MICRO Aseptic Technique answer sheet (1)

plz answer all the questions plz and also state the aim for this lab .. thank u

LABORATORY REPORT: Aseptic Technique & Inoculation of Bacteria Guide Questions. 1. Why is direct flaming preferred when disinfecting loops and needles? 2. Why is it important to flame the entirety of the loop and not just the tip? What consequences can be seen when this process is not done correctly? 3. What is the difference between quadrant streak method A from method B? 4. Why do we use a pencil instead of a pen when labelling culture tubes or plates? Are there other alternatives in labelling? 5. Why is an inoculating needle used in Subculturing Techniques? Can a loop be used instead? NOTE: Please try to answer all of the question asked, i promised to give you a good ratings

LAB QUESTIONS – ASSESSMENT AND CRITICAL THINKING 1. When is a simple negative stain used? 2. Why do microorganisms remain unstained in the simple negative staining procedure? 3. What information can be obtained from a simple positive staining procedure? 4. What is the difference between a simple and differential stain? 5. What is the reagent and purpose for each of the following gram stain steps? a. Primary stain b. Mordant c. Decolorizer d. Counterstain 6. Which step is the most crucial or most likely to cause poor results in the Gram stain? Why? 7. What part of the bacterial cell is most involved with Gram staining, and why? 8. What cell wall component is responsible for the acid-fast property of mycobacteria? 9. Is a gram stain an adequate substitute for an acid-fast stain? Why or why not? 10. Which area on a streak plate will contain the greatest amount of growth? The least amount of growth? Explain your answers. 11. What is a colony (as viewed on an agar plate)? How can a pure…

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Give the uses/functions and images of each apparatuses. Basic Laboratory Equipment Uses/Functions Picture   1. Microscope     2. Colony Counter     3. Autoclave     4. Microbiology incubator     5. Drying oven     6. Refrigerator (microbiology)     7. Bunsen burner/alcohol lamp     8. Candle jar     9. Anaerobic jar     10. Microhood or Bacteriologic hood/Safety hood/Safety cabinet

Please answer the questions in the grey colored box provided in the image.

Separation of Amino Acids by Thin Layer Chromatography Lab Questions 4. Why is it necessary to run TLC in a closed container and have the chamber saturated with solventvapor? 5. Why must the spot be applied to the TLC plate above the level of development solvent? 6. What will be the result of adding too much sample to the TLC plate?

ANSWERS TO QUESTIONS ON LABORATORY ASSAY NO.2 1. Why is it necessary to calibrate a Pasteur pipette? 2. Cite some possible sources of errors in the calibration of Pasteur Pipette. 3. Cite other procedural techniques that can be used for the calibration of Pasteur Pipette.

Question 53 Which of the following risk factors would prompt a nurse to advocate for a spirometry test for a COPD diagnosis? Question 53 options: Frequent respiratory tract infections History of smoking and over the age of 40 Daily puffer use Persistent cough for the past 2 weeks

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I need help with microbiology/2010 1. Describe where the following items should be discarded: a) gloves b) petri dishes   c) test tubes   d) microscope slides   2. Describe the safety procedures for the following hypothetical situations: a. You spilled a full test tube of bacterial culture on the bench top.   b. You notice flames coming from the tubing of your Bunsen burner

Question:- Describe control strains used in the clinical microbiology laboratory and explain their maintenance in the laboratory. ( write BY WORD and all steps I need). Introduction Discussion References

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QUESTION: Microbes from a blood culture inoculated with blood drawn from a catheter were determined by the appearance of growth on Mannitol Salts Agar and through other tests to be Staphylococcus epidermidis. It can be concluded that: a.  a bloodstream infection caused by S. epidermidis is occurring in the patient from whom the blood was drawn b.  The bacteria fermented mannitol, changing the color of the growth medium from pink to yellow. c  S. epidermidis is present in the sample as a normal flora contaminant d.  That in order to determine if a bloodstream infection is present, these results must be compared to a blood culture of a sample drawn from a peripheral vein

this lab was carried out the results  are in the picture below plz provide a aim for this lab abd answer thw questions below  the method used is also stated  Title: Bacterial Inhibition Aim:    Materials: Streak plate from LAB # 3 Disks Ethanol (dilutions of 20%, 50%, 70%, 95%) Burner Flame Matches Tape Method: Ethanol was used to clean the work station A maker was used to dived each equal sections and ruler and was label with appropriate concentrations. The plate carefully open The disc was soak in the different concentrations of ethanol Placed one disc per concentration for each labelled section The plate was tape shut The plate was returned to the incubator The zone of inhibition was recorded after 3 days. question 1 state a aim  question 2 in a table format plz explain the bacteria growth of e.coil and staph and state any two limation of this lab

Give the uses/functions and images of each apparatuses. Basic Laboratory Equipment Uses/Functions Picture 6. Refrigerator (microbiology)     7. Bunsen burner/alcohol lamp     8. Candle jar     9. Anaerobic jar     10. Microhood or Bacteriologic hood/Safety hood/Safety cabinet     11. Bacteriologic filters (Seitz, Chamberlain, Berkfield)     12. Petri dish     13. Culture tubes     14. Hanging drop slide     15. Durham’s tube

Question:- Your medical care team determines that a third patient's severe illness is caused bya helminth. Which technique or techniques described above would you use to identifythe helminth? Justify your answer.

Practice Question Chapter 3 Another source of contamination cheesemakers really worry about are bacteriophages. Entire batches of cheese can be ruined in a matter of days by bacteriophages infecting lactic acid bacteria. 4. Should cheesemakers be worried about endospore growth of Gram-positive cells? Why or why not?

Differential staining procedures gray stain and acid-fast stain lab report.

Give the uses/functions and images of each apparatuses Basic Laboratory Equipment Uses/Functions Picture 21. Antibiotic disk     22. Antibiotic dispenser     23. Weighing balance     24. Forceps     25. Stains/Staining reagents     26. Culture media/Culture plates     27. Culture swabs     28. Gloves     29. Mask     30. Caliper ruler

INSTRUCTIONS: Please do not copy here in Bartleby or in Google. QUESTIONS : 1. Aside from using the autoclave, what are other ways to kill microorganisms from glassware that can be use in the laboratory?