Gel

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Liberty University *

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MISC

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Biology

Date

Jan 9, 2024

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pdf

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2

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The Electrophoresis Lab Worksheet Student Name: _____________________________________ Date: __________ Directions: Use the information in the “Electrophoresis Lab” lesson to help you complete this handout. Once you have completed this handout, save it to your device, and upload it to the appropriate assignment. 1. How can you sort and measure the DNA strand in the tube? (5 pts) 2. What other molecules, other than DNA, is gel electrophoresis helpful for separating? (5 pts) 3. What is added to electrophoresis to make the DNA move? (5 pts) 4. Why do scientists stain the DNA when using gel electrophoresis? (5 pts) 5. List the 5 steps of this gel electrophoresis lab. (10 pts) 6. List the 6 tools you need to start the lab investigation. (10 pts) 7. Where do you add the DNA samples? (5 pts) 8. What piece of lab equipment/tool do you use to transfer the DNA samples into the wells? (5 pts)
9. When you prepare to run the gel, remember the black end will generate a ________________ charge, and the red end will generate a ______________________ charge. (8 pts) 10. Which cord must you put closest to the wells to move the DNA through the gel? (5 pts) 11. Describe what happens when the power is on, and the electrical current goes through the electrophoresis box. (9 pts) 12. Which DNA strands move through the gel quickly and how do you know? (8 pts) 13. What stain are we using to visualize the DNA? (5 pts) 14. Record your 3 DNA strand length estimates below: (5 pts) 15. Use the picture labeled “DNA Fingerprinting” from the lesson to help you identify which of the children (labeled C1, C2, C3, and C4) has a DNA fingerprint most closely resembling their father (F). (10 pts)
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