2023A3SEG23Wadidie
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School
University of Ottawa *
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Course
3356
Subject
Biology
Date
Dec 6, 2023
Type
docx
Pages
2
Uploaded by DoctorUniverse7163
Assignement
1)
Browsing the literature using Pubmed.
Use the
How to use Pubmed
tutorial to answer the following questions:
( /2 Marks)
a)
Recent information: A scientific article on the inhibitory effect of
theaflavin on the RNA polymerase of SARS virus family was published.
Can you give me the Pubmed identifier (PMID) number of the article?
Answer
:
PMID:
35566014
b)
Past information: An article describing the sequencing and cloning of
the SP6 RNA polymerase gene was published a long time ago. Can you
give me the Pubmed identifier (PMID) number of this article?
Answer
:
PMID: 3031606
c)
Specific information: Can you give me the Pubmed identifier (PMID)
number of the article published in 1995 discussing a T7 RNA
polymerase mutant that can used a deoxyribonucleoside
triphosphates as a substrate? Can you tell me the amino acid involved
in this switch of specificity and its position in the protein?
Answer
:
PMID:
7556104
The amino acid is the Tyrosine in Tyr639
d)
Give a reference of a 2022, 2021 or 2020 scientific paper published by
the professor listed for your section ( /0.5 Mark):
Section E:
John Pezacki
Labana P, Dornan MH, Lafrenière M, Czarny TL, Brown ED, Pezacki JP,
Boddy CN. Armeniaspirols inhibit the AAA+ proteases ClpXP and ClpYQ
leading to cell division arrest in Gram-positive bacteria. Cell Chem Biol.
2021 Dec 16;28(12):1703-1715.e11. doi:
10.1016/j.chembiol.2021.07.001. Epub 2021 Jul 21. PMID: 34293284.
2)
Subcloning
Primer design is an essential skill for successful PCR amplification.
Let say that you want to repeat this semester’s subcloning. Using the given
coding sequence, choice of enzymes and vector, (a) design a forward and
reverse primers to amplify the given coding sequence keeping in mind that you
are planning to subclone it into the given vector
( /2 Marks).
(b) Explain how the
forward primer was (or wasn’t) modified to keep the reading frame of the
amplicon in frame with the reading frame of the vector
( /2 Marks).
Coding sequence of your given gene can be found on NCBI. Picture of
the multiple cloning site of the different vectors are available on the
virtual campus of the course (
Hypo vectors
in Lab2 section).
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Related Questions
Implementation
Different stages of Crohn's disease require different treatment. Review the table and determine if the
treatment is recommended for those patients in all stages, those with mild Crohn's disease, those with
moderate Crohn's disease, or those with severe Crohn's disease.
Check the appropriate boxes below.
Low residue diet
Surgery
Corticosteroids
Antibiotics
Biologics
Immunomodulators
Aminosalicylates
All Stages
Mild
Moderate
Severe
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Recommended textbooks for you
- Comprehensive Medical Assisting: Administrative a...NursingISBN:9781305964792Author:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy CorreaPublisher:Cengage Learning
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