Lab 8 Presumptive Testing of Stains - Shruti Tokekar

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Kean University *

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Biology

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Dec 6, 2023

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Lab #8 Presumptive Testing Spermatozoa Comparison A: Unknown Body Fluid Stains: 1. Explain all test procedures under procedures. 2. List Specimen analysis like in a notebook a. Identify the specimen; include source b. Observe & describe any biological fluid stains on the specimen c. Diagram the stains on the specimen i. Indicate all Stains on diagram and number each stain d. Describe the stain analyzed 1. color 2. size 3. texture 4. extent of stain e. List presumptive test performed and the result f. What does this result mean? g. What is the next required serological test to be performed? B: Sperm Slide examination: 1. Observe different species of sperm already mounted on microscopes. 2. Note the magnification of the samples. 3. Diagram just one sperm cell from each species. 4. Describe a. size b. shape (pay particular attention to the head) 5. List species in order according to head size from smallest to largest. Acid Phosphatase (AP) Reagent preparation: STOCK A 0.5 gm O-Diansidine Tetrazotized (Fast Navy Blue Salt) 6.0 gm Sodium Acetate Anhydrous 5 ml Glacial Acetic Acid 50 ml Distilled water STOCK B 0.4 gm ά napthyl acid Phosphate 5 ml Distilled water WORKING REAGENT: 3ml Stock A 5 drops Stock B Bring up to volume to 25 ml.
NAME:__________________________ LAB #8 Presumptive Testing of Stains Objective: Which presumptive test is need for the stain identifies characteristics of the body fluid and is it is positive presumptions for the Prescence of the type of body fluid depending on their test. Complete Test steps Procedures: Kastle-Meyer (KM)Test : detects presence of Blood Procedure: - Moisten the swab with distilled water - Swab the stain with the moisten cotton swab - 1-2 drops ethanol from a pipet - 1-2 drops of phenolphthalein from a pipet - 1-2 drops of 3% hydrogen peroxide Acid Phosphatase (AP) Test : detects presence of Seminal Material Procedure: - Moisten the filter paper with distilled water - Lay on to the entire stain, and press it down with the palm of your hand for 15 seconds - Turn the paper over and pour the AP reagent - 13 seconds and it turns purple Chloride Test : detects the presence of Urine Procedure: - 3 drops of distilled water are added to the stain - 1 drop of 2% nitric acid - 1 drop of silver nitrate - Cloudy reaction Ultra-Violet Light fluorescence : Below describe the type of fluorescence for each fluid: Blood: No Saliva: Yes Seminal Material: Yes Urine: Yes Water: Yes Lab 8 DATA: 1 st Sentence includes: 1. Packaging 2. Identification of Specimen 3. Source 2 nd Sentence includes : Description of specimen, noting all observed Stain(s) by color and location. Next, Diagram the specimen; note each stain indicating each with a stain number.
Next, For each & every Stain……..next to the stain #, list the following: 1. Characteristics of stain, 2. Test performed with the test result What the test result means; What the next required serological test is. Spec #1 is Small brown paper bag contains white paper towel from the kitchen(sc). It’s folded, with two reddish brown stains detected. Mirror image stain when folded. Spec#2 is Small manila envelope containing a white filter paper with dry secretion(v). It’s a fain light yellow stain.
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