Practice Exam 1

Select all CORRECT answer choices for the following statement: "Bacterial populations..." reproduce using spores similar to fungi engage in asexual reproduction using binary fission creating haploid bacteria reproduce by germination grow exponentially under optimal conditions grow more slowly if under nutrient depletion and other environmental stresses engage in sexual reproduction creating diploid bacteria

Please answer the fill-in-the-blank question using the answer key option. I have attached the image of both.

Can the Eukaryotic Cell (Animal) shape enclosed in the circle with all the shape's markings be drawn by hand? Note: The drawing should look like a student drawing and not an expert drawing, please make the marking on the drawing complete and in a clear line away from mixing words

Multiple antibiotic resistance genes are contained on a bacterial plasmid in a __________.     circular region of RNA     a series of interlocking proteins     the region that also contains genes for making the pilius needed to transfer the plasmid (Incorrect, already attempted)     the resistance cassette Thank you!

Row C D. B B. one: A C O phage with radiolabelled protein coat phage with radiolabelled DNA 100 10. phage infects The experiment shown above was designed by bacterium phage infects bacterium EXPERIMENT 1 phage shell is removed EXPERIMENT 2 요 Hershey & Hershey & Chase Meselson & Stahl Meselson & Stahl 8 phage shell is removed 28 no radioactivity in cells ii 48 radioactivity in cells LL and proved that ii DNA replication is semiconservative DNA is the hereditary material DNA replication is semiconservative DNA is the hereditary material (select the row that correctly completes the statement)

Figure shows a technique known as _______ ______.

Please can you draw the circled shape with all the shape's visas by hand Note: The drawing should look like a student drawing and not an expert drawing, please make the marking on the drawing complete and in a clear line away from mixing words Note: Please, please, draw by hand only. I do not want any drawing or electronic image of the figure

Based on the image below, answer the following questions:   Restriction enzymes PstI and ScaI were employed and the gel image after restriction digest is shown below.   The name of the plasmid is Blank 1. The gel lane on the Blank 2 was digested using PstI enzyme. (left or right) The gel lane on the Blank 3 was digested using PstI and ScaI enzymes. (left or right)

On Figure 1, what is occurring at the positions C indicated by black arrows?

Question is attached

Bacteriophage A average = 136 plaques Bacteriophage B average = 38 plaques Bacteriophage C average = 27 plaques Please help find the PFU/ml and answer the question on the best bacteriophage. Thanks

Can the Eukaryotic Cell (Animal) shape enclosed in the circle with all the shape's markings be drawn by hand? Note: The drawing should look like a student drawing, not an expert drawing

Define/Compare/Contrast/Distinguish these three terms: F plasmid F’ plasmid (and how is it formed) Hfr cell (and how is it formed)

Watch this video: https://youtu.be/a5jmdh9AnS41. How is the process of Polymerase Chain Reaction (PCR) like a copy machine for DNA?2. Describe how PCR can be useful when determining if a viral infection is present? I need help answering these two questions.

Can the Eukaryotic Cell (Plant) figure in the circle with all the figure's markings be drawn by hand? Note: The drawing should look like a student drawing and not an expert drawing, please make the marking on the drawing complete and in a clear line away from mixing words

Please help me with this question immediately within an hour or so

Telomerase contains both _______________ and _________________.   Question 14 options:   RNA and protein   DNA and protein   All of the above   RNA and DNA

I got these wrong can you please help? don’t give long explanations just help me finding right answers

QUESTION 13 A 2000 nt plasmid with 10 negative supercoils has L= 210 200 190 400

Please answer fast Which of these statements is false?   (Choose all that apply)   Group of answer choices   inclusions are membrane-bound organelles   Prokaryotes are haploid.   all cells have ribosomes   Cells that have plasmids often have hundreds of them within a single cell.   Plasmids are part of the chromosome.   Which of these statements are correct? (Choose all that apply)   Group of answer choices   The plasma membrane structure of most bacterial and eukaryotic cell types is a phospholipid bilayer   Only prokaryotic cells have a plasma membrane   some archaeal plasma membranes are lipid monolayers   The plasma membrane includes a diverse array of lipid and protein components

A piece of DNA ejected by a bacterial cell through a tube-like passage through the cell wall is called a ___.     chromosome     capsid     plasmid     chromatid

Plasmids are __________ DNA molecules that replicateautonomously and can be transferred between cells; theyoften contain genes for drug resistance or pathogenicity,or for specialized metabolic functions

1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?  2) Calculating Transformation Efficiency For the +DNA/+Amp/+IPTG plate, record the following:  Number of transformants (colonies): _________________ Nanograms of plasmid DNA added: 50 ng  Final recovery volume: 0.50 mL  Volume plated: 0.25 mL  Transformation efficiency equation: Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL) 3) Using the equation above, calculate the transformation efficiency. 4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?

Give only typing answer with explanation and conclusion Information: 1_Green Fluorescent Protein 2_nucleotide sequence, Amino acid sequence, and primers are obtained. 3_PCR protocol already described 4_bp has been calculations and estimated agarose gel image already designed. Questions: How do you analyze whether your target protein is expressed by E. coli cells. Explain your analysis method in detail and give information about the results you expect (in detail please)

________________ "cut" DNA at specific sequences and the resulting segment with sticky ends is "pasted" into a plasmid by ___________ to then be replicated in the host. Group of answer choices 1. Restriction enzymes; ethidium bromide 2 DNA polymerases; ligase 3 Restriction enzymes; ligase 4 Ligases; restriction enzymes

9. Please help answer this question and please show all work on how you got the answers. Thank you so much!! :)

Hello which one can be the right answer?

profile-image If you take a DNA photolyase- strain of E. coli, and subject it to the treatment below step 1, spread evenly on LB plate step 2, subject to UV light step 3, cover half of plate and grow in light What will be the most likely outcome if the level of UV is nearly lethal to cells on the plate? a. Only colonies on uncovered side of plate b. Only colonies on covered side of plate c. very few colonies in roughly equal numbers on both sides of plate

Question is attached,,,

Match the item to its best description or use. Agarose 1. produces DNA fragments RFLP 2. important tools; used to load samples into electrophoresis gels 3. small circular chromosomes in Comb bacteria 4. region of DNA that produces Micropipette different band sizes when cut with restriction enzymes Restriction Enzyme 5. produces wells in the gel where samples are added gel chamber 6. container where gel is placed to Stain or dye 8. Plasmid DNA introduce the electrical current 7. used to produce the actual gel used to visualize the otherwise colorless DNA bands

pXO1 and pXO2 are large plasmids – large pieces of DNA that are not the chromosome that can replicate independently of the chromosome. What can a Bacillus anthracis cell with a pXO2 plasmid do that a cell without pXO2 can’t? I need help answering this quwhstion as short as possible based of what is said in the paper

Drag and drop the appropriate text in the gaps below. Firstly, the plasmid DNA in the E. coli is propagated through Then the bacterial cells are pelleted by centrifugation at 10,000 RPM. The media supernatant is removed and the bacterial cells are The bacterial cells are then lysed via |. Contaminating macromolecules such as protein and chromosomal DNA is removed by Overnight incubation of the bacterial culture washed in cell resuspension buffer addition of lysis buffer addition of neutralisation buffer washing of the spin column separation via agarose gel electrophoresis