Group Assignment #1 Fall 23

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Kennesaw State University *

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1107L

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Biology

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Dec 6, 2023

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docx

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Foundations of Microbiology BIOL 2260 Class Activity #1 in Group in prep for Exam 1 Date 9/7/2023 4 points Peer to Peer Interactions Group 1 Group Leader- Members- 1. Explain Differential versus selective media Q N As 1. Which of the following are true of Bacteria and Archaea? a. Both bacteria and archaea contain extreme halophiles and extreme thermophiles b. Both are known to cause human disease c. Both do not have an organized nucleus d. Both are characterized as prokaryotes 2. All living organism can be classified into three domains: a. Eukaryotes, Prokaryotes, and Viruses b. Bacteria, Archaea, and Eukarya c. Animalia, Plantae, and Microbes d. Bacteria, Fungi, and Protozoa 3. Which of the following is true of scientific names assigned to living microorganisms? a. The scientific name is always underlined or italicized b. The entire scientific name is capitalized c. The scientific name begins with the kingdom name d. The scientific name ends with the genus Group 2 Group Leader- Members-

Explain Positive versus negative staining & Simple versus differential staining Positive dyes the cell, negative dyes the background Q N As 4. Which of the following types of microorganisms and its description are NOT correctly matched? a. Bacteria – prokaryotic b. Fungi – eukaryotic c. Protozoa – small animals d. Helminths – multicellular animals 5. Which of the following are properties true to both bacteria and viruses? a. Both contain DNA and/or RNA b. Both can be visualized by brightfield microscopy c. Both have to replicate inside host cells d. Both have cell walls composed of peptidoglycan 6. A new microorganism has been isolated from hot springs in Yellowstone National Park. It consists of single cells, which appear to lack a nucleus. Chemical analysis shows the presence of both DNA and RNA in the cytoplasm and peptidoglycan in the cell wall. In which of the following groups will this organism be classified? a. Plants b. Fungi c. Archaea d. Bacteria Group 3 Group Leader- Members- Differentiate between Ocular and Objective lens & Define Resolution Objective is the real image and ocular is the virtual image. 7. What structure does light pass through after leaving the condenser in a compound light microscope? A) ocular lens B) objective lens C) specimen D) illuminator 8. Which of the following places the steps of the Gram stain in the correct order? 1-Alcohol decolorizer 2-Crystal violet 3-Safranin 4-Iodine A) 1-2-3-4 B) 2-1-4-3 C) 2-4-1-3

D) 4-3-2-1 E) 1-3-2-4 9. Which of the following is NOT true regarding the acid-fast stain? A) It is used to identify members of the genus Mycobacterium . B) Acid-fast cells retain the primary dye after treatment with acid-alcohol. C) If cells are acid-fast, they are gram-negative. D) Acid-fast cells appear red in a completed acid-fast stain. E) Non-acid-fast microbes appear blue in a completed acid-fast stain. Group 4 Group Leader- Members- Short notes on recombinant DNA technology & give 1-2 examples of Biotech products. Alcohol and cheese production and GMOs 10. The purpose of a mordant in the Gram stain is to A) remove the simple stain. B) make the bacterial cells larger. C) make the flagella visible. D) prevent the crystal violet from leaving the cells. E) make gram-negative cells visible. 11. Which microscope is used to see internal structures of cells in a natural state? A) compound light microscope B) phase-contrast microscope C) darkfield microscope D) fluorescence microscope 12. What is the total magnification of a specimen viewed with a 10x ocular lens and a 45x objective lens? A) 4.5x B) 10x C) 45x D) 100x E) 450x Group 5 Group Leader- Members- Write the work done by each of these scientists Louis Pasteur. Robert Koch. Antonie van Leeuwenhoek

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