BMEG 310 Homework 4

Subject : Environmental Microbiology  Can u use the information given below to answer these 2 question  1.Provide an aim for this lab 2. Provide objectives  DISCUSSION QUESTIONS   What is the relationship between the resolution power and the useful magnification that may be obtained with the light microscope?  What determines the resolving power of the lens system?                                What is the limit of resolution obtainable with the light microscope?                                                 How you will distinguish between bright field and dark-field microscopy and provide a specific example where each would be method of choice for observing a culture of bacteria?    What advantages does electron microscopy have over light microscopy?    What are disadvantages of electron microscopy over light microscopy?                               #Compare the use and the methodology of TEM with SEM? Provide at least one example where each would be the method of choic

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Practice problems: Do on folder paper. 1. You are observing a cell; total magnification 100X. It looks like about 7 cells will fit across the field of view's diameter. How large is this cell? Explain. 2. For the 2nd specimen, you are using a total magnification of 400X. It's harder to estimate this time, so you approximated that 12 cells would fit across the radius. Explain how you would find out the size of one cell.

Depth of focus  1. Which colored thread comes into focus first, second and third (as observed under 10X)?    2. As observed under 40X and 4 mm objectives?

mead. Post Lab #2 Smear & Stain Preparation Name: Leidiawa MontaNo Date: 1. Why are thick or dense smears less likely to provide a good smear preparation for microscopic evaluation? Please explain. Becapse, it will diminish the amount ds latcan Pass through by mam 1t difficult to See under the micioscol e, s less liket to Prowde a go image. 2. What could potentially happen if you leave the slide exposed for too long to the open flame? Why do you have to be careful? we can form ring Patterns if we expose the slide for ta0 the flame 3. During the preparation of a smear leading into simple staining of the bacterial culture S. epidermidis you forgot to heat fix the slide. What would you see on this slide as compared to a slide that was properly prepared? Please explain. 4. You partner stained bacterial cells and saw only the background and not the actual cell was stained. Your partner thought this was a mistake. Please explain what type of staining method this is, how it works and why the…

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Show all work including equations. Patient is treated AP/PA parallel-opposed fields. The fields are set up isocentrically on a 6 MV Linac, The patient's AP/PA separation is 25cm, and the field size at midplane is 14 x 26 cm (W x L). Calculate the AP field size on the skin.

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TRUE or FALSE only! 1. Problem: Calibrating ocular micrometer using the stage micrometer under the low power objective. Given: OD=27 SD=8 Each space on the stage micrometer=0.0001 inch Based on the given values, the ODV is equal to 0.753 2. Problem: Calibrating ocular micrometer using the stage micrometer under the low power objective. Given: OD=27 SD=8 Each space on the stage micrometer=0.0001 inch Based on the given values, the SDV is equal to 3.54 3. Inoculation chambers can be used in transferring bacterial culture while laminar flow cabinet can be used as permanent storage for biological samples.

Question:- Starting with data collection, describe the steps necessary to determine a 3-Dimensional structure using electron microscopy methods.

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Question: Assume two Silver/Silver-Chloride electrodes are interfacing with human tissue using the Direct contact and Gel-filled contact approaches. In one paragraph, describe which method is the better technique to reduce motion artifacts and explain why.

MATERIAL METHOD RESULT SUMMARY     Lecia DCM3D confocal Microscope 1) Straumann (Straumann AG, Waldenburg, Switzer- land) 2.2mm in diameter (for short, A) (2) Nobel Biocare (Nobel Biocare AB, Goteborg, Sweden) 2.0mm in diameter (for short, B) Xive Implant System (Friadent GmbH, Mannheim, Germany) 2.0mm in diameter (for short, C) (4) Global D (French) 2.5mm in diameter (for short, D) (5) Sweden & Martina (Padova, Italy) 2.5mm in diameter (for short, E)   The microstructural parameters used by the discriminatory analysis revealed many differences between the five manufactures in terms of roughness of the drilling surface. Marenzi et al (2018) have considered that Surface micromorphology, which affects the contact between the drill and bone, has been shown to contribute to heat, wear, and corrosion phenomena due to material abrasion and corrosion resistance through several surface texture indicators Can be seen as a factor. Not only the design, but also the…

question: Can you summarize and explain for me what you want to tell in the article below? Can you explain the figure?  When I read it myself, I do not understand exactly what is meant by the article. It would be nice if you could highlight the important points. You can use them in a figure or diagram to explain. thank you and hava a nice day :) Article:  Nanotechnology Tools to Detect SARS-CoV-2 Standard procedures for detecting the virus from nasopharyngeal and/or oropharyngeal swabs have been reviewed recently and are primarily based on reverse transcription polymerase chain reaction (RT-PCR). Here, we would like to mention some preliminary ideas on nanotechnology-based assays to monitor the presence of SARS-CoV-2. A simplified test and variants thereof to detect viral proteins (e.g., HIV or influenza virus) without the need for expensive equipment is based on the color change of Au NPs bound to antibodies. Similar to the enzyme-linked immunosorbent assay (ELISA) antibodies coupled…

Please complete resolution and magnification

Problem 1: At a total magnification of 100x, a student measures 16.4 ocular micrometer divisions per millimeter. What is the distance, in micrometers, per ocular unit at 100x? Problem 2: At a total magnification of 400x, a student measures 4.1 ocular micrometer divisions per millimeter. What is the distance, in micrometers, per ocular unit at 400x? Problem 3: Notice the pattern in the above 2 problems. What do you think the distance in micrometers per ocular unit at 1000x would be?

Case Study: Lab Errors

Question:- We are using in class 4.5 mm and 40X. a) How big is a specimen that takes up 1/2 the field view at 90X?

ES Practice, Question 07 Select ALL the correctly described types of microscopy: Bright field commonest microscopy using visible light O Dark field/ uses UV light and spotlights specimens O Nomarski/ uses dual beam visible light with high resolution but shallow depth of field O Phase contrast/ dual beam light, contrast comes from phase changes within the specimen 1 2 13 14 15 O Fluorescence/ uses gamma rays to resolve specimen detail 16 17 18 19 20 Study MAY 24 MacBook Pro 80 000 O O O O O

Tighnari is doing preliminary protein studies on redcrest, a vibrant crimson fruit found in the hot deserts of Sumeru. He extracted proteins in the given sample using phosphate buffer. The resulting crude protein extract was subjected to gel filtration chromatography and SDS-PAGE. GFC results: - Absorbance Peak A B Figure 1. Elution profiles of the mixture of protein standards (black) and crude protein extract (blue). Table 1. Components of the calibration mixture and their corresponding molecular weight. U خلفة X A Elution Volume, mL Identity B Bovine Serum Albumin B-lactoglobulinn Horse Myoglobin Crude Protein Extract Molecular Weight, Da 66000 36800 17600