 0.0006 0.0004 0 0002 pH Sample what does it mean? Does it agree with what we know about enzyme kinetics and why or why nol? If there were anomalies, what were they and what are some possible reasons for this occurring? Was an optimal pH identifiable? Why or why not? Initial Velocity (AA450/60seconds)

Course Code: BIO121 Course Name: Principles of Biology I Read the question carefully first Answer the question-3(b) given chemical reaction according to the question (i) (ii) (iii) with step by step clear explanation. Enzyme X 3 (b) Given a chemical reaction: A + B- (i) (ii) (iii) Enzyme X can be used to catalyze the reaction above. Enzyme X binds to molecule A and B, and converts these molecules into product C. Using the lock and key model, explain how enzyme X is specific to its action. Predict what would happen to the rate of enzyme activity if the concentrations of molecule A and B are increased. Suggest ONE (1) way of how the activity of enzyme X can be controlled.

Give typed full explanation

Need help with number 3

Experiment #1: The Effect of Concentration on Reaction Rate Number of Drops of Enzyme 2 4 6 Reaction Rate (kPa/min) sgorbyt lov 0.27 0.73 1.29 Experiment #2: The Effect of pH on Reaction Rate pH of Solution 4 7 10 Reaction Rate (kPa/min) 0.36 0.97 0.89 Experiment #3: The Effect of Temperature on Reaction Rate Temperature 10°C 35°C 55°C Reaction Rate 18. Predict what would happen if you added 20 drops of enzyme to the test tube. (kPa/min) 0.58 1.43 0.36 17. What type of relationship exists between substrate concentration and reaction rate? Could you explain why you think this relationship exists?

Deepika_Biology  From a kinetics experiment, Kcat was determined to be 55sec^-1. For the kinetic assay, 0.05mL of a 0.05mg/mL solution of the enzyme was used, and the enzyme has a molecular weight of 30,000g/mole. Assume a reaction volume of 3mL. Calculate Vmax (um*min^-1) for the enzyme and catalytic efficiency (in  M^-1sec^-1) for the enzyme. The Km for the enzyme was determined to be 8.3*10^-2M.

3) Catalase Experiment Food Rate of fizzing (1-10) Boiled potato Raw potato Cooked liver 1 Raw liver 10 Students conducted an experiment to check the rate of enzyme action on different types of food. The table shows the results of an experiment in which students placed hydrogen peroxide on several food samples and recorded the relative amount of fizz that each produced. The fizz was produced by oxygen bubbles released by the action of the enzyme catalase, which is found in almost every living cell. The students conducted a second experiment, using the raw chicken liver from the previous experiment. They modified the chicken liver and used: one whole piece, one piece cut into two, a piece that they pulverized in a blender. ALl the Livers were the same mass. The liver was placed in test tubes and hydrogen peroxide was then added to each. Here are the results: Chicken Liver and Enzyme Action Liver Condition Rate of fizzing whole 9 two pieces 11 pulverized 14 Based on the students'…

Enzyme Investigation An enzyme was isolated from digestive juices taken from the small intestine. An experiment was set up to test the ability of the enzyme to break down protein. Two test tubes, labeled A and B, were placed in a hot water bath at 37°C, human body temperature. Test tube A contained only protein and test tube B contained protein and the enzyme. The chart below shows the set-up. After two hours, the contents of both test tubes were analyzed. Test tube A showed only the presence of protein. Test tube B showed the presence of the end products of protein digestion, indicating the enzyme had successfully broken down the protein. Identify the end products of protein digestion that made up the contents of test tube B after the two hours.

This is for question #15.

Answer for the number 3 question, Thank you.

A 45 year-old male presents with fever and cough. He is found to have bacterial pneumonia. He is administered a drug that inhibits bacterial enzyme that catalyzes bacterial DNA synthesis. The drug cited above produced the kinetic data shown below. The mechanism of action of the drug is non-competitive inhibition of enzyme; drug binds to active site. Please explain.

EXPERIMENT 3: Why do enzymes react differently? DATA SHEET Given the following standards and the corresponding standard curve table, what is the amount of substrate consumed given that 1.6 mM of substrate is prepared with an enzyme gave an absorbance of 0.345? 0.4 0.8 1.2 1.6 Substrate with enzyme Concentration (mM) Standard curve table: Varying Absorbance Absorbance Absorbance Final Initial AcP Component Change Depleted umol mM 540 nm 540nm 0.1 0.037 0.088 0.029 0.0784 0.2 0.074 0.022 0.052 0.1406 0.4 0.074 0.074 0.086 0.148 0.2000 0.6 0.222 0.136 0.2325 0.8 0.296 0.204 0.092 0.2487 1.2 0.444 0.345 0.099 0.2676 0.104 0.106 1.6 0.592 0.740 0.488 0.2811 2.0 0.634 0.2865