BIO101_Lab 9 Worksheets_Mitosis and Meiosis_Spring 2022
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BIO 101 Lab 09: Mitosis and Meiosis v.2021 63 BIO 101 Lab 09: Mitosis and Meiosis Objectives: •
Name the phases of mitosis and meiosis and describe their characteristics •
Identify the phases of mitosis as viewed through the compound microscope •
Explain the relationship between mitosis and the process of regeneration •
Compare meiosis I with meiosis II in terms of the position of the chromosomes in each stage, changes in chromosome number, and number of daughter cells produced •
Explain the process and importance of crossing-over between homologous chromosomes •
Compare the processes and end products of mitotic and meiotic cell division Background: Mitosis Many of us know that the outer layer of skin is subject to constant wear. To keep this protective layer intact, skin cells must be replaced throughout our lives. Cells of the epidermis, for example, are replaced every 25-45 days. Growth is another activity for which additional body cells are required. A zygote
(fertilized egg) begins life as a single cell that multiplies into many cells as the embryo develops. During childhood, cell multiplication provides the many cells needed for growing into an adult. The type of cell division that makes growth and repair possible is called mitosis
. All new cells are formed by the division of pre-existing cells. A key part of cell division is the replication of DNA in the parent cell
and the distribution of DNA to the daughter cells
. A cell is genetically “programmed” to carry out its function in the body. These instructions are found on the chromosomes
. The normal number of chromosomes in a cell is referred to as the diploid
number (abbreviated 2n
). Each type of organism has a characteristic number of chromosomes. The diploid number of chromosomes in human body cells, for example, is 46. The diploid number in cabbage is 18, in crayfish it’s 200, and in dogs it’s 78. After successful completion of cell division, each daughter cell must also be 2n
as the parent cell was. To achieve this, the dividing cell passes through several recognizable stages during its life span. These stages constitute the cell cycle. During these stages, the dividing cells must solve several problems: •
Each new cell needs a full set of chromosomes. •
This is accomplished during Interphase
of the cell cycle when chromosomes are duplicated during the S phase
. •
During DNA replication, DNA is in a long thin uncoiled state bound to proteins. In this uncoiled state, DNA is referred to as chromatin
. •
After DNA has been duplicated, the long threadlike chromatin coil and fold into compact structures. These condensed chromosomes are visible with a compound microscope. •
These duplicated chromosomes are referred to as sister chromatids
. A cell preparing to divide will contain two complete sets of sister chromatids (one set for delivery into each of the two new daughter cells).
BIO 101 Lab 09: Mitosis and Meiosis v.2021 64 •
Sister chromatids must be attached together. •
A structure called a centromere
fastens the duplicates together. •
This attachment makes it easier to keep track of the sister chromatids when they are sorted into two sets for delivery. •
The pairs of sister chromatids must be pulled apart and delivered to each new cell. •
A structure called the mitotic spindle
hooks on to each pair of sister chromatids at the centromere. •
Spindle fibers
pull the chromatids apart and deliver one chromatid from each pair to each new cell. •
The two cells must be separated into two daughter cells, each with its own set of chromosomes once the sorting and delivery process is complete. •
The process known as cytokinesis
separates the cytoplasm into two halves. •
During cytokinesis in animal cells, the cell membrane pinches into a groove called the cleavage furrow
. In plant cells, the presence of the cell wall causes the formation of a cell plate
instead of a cleavage furrow. During this mitosis exercise, you will identify the various stages (Interphase, Prophase, Metaphase, Anaphase, and Telophase) of cell division. The following figure can be used as a guideline. Meiosis In contrast to mitosis, meiotic cell division
consists of two
nuclear divisions. This special type of cell division happens in the testes and ovaries of animals to allow the organisms to reproduce sexually. As you probably know, half of your genetic information (23 chromosomes) is inherited from your mother and the other half (23 chromosomes) from your father. To generate your diploid
BIO 101 Lab 09: Mitosis and Meiosis v.2021 65 number (
2n
) of 46 chromosomes, the sperm and egg (
gametes
) must contain half the number of chromosomes, and therefore are considered haploid
(
n
). When the diploid zygote is formed, the genetic information will determine all of the physical and physiological traits of the offspring. Meiosis
is the process that generates a haploid gamete from a diploid germ cell
. The stages of meiosis are similar to those of mitosis. The important differences include the following: •
The chromosome number is reduced from diploid to haploid to form gametes. •
An exchange of genetic material takes place during the process called crossing over
. This results in genetically different gametes. •
Meiosis involves two cell divisions (
meiosis I
and meiosis II
). Genes
(
traits
) on a chromosome are often expressed in different forms called alleles
. Individuals inherit two alleles for each trait, one from the mother’s egg and the other from the father’s sperm. These alleles from mom and dad are found on homologous chromosomes
. Homologous chromosomes carry alleles for the same traits (face shape, eye size, etc.), although the genetic information is not identical. The combination of mom and dad’s alleles determines an individual’s genotype
. If the two inherited alleles are different (Bb), an individual is considered heterozygous
for that trait. The allele that is expressed in heterozygous individuals is referred to as dominant
and is represented by a capital letter (B). The allele not expressed in heterozygous individuals is considered recessive
and is represented by a lowercase letter (b). An individual with two identical alleles (BB or bb) is said to be homozygous
for that trait. The physical manifestation of the combination of alleles is called the phenotype
and is what we sometimes outwardly see. Sometimes traits have two possible phenotypes and other times there are three possible phenotypes. The traits with three possible phenotypes result from a different mode of inheritance called incomplete dominance
. This type of inheritance occurs when an allele exerts only partial dominance over the other allele. This results in a blended intermediate phenotype in heterozygous individuals. In this exercise, you will simulate the sexual reproduction of jack jumper ants
. To do this, you will work through the process of meiosis to form female gametes, decode the genetic information carried by these gametes, simulate fertilization, and use the genotype of a fertilized egg to predict the phenotype of the ant offspring. Jack jumper ants (
Myrmecia croslandi
) are native to Eastern Australia and are notable because they have the fewest possible chromosomes for a diploid organism, with their diploid number being 2 and their haploid number being 1. Another interesting feature is that only female ants perform meiosis to produce gametes. Male ants instead produce gametes using mitosis. This means that the processes of homologous chromosome pairing
and crossing over will only take place in female ants. Additional information about jack jumper ants can be found at: https://www.antwiki.org/wiki/Myrmecia_croslandi Jack jumper ants have a number of traits that vary in their population. While some of this variation has been observed and studied, most of the genotypes and phenotypes we will “observe” in this laboratory activity have been invented for the purposes of this activity and should not be taken as actual facts.
BIO 101 Lab 09: Mitosis and Meiosis v.2021 66 The figure below shows a jack jumper ant with several of the invented traits indicated. For the purposes of this lab activity: The head size is either Large
, Medium
, or Small
The body is either Hairy
or Not Hairy
The eye color is either Black
or White
The eye shape is either Oval
or Round
The Antenna length is either Long
, Medium
, or Short
The body color is either Black
, Brown
, or Red
The head color is either Gold
or Black
The leg color is either Orange
or Black
The ant either Does
produce venom or Does Not
produce venom Materials: •
Compound microscope •
Prepared slides of cells in various stages of mitosis •
Planaria •
Ice •
Petri dish with cover •
Razor blade •
Colored beads •
Plastic bag •
Bead connectors •
Dice •
Dissecting microscope
BIO 101 Lab 09: Mitosis and Meiosis v.2021 67 Safety: Follow all standard laboratory safety procedures. Handle the razor blade with care. Procedure: Mitosis Part 1
: 1. Use the mitosis figure in the Background section of this lab to answer the associated questions on the answer sheet. Part 2
: 2. Retrieve a compound microscope from the storage cabinet. 3. Obtain a prepared slide of mitotic cells and carefully secure it on the stage of the microscope using the metal stage clip. 4. Using the 4X scanning lens, find a cell in one phase of mitosis. 5. Increase the power to 40X and draw what you see as it appears under the microscope on your answer sheet. 6. Repeat steps 4 and 5 until all mitotic phases have been identified. Meiosis 1. Obtain a plastic bag from the supply bench. 2. To simulate the process of meiosis in jack jumper ants, you would perform the following steps: a. Obtain a plastic bag from the supply bench. b. Fill your bag with four beads from each jar. Note that there are two jars of beads of each color---striped and solid. The solid-colored beads represent dominant alleles and the striped beads represent recessive alleles for the same trait. c. Get two red centromeres and two yellow centromeres (bead connectors). d. Return to your lab bench and begin your experiment. 3. Determine if your imaginary organism will be male or female by randomly selecting a bead from your bag. Without looking in the bag, remove one bead from the bag. If the bead is solid-color, your imaginary organism is female, if the bead is striped, your imaginary organism is male. Record the sex of your imaginary organism and then place the bead you selected back into the bag.
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Related Questions
A. Interpretation of Graph/Table
Use graph key as guide
A cell mitosis experiment in which the cells count is determined
hourly over a 24 hour period is shown below:
Growth curve:
What conclusions can you draw about the process of mitosis
over a 24-hour period?
Lines: Individual
Continuous Options
Number of Cells.Hour
Courtesy: www.inspiration.com
1. What conclusions can you draw about the process of cell
mitosis over a 24-hour period;
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Help.
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BIO 1331: Organismal Biology, Meiosis In-Class Assignment
8. What is the ploidy of this cell, now?
Name:
Figure 3
9. What happened to the cell between Figure 2 and 3?
10. Do individuals that are homozygous at a given locus have different gamete
genotypes?
11. How many different types of gametes can an individual with the genotypes given
below produce? (Assume no crossing over). What are those gamete genotypes?
a. Aa: Example - this is one gene and the individual is heterozygous, so
they will make two types of gametes: "A" and "a"
b. AaBb
c. AaBbCC
d. AaBbCCDd
What are the possible offspring from a cross of the following two individual parents:
AABbCc x AABbCC; Make a punnett square for each gene individually (A, B & C)
"A"
"B"
"C"
12. List the possible genotypes of the offspring for the three genes (A, B and C)
combined:
2
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GQ5
arrow_forward
Attempt all questions.
la) with examples, distinguish between.
Continuous and discontinuous variation.
ii) Dominance and co dominance.
iii) Dominant and recessive gene.
iv) Chromosome and a gene.
i)Centromere and chiasmata.
5 Describe what happens at the following stages of mitosis.
Prophase.
i) Metaphase.
A tall man married á woman and all the off springs were tall. When the off
prings were selfed 75%of the outcome were tall.
Jsing suitable genetic symbols show;
1)The outcome of'erossing a tall man with a woman.
b) The Selfing of the off springs to have 75% of the outcome tall.
c) Explain why the offsprings from the marriage of the man and woman were all
tall.
END
HAPPY HOLIDAYS
Papont
Colapra
at conbly f
as He'teele oi do tys e
Speerh
arrow_forward
Q34
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1/outline
¥ Question Completion Status:
QUESTION 9
During metaphase II of meiosis,
O pairs of homologues chromosomes line up in the centre
O pair of sister chromatids line up at the centre
O Chromosomes are divided into two
O Chromosomes thicken
QUESTION 10
Which of the following is one of the body's first lines of defense against infection?
A) antibodies
B) obstacles such as skin and mucous membranes
C) increased production of certain hormones
D) decreased heart rate
O antibodies
obstacles such as skin and mucous membranes
O increased production of certain hormones
O decreased heart rate
Save
Shmtto sae and submit: Click Save All Answers to save all answers.
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A. Directions: Complete the following table by providing five applications of mitosis and
meiosis.
Mitosis
Meiosis
1.
1.
2.
2.
3.
3.
4.
4.
5.
5.
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QUESTIONS:
©A cell containing 20 chromosomes (diploid)
at the beginning of meiosis would, at its
completion, produce cells containing how
many chromosomes?
©A cell containing 40 chromatids at the
beginning of meiosis would, at its
completion, producể cells containing how
many chromosomes?
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11:51 B
+$.
← MitosisAndMeiosis.docx
AD
B
C
D
E
F
2. In what stage were most of the onion root tip cells? Does this make sense?
4. What would happen if mitosis were uncontrolled?
3. As a cell grows, what happens to its surface area-to-volume ratio (hint: think of a balloon being
blown up)? How does this ratio change with respect to cell division?
5. How accurate were your time predictions for each stage of the cell cycle?
&
ON 5G A
A C
6. Discuss one observation you found interesting while looking at the onion root tip cells.
Cell Cycle Division: Mitosis Beads Dia
ר
Dronhaco
EXPERIMENT 2: FOLLOWING CHROMOSOMAL DNA MOVEMENT
THROUGH MITOSIS
PAGE 5/...
OeScience Labs, 2016
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can you help please?
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Biology Unit Test Q3
Home
Insert
Draw
Layout
Review
View
Picture
alibri Regular (B
12
B
I
А.
23. What is the main reason why gamete cells undergo MEIOSIS instead of Mitosis? Explain
why.
a.
24. What is the main reason why somatic cells undergo MITSOSIS instead of Meiosis?
Explain and give an example.
a. .
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a. Explain how mitosis and meiosis are similar in their processes or mechanism using
two examples.
b. Explain how mitosis and meiosis are different in their processes or mechanism
using two examples.
**Please stay away from superficial statements such as both are a type of cell
division. We are asking for a more detailed example based on how the cells proceed
through the processes which has been highlighted in discussions, videos, and
readings.
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10:35 bb.
←
Which of the following statements
describes a process related to
reproduction?
During asexual reproduction, the
offspring are identical copies of the single
parent.
Nondisjunction is the process of joining of
two haploid gametes into a diploid cell.
During sexual reproduction, the offspring
are identical copies of the two parents.
Fertilization is the process of producing
diploid gametes from haploid cells
through cell division.
◄ Previous
Next ▸
Noll 58%
Not sa
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Instructions: Draw the images asked for. Given a hypothetical cell with
ploidy 2n = 6, draw the chromosomes as they move through the
different stages of mitosis and meiosis. The colors of the
chromosome represent a pair of homologous chromosomes. Please
include the colors of the chromosomes in you drawings. Solutions
to questions in this portion of the module are found in the university
learning management system.
In
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@1
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• Illustrate the chromosome changes in interphase and mitosis using a diploid cell that is 2n=4 (two
large and two small chromosomes).
• Label the circles as G1 and G2 stages of interphase and each phase of mitosis (in order).
Draw the chromosome in each circle using the following guidelines.
1) For unreplicated chromosome draw a line (/) and for replicated chromosome draw an X.
2) For simplicity, do not indicate that the chromosomes are decondensed chromatin during
interphase.
●
3) Skip drawing the nuclear membrane or spindle fibers, since we are focusing on the
chromosomes.
• Label genes in the resulting daughter cells as A/a and B/b. (These letters represent genes which are
at icular places along the DNA molecule. Each chromosomes can have a thousand genes but we
are focusing on these two.
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week 2 ( 4 - 6 )
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Procedure:
1. Draw a Punnett square which shows the inheritance of the sex
chromosomes. Represent the female sex chromosomes with XX and the
male sex chromosomes with XY.
Guide Questions:
a. What will be the sex of a child produced when an egg is fertilized
by a sperm that has a Y chromosome?.
b. What type of sperm must fertilize an egg to result in a female
child?
c. Based on this Punnett Square, what percent of children would you
expect to be male?
d. What sex chromosome is present in both male and female?
e. Infer which sex chromosomes determines a person's sex.
f. What are the other factors that may influence the expression of
human sexuality? .
arrow_forward
Instructions: Draw the images asked for. Given a hypothetical cell with
ploidy 2n = 6, draw the chromosomes as they move through the
different stages of mitosis and meiosis. The colors of the
chromosome represent a pair of homologous chromosomes. Please
include the colors of the chromosomes in you drawings. Solutions
to questions in this portion of the module are found in the university
learning management system.
Interphase
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12
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Please answer as comprehensive as possible.
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Fill in the blanks
*Items (1) to (23)
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- 11:51 B +$. ← MitosisAndMeiosis.docx AD B C D E F 2. In what stage were most of the onion root tip cells? Does this make sense? 4. What would happen if mitosis were uncontrolled? 3. As a cell grows, what happens to its surface area-to-volume ratio (hint: think of a balloon being blown up)? How does this ratio change with respect to cell division? 5. How accurate were your time predictions for each stage of the cell cycle? & ON 5G A A C 6. Discuss one observation you found interesting while looking at the onion root tip cells. Cell Cycle Division: Mitosis Beads Dia ר Dronhaco EXPERIMENT 2: FOLLOWING CHROMOSOMAL DNA MOVEMENT THROUGH MITOSIS PAGE 5/... OeScience Labs, 2016arrow_forwardPlease assist ASAP,a like is guaranteed!arrow_forwardcan you help please?arrow_forward
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