F23 Growth_ws (1)

Hello, please read the attached Microbiology question and answer the question and its two parts correctly. Please determine the dilution factor / final dilution based on the question. You do not have to give me a long explanation as to how you got the answers.  *If you correctly answer the question, I will provide a Thumbs Up to you. Thank you.

Hello, please read the attached Microbiology question and answer the question correctly.  *If you correctly answer the question, I will provide a Thumbs Up to you. Thank you.

answer the following questions with book reference. 1. What is meant by a bacterial “colony” or colony-forming unit? 2. Why are colonies that develop on a heavily seeded plate smaller than those that appear on a sparely seeded plate? 3. What are some of the uses and advantages of the ff.:a. Agar plateb. Agar slantc. Broth culture 4. What are the advantages and limitations of studying bacteria by means of the Culture method? 5. Why is it desirable that most cultures be inspected after 15 to 18 hours of incubation? 6. Why should culture media after inoculation be incubated at an optimal temperature immediately? 7. Describe other types of aerobic jars and anaerobic methods.

Topic: Preparation of Microbial Suspension A Pure colony is needed for inoculation in sterile water, a small portion of culture media was included. Effect:  Rationale:

A 1.5-year-old child developed vomiting, diarrhea, and fever. Stool sample were inoculated into the Endo media. After 18 hours on the surface of the medium grew medium-sized, round, slightly convex red colonies with a metallic luster. The doctor suspected Escherichia coli. 1. Name the composition of the Endo agar media. 2. Describe the properties of bacterial colonies on the Endo media.  3. What purpose differential diagnostic Endo media used for?

Activity 2. Media Used in Isolating Coliforms You are a group of microbiologists tasked to test the presence of coliforms in the newly built water station. You are asked to isolate E. coli, Salmonella, and Shigella. Enumerate the media used for each step of isolating these bacteria. Include pictures and references. Bacteria Enrichment (Broth/Agar) Presumptive test (Broth/Agar) Isolation Media (Broth/Agar) E. coli Salmonella Shigella 1. How can you confirm that E. coli, Salmonella, and Shigella are present in the newly built water station? Explain. 2. What is the difference between nutrient broth and nutrient agar? 3. What is the importance of these steps (Enrichment, Isolation, and presumptive test) in isolating bacteria? Conclusion about the process of isolating bacteria

can you please do #1

Questions 1. Explain why it was important to transfer the smallest colonies of yeast to the selective media plates.

Subculture and Colony Morphology Descriptions Following identification of the Gram-negative isolate and Gram-positive isolate, you next subculture each onto fresh nutrient agar plates.  Briefly describe the subculture process in three or so sentences and what this allowed you to achieve; follow this with colony morphology descriptions of each.    Description:           Isolate A – Describe:    Colony morphology:    Medium & incubation temperature: Isolate B – Describe:    Colony morphology:    Medium & incubation temperature:

Please explain

Answer the following questions:   1. Define a bacterial colony.   2. What is the difference between macroscopic and microscopic appearance of bacteria?   3. State the three standard terms used to describe single colonies on agar plates.   4. State and define the three types of growth that may be seen in a broth culture.   5. State three basic shapes of bacteria.   6. State and describe the different arrangements of cocci.   7. What is the difference between true motility and Brownian motion?

Using the attached image, explain the observed results on the NA and ECM plates. Be sure to address why each sample is either able or unable to grow on each plate and include the genotype(s) of the cells which are capable of growth.

Hello, please answer the following Microbiology question correctly and fully. Please explain your answer. Question: Why is it necessary to inoculate the KIA medium all the way down to the bottom of the tube during inoculation? (What's different about the top and the bottom of the tube?)  *If you correctly answer the question correctly, I will provide a Thumbs Up to you. Thank you.

Guide Questions: Differentiate the types of culture media based on its physical state and uses. What makes agar good support for microbial growth?

Greetings :) Based on the results of using Hanging-drop method in Figure 2, what microorganisms can you find? Kindly state or identify all microbes you can see, and briefly described each of them. Thank you so much!

I. Match the bacterium being described in column X with its best classification found in column Y. Choose the best answer.  Column X1. Staphylococcus aureus can be grown on mannitol salt agar that contains 7.5% NaCl. The bacterium is________. 2. Lactobacillus acidophilus grows best at pH 4.5. It is considered a(n) ________. Column Y A. acidophile B. alkalophile C. barophile 3. A bacterium that thrives in the Great Salt Lake but not in fresh water is probably a ________.4. Bacteria isolated from the bottom of the ocean need high atmospheric pressures to survive. They are________.5. A bacterium that thrives in a soda lake where the average pH is 10.5 can be classified as a(n) ________. D. halotolerant E. halophile

What Gram-negative bacterium might you come into contact with in a unsanitary hot tub?    asap please  detail explanation needed

I need help with question 1

Background: The following results were obtained from a broth dilution test for microbial susceptibility. What is the minimum bactericidal concentration of this antibiotic and explain what you based your answer on?

Title: Spoilage of coconut water Aim:  To assess the diversity of psychrotrophic bacteria causing spoilage in coconut water. How might incubation of plates at 37°C influence the results of the quantitative dilution and plating of the coconut water samples?

Please answer fast Dilution Problem. A culture of Staphylococcus is diluted as follows: (1) 20mL are added to 80mL of water. (2) 10uL from (1) are then added to 9.99mL of water. (3) A 10-2 dilution is made from tube # (2). (4) 100uL from (3) are plated for a pour plate and incubated.   Growth Problem.A culture with approximately 2x103 cells/mL were incubated. After 3 hours, the number of cells had increased to 3x105. a) How long was the generation time in minutes?b) How many generations have occurred

Hello, please read the attached Microbiology question and complete the entire chart given correctly. *If you correctly complete the entire chart with the correct answers, I will provide a Thumbs Up for you Thank you.

Kindly answer this question

Please use the first image as an example to fill in the gap for the 2nd image .please no much explanation.Thanks

Highlighted question

URGENT

"Microorganisms used a biological warfare." a. What is the scientific name of the microorganism/s and its general characteristics?b. Why those microorganisms are used in such process/product?c. How do/does the microorganism/s impact the lives of human?

a. What oxygen requirements do your think this bacterium will have (obligate aerobe, obligate anaerobe, facultative anaerobe, aerotolerant anaerobe, or microaerophile), and why? b. You run a thioglycollate test of your isolated bacterium. Based on your response to Part A, which thioglycollate tube best represents the result you would expect for your bacterium? Explain your response. If the result you would expect is not represented, explain what the tube should look like. c. What would you expect to be the result of a catalase test of your bacterium? d. What techniques or specialized equipment, if any, would you need to grow cultures of this bacterium?

Answer the following questions briefly and concisely 1.How do bacteria in a chemostat and those in a batch culture vary from one another? 2.  What happens in a chemostat if the dilution rate is higher than the organism's maximum specific growth rate? 3.Does a chemostat require the use of pure cultures? 4. Why would a complicated culture media for Leuconostoc mesenteroides be simpler to make than one with a fixed chemical composition?

Some Metabolic Activities of Bacteria A. Questions: 1. List 5 importance of using selected and differential culture media. 2. Mannitol Salt Agar is a differential or selective media? Explain why? 3. What enzyme and explain the principle on how Simmons citrate agar change in color for positive result? 4. What two groups of bacteria that can be differentiated with the catalase test?

PLEASE answer the 2 with no much explanation Culture media is sterilized after the inoculation of microbes to ensure optimal growth of target microorganisms True False     Microwave oven available in the laboratory can be used to heat culture media, as well as food for lunch. True False

A class of 15 students (8 males and 7 females) will need to culture an unknown bacteria for a specific activity where 5 nutrient agar pates per female student and 3 agar slants per males student are needed to prepare. agarplate: 25mL Agar slant: 10mL Broth tube: 8mL Nutrient Agar: Yeast extract: 2g/L peptone: 5g/L Sodium chloride: 5g/L Agar powder: 15g/L