W1 Lab

.docx

School

Chamberlain College of Nursing *

*We aren’t endorsed by this school

Course

252

Subject

Biology

Date

Jan 9, 2024

Type

docx

Pages

Uploaded by AmbassadorArt12622

Week 1 Lab Instructions Lab Safety / Muscle Structure Activity Deliverable Points Part A Basic Laboratory Information and PPE Part B Lab Safety Training and Certification: Digitally Submitted Not Graded but Required Part C Introduction to the Histology of Muscles 10 Part D Muscle Activation Lab 20 All Lab Deliverables Complete Parts A, B, and C 30 References: 1. Saladin Anatomy & Physiology: The Unity of Form and Function Part A: Receive Basic Lab Information and PPE Obtain overview of physical laboratory, facility and receive PPE – disposable laboratory jacket. 1.0 – Attend first physical laboratory during Week 1 1.1 – Pay attention to the instructor 1.2 – Take notes concerning fire exits, fire extinguisher, eye bath, emergency shower, chemical spill kit, red bag waste, broken glass waste, normal waste and MSDS handling documents 1.3 – Receive a disposable laboratory jacket and storage bag to reuse the jacket until it becomes visibly soiled. Part B: Complete Lab Safety Training and Certification Laboratory Safety Training Procedures, Lab Safety Critical Thinking Questions, and Certification Statement Review the Laboratory Safety Training Slides located in the Files 2.0– Review the slides 2.2 – Complete the attached Laboratory Safety Critical Thinking questions 2.3 – Double check your answers to the safety critical thinking questions and submit completed worksheet to your professor or upload into Canvas 2.4– Complete and sign the Lab Safety Training Questions and Sign Digitally before coming to your scheduled lab time in week 1. BIOS252 Week 1 Lab Instructions (B) Page 1 of 10

Part C: Introduction to the Histology of Muscles BACKGROUND – HISTOLOGY OF MUSCLES In the human body, muscles provide the ability of the body to move. Muscles are cells that alternate between contraction and relaxation. This alternation allows the muscles to produce work including but not limited to:  Producing body movements  Stabilizing body positions  Storing and moving substances within the body  Generating heat Muscles have four unique properties to help them contribute to homeostasis in your body:  Electrical excitability  Elasticity  Contractility  Extensibility Muscles or myocytes are divided into three categories based on both their structure and their function: 1. Skeletal muscle 2. Cardiac muscle 3. Smooth muscle Skeletal muscle – is commonly found attached to bone or skin. This type of muscle is responsible for voluntary movement and posture. Skeletal muscle is commonly described as being: long, thin, cylindrical, multinucleated, and striated (stripped in appearance). Skeletal muscle is arranged into contractile units known as sarcomere s. This arrangement allows for the contractile proteins actin and myosin to slide (move) on top of one another causing the muscle to shorten. This is known as the Sliding Filament Theory . BIOS252 Week 1 Lab Instructions (B) Page 2 of 10 a. Skeletal Muscle Striations Multinucleated

Cardiac muscle – is found in the heart. This muscle is in control of the involuntary contraction and control of the heartbeat. Cardiac muscle is commonly described as being: short, fat, branched, uni-nucleated, and striated. Cardiac muscle is found attached to intercalated discs which are part of the hearts exoskeleton. Since cardiac muscle is also striated like skeletal muscle, the intercalated discs are the tissue that the cardiac cell and pull against allowing the cell to shorten when contracted. Within the heart, dispersed between cardiac muscle fibers, you will find the electrical conduction system of the heart. This electrical conduction system allows for the heart to maintain an auto-rhythmicity. Smooth muscle – is commonly found in hollow organs, the skin, and blood vessels. This muscle is in control of the involuntary movement of substances within those organs. Smooth muscle is generally individual cells that connect together through gap junctions creating an intricate network of muscle cells that can pass action potentials from cell to cell. Smooth muscle contains the contractile proteins of actin and myosin but they are not arranged into sarcomeres so smooth muscle DOES NOT contain striations. BIOS252 Week 1 Lab Instructions (B) Page 3 of 10 Nucleus Intercalated Discs Striations Relaxed Contracted

PURPOSE: In this lab, you will be asked to look at and describe the three different types of muscle: skeletal, cardiac and smooth, on a histologic level. MATERIALS:  Microscope  Slides of each muscle type: skeletal; cardiac; smooth  A writing utensil of some type (pen, pencil, or colored pencils)  Digital camera or mobile device capable of taking photos  Printed copy of the lab PREPARATION: 1. Read your lab in its entirety before coming to class. 2. Clear your workstation of all unnecessary materials. Book bags and or purses should be hung on hooks or places at the front of class. Make sure all other unnecessary materials (coats, drink containers, unused textbooks, etc.) are all stored and placed in a safe area out of the way. 3. Obtain all materials listed above. 4. Familiarize yourself with your microscope. Make sure it is plugged in and the microscope is in working order. Make sure the light is set to your preferred brightness. Make sure you are aware of where all the adjustment knobs are located and what they do. 5. Be aware of the instructions for documenting your lab work. You will be drawing what you see in the microscope so make sure you have a printed copy of your lab with you as well as your writing utensil(s). ACTIVITY: 1. Observe each of the three types of muscles under the microscope. 2. Make sure to center the muscle each time you are observing them. 3. Use different magnifications to view each muscle type and make sure to note which power gives you’re the best focus. This magnification will be indicated in the Observation Report section of this lab. 4. Make note of what makes each muscle type stand out and record those finding in your Observation Report. What kind of details can you observe? 5. Draw and take a picture of what you can observe. The drawing will be done in the appropriate box in the Observation Report. All photos need to be attached to the submission when turning the assignment in or can be inserted directly into the Observation Report. 6. When finished, make sure to return all items to their appropriate storage place and clean your station with bleach water. Dispose and materials that need to be disposed in accordance with the MSD sheets found at the front of the classroom. 7. Submit your completed lab with pictures to the Week 1 Lab hyperlink in the Unit 1 module. OBSERVATION REPORT: Take a picture or draw a sketch in the appropriate box below. Make sure to indicate the total magnification you used for your observation: total magnification = ocular lens power x objective lens power. BIOS252 Week 1 Lab Instructions (B) Page 4 of 10

Your preview ends here

Eager to read complete document? Join bartleby learn and gain access to the full version