Problem Set #3_Diffusion and Experimental Design_In Class_BIOL 30_KY
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BIOL 30 Diffusion & Experimental Design In-Class Problem Set #3 1 Group Member 1 Name: Group Member 1 Student ID#: Group Member 2 Name: Group Member 2 Student ID#: Group Member 3 Name: Group Member 3 Student ID#: Group Member 4 Name: Group Member 4 Student ID#: Directions: Your group of 3 or 4 students will have time in class to fill out the answers to the questions. You may use your notes, lecture slides, and textbook to answer the questions. This is to be completed by your group alone and not in coordination with other groups. If you are confused by the problems, please raise your hand, and ask the instructor or SI Leader for help. All group members must be in class to participate in the assignment. If you are found to have put the name of a student that did not participate in the activity, all students will receive a zero for the assignment and you will not be able to drop that grade as your lowest grade when your final In-
Class Problem Set grade. You will receive at least 70% on the assignment if you have put in a good faith effort to complete the problem set. A subset of the problems will be graded to determine your final grade. We will review the answers at the end of class. 1)
If spherical cell radius is halved, which will show the largest proportional decrease: surface area or volume? Why? Hint: You should look up the formulas to calculate the surface area and volume of a sphere. (6 pts) 2)
Diatoms are photosynthetic plankton in the oceans that live inside silicon shells they produce. The shells come in a wide range of shapes and the cells fill their containers. They all tend to be very flat instead of spherical. Why do you think they are flat instead of spherical? (6 pts) a.
To maximize volume b.
To maximize surface area c.
To decrease diffusion distance d.
B & C 3)
Which solution do you expect to have the LONGEST distance between molecules? (6 pts) a.
1 mM b. 10 mM c. 100 mM d. all will have same distance 4)
A typical human cell is 10 μm long. Using the figure below, determine how long it takes GFP to diffuse in water the length of a human cell. Note: 10
1
is 10 μm. (6 pts)
BIOL 30 Diffusion & Experimental Design In-Class Problem Set #3 2 5)
It takes 0.04s for the GFP protein to travel the length of an E. coli
cell (2 μm) in water. Using your answer to the previous question, how many times longer does it take GFP to travel across a human cell (10 μm) as compared to an E. coli
cell in water? (6 pts) 6)
Using the table above, if it takes GFP 2 seconds to travel the length of a very large cell in water, how long will it take GFP to travel that distance in the cytoplasm? (6 pts) 7)
If it takes GFP 0.4 seconds to travel the length of an E. coli cell (2 μm) in cytoplasm
, how long will it take GFP to travel that distance if it is connected to a membrane protein
? Use the table above to help answer this question. (6 pts) The first step in the signaling transduction pathway is the binding of epinephrine to its G protein-coupled receptor. Synthetic agonists can bind to the receptor and mimic epinephrine signaling while antagonists bind and block the effects of epinephrine. In the experiment to the right, an animal is injected with either epinephrine alone or Drug X, which researchers think could be an agonist to the epinephrine receptor. Both epinephrine and Drug X are mixed in sterile saline. The amount of glucose released from the liver is measured to determine the amount of signal transduction that has taken place. 8)
In the graph on the right, what is the dependent variable? (6 pts) 9)
Which type of control is missing in the above experiments, positive control or negative control? What would you use as the missing control? (12pts) 10)
If Drug X is an agonist, do you expect the response to injecting it to be similar to the epinephrine response or the negative control response? (12 pts) Drug X
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