BIO101_Lab 9 Worksheets_Mitosis and Meiosis_Spring 2022

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BIO 101 Lab 09: Mitosis and Meiosis v.2021 63 BIO 101 Lab 09: Mitosis and Meiosis Objectives: Name the phases of mitosis and meiosis and describe their characteristics Identify the phases of mitosis as viewed through the compound microscope Explain the relationship between mitosis and the process of regeneration Compare meiosis I with meiosis II in terms of the position of the chromosomes in each stage, changes in chromosome number, and number of daughter cells produced Explain the process and importance of crossing-over between homologous chromosomes Compare the processes and end products of mitotic and meiotic cell division Background: Mitosis Many of us know that the outer layer of skin is subject to constant wear. To keep this protective layer intact, skin cells must be replaced throughout our lives. Cells of the epidermis, for example, are replaced every 25-45 days. Growth is another activity for which additional body cells are required. A zygote (fertilized egg) begins life as a single cell that multiplies into many cells as the embryo develops. During childhood, cell multiplication provides the many cells needed for growing into an adult. The type of cell division that makes growth and repair possible is called mitosis . All new cells are formed by the division of pre-existing cells. A key part of cell division is the replication of DNA in the parent cell and the distribution of DNA to the daughter cells . A cell is genetically “programmed” to carry out its function in the body. These instructions are found on the chromosomes . The normal number of chromosomes in a cell is referred to as the diploid number (abbreviated 2n ). Each type of organism has a characteristic number of chromosomes. The diploid number of chromosomes in human body cells, for example, is 46. The diploid number in cabbage is 18, in crayfish it’s 200, and in dogs it’s 78. After successful completion of cell division, each daughter cell must also be 2n as the parent cell was. To achieve this, the dividing cell passes through several recognizable stages during its life span. These stages constitute the cell cycle. During these stages, the dividing cells must solve several problems: Each new cell needs a full set of chromosomes. This is accomplished during Interphase of the cell cycle when chromosomes are duplicated during the S phase . During DNA replication, DNA is in a long thin uncoiled state bound to proteins. In this uncoiled state, DNA is referred to as chromatin . After DNA has been duplicated, the long threadlike chromatin coil and fold into compact structures. These condensed chromosomes are visible with a compound microscope. These duplicated chromosomes are referred to as sister chromatids . A cell preparing to divide will contain two complete sets of sister chromatids (one set for delivery into each of the two new daughter cells).
BIO 101 Lab 09: Mitosis and Meiosis v.2021 64 Sister chromatids must be attached together. A structure called a centromere fastens the duplicates together. This attachment makes it easier to keep track of the sister chromatids when they are sorted into two sets for delivery. The pairs of sister chromatids must be pulled apart and delivered to each new cell. A structure called the mitotic spindle hooks on to each pair of sister chromatids at the centromere. Spindle fibers pull the chromatids apart and deliver one chromatid from each pair to each new cell. The two cells must be separated into two daughter cells, each with its own set of chromosomes once the sorting and delivery process is complete. The process known as cytokinesis separates the cytoplasm into two halves. During cytokinesis in animal cells, the cell membrane pinches into a groove called the cleavage furrow . In plant cells, the presence of the cell wall causes the formation of a cell plate instead of a cleavage furrow. During this mitosis exercise, you will identify the various stages (Interphase, Prophase, Metaphase, Anaphase, and Telophase) of cell division. The following figure can be used as a guideline. Meiosis In contrast to mitosis, meiotic cell division consists of two nuclear divisions. This special type of cell division happens in the testes and ovaries of animals to allow the organisms to reproduce sexually. As you probably know, half of your genetic information (23 chromosomes) is inherited from your mother and the other half (23 chromosomes) from your father. To generate your diploid
BIO 101 Lab 09: Mitosis and Meiosis v.2021 65 number ( 2n ) of 46 chromosomes, the sperm and egg ( gametes ) must contain half the number of chromosomes, and therefore are considered haploid ( n ). When the diploid zygote is formed, the genetic information will determine all of the physical and physiological traits of the offspring. Meiosis is the process that generates a haploid gamete from a diploid germ cell . The stages of meiosis are similar to those of mitosis. The important differences include the following: The chromosome number is reduced from diploid to haploid to form gametes. An exchange of genetic material takes place during the process called crossing over . This results in genetically different gametes. Meiosis involves two cell divisions ( meiosis I and meiosis II ). Genes ( traits ) on a chromosome are often expressed in different forms called alleles . Individuals inherit two alleles for each trait, one from the mother’s egg and the other from the father’s sperm. These alleles from mom and dad are found on homologous chromosomes . Homologous chromosomes carry alleles for the same traits (face shape, eye size, etc.), although the genetic information is not identical. The combination of mom and dad’s alleles determines an individual’s genotype . If the two inherited alleles are different (Bb), an individual is considered heterozygous for that trait. The allele that is expressed in heterozygous individuals is referred to as dominant and is represented by a capital letter (B). The allele not expressed in heterozygous individuals is considered recessive and is represented by a lowercase letter (b). An individual with two identical alleles (BB or bb) is said to be homozygous for that trait. The physical manifestation of the combination of alleles is called the phenotype and is what we sometimes outwardly see. Sometimes traits have two possible phenotypes and other times there are three possible phenotypes. The traits with three possible phenotypes result from a different mode of inheritance called incomplete dominance . This type of inheritance occurs when an allele exerts only partial dominance over the other allele. This results in a blended intermediate phenotype in heterozygous individuals. In this exercise, you will simulate the sexual reproduction of jack jumper ants . To do this, you will work through the process of meiosis to form female gametes, decode the genetic information carried by these gametes, simulate fertilization, and use the genotype of a fertilized egg to predict the phenotype of the ant offspring. Jack jumper ants ( Myrmecia croslandi ) are native to Eastern Australia and are notable because they have the fewest possible chromosomes for a diploid organism, with their diploid number being 2 and their haploid number being 1. Another interesting feature is that only female ants perform meiosis to produce gametes. Male ants instead produce gametes using mitosis. This means that the processes of homologous chromosome pairing and crossing over will only take place in female ants. Additional information about jack jumper ants can be found at: https://www.antwiki.org/wiki/Myrmecia_croslandi Jack jumper ants have a number of traits that vary in their population. While some of this variation has been observed and studied, most of the genotypes and phenotypes we will “observe” in this laboratory activity have been invented for the purposes of this activity and should not be taken as actual facts.
BIO 101 Lab 09: Mitosis and Meiosis v.2021 66 The figure below shows a jack jumper ant with several of the invented traits indicated. For the purposes of this lab activity: The head size is either Large , Medium , or Small The body is either Hairy or Not Hairy The eye color is either Black or White The eye shape is either Oval or Round The Antenna length is either Long , Medium , or Short The body color is either Black , Brown , or Red The head color is either Gold or Black The leg color is either Orange or Black The ant either Does produce venom or Does Not produce venom Materials: Compound microscope Prepared slides of cells in various stages of mitosis Planaria Ice Petri dish with cover Razor blade Colored beads Plastic bag Bead connectors Dice Dissecting microscope
BIO 101 Lab 09: Mitosis and Meiosis v.2021 67 Safety: Follow all standard laboratory safety procedures. Handle the razor blade with care. Procedure: Mitosis Part 1 : 1. Use the mitosis figure in the Background section of this lab to answer the associated questions on the answer sheet. Part 2 : 2. Retrieve a compound microscope from the storage cabinet. 3. Obtain a prepared slide of mitotic cells and carefully secure it on the stage of the microscope using the metal stage clip. 4. Using the 4X scanning lens, find a cell in one phase of mitosis. 5. Increase the power to 40X and draw what you see as it appears under the microscope on your answer sheet. 6. Repeat steps 4 and 5 until all mitotic phases have been identified. Meiosis 1. Obtain a plastic bag from the supply bench. 2. To simulate the process of meiosis in jack jumper ants, you would perform the following steps: a. Obtain a plastic bag from the supply bench. b. Fill your bag with four beads from each jar. Note that there are two jars of beads of each color---striped and solid. The solid-colored beads represent dominant alleles and the striped beads represent recessive alleles for the same trait. c. Get two red centromeres and two yellow centromeres (bead connectors). d. Return to your lab bench and begin your experiment. 3. Determine if your imaginary organism will be male or female by randomly selecting a bead from your bag. Without looking in the bag, remove one bead from the bag. If the bead is solid-color, your imaginary organism is female, if the bead is striped, your imaginary organism is male. Record the sex of your imaginary organism and then place the bead you selected back into the bag.
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