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Clinical Laboratory Techniques 1- Lab
Urinalysis on an Abdominal Urine and Centrifuge
Destinee Mills
Introduction A centrifuge is used to separate materials that have different densities by applying force and rotating balanced units at various speeds. The force and speed required to separate materials depends on the size and viscosity of the specimens. A clinical centrifuge tends to operate at slower speeds and relative centrifuge force (RCF) and accepts a range of heads and cups to accommodate the various array of laboratory specimen’s containers such as serum tubes, bacteria bottles, blood bags, and urine cups/urinalysis tubes. Specifically, the separation of urine by centrifugation is performed so that the liquid within the urine can be separated to reveal any solid components that may be in the sample such as crystals, blood, or microorganisms. The purpose of this lab was to learn how to use a universal tabletop centrifuge and prepare two abnormal urine samples from that can be evaluated and compared to one another.
Materials Universal Centrifuge
Disposable gloves
Clinitest Dipstick
Refractometer
Acetone
Transfer pipettes
Water
Urinometer
Urine sample C
Urine sample B
Acetest Tablets
Clinitest Tablets
20% Sulfosalicylic Acid Test tubes
Microliter Pipette Absorbent Mats
Kim Wipes
Dry paper towels
Procedures 1.
Prepare a clinical centrifuge for the use, centrifuge the urine samples, centrifuge a sample, and separate the specimens. 2.
Perform a routine urinalysis (physical and chemical) test on abnormal urines C & B and record all
results. 3.
Perform Clinitest
4.
Perform Acetest
5.
Perform SSA test
6.
Perform Ictotest
7.
Properly use a automated instrument to analyze urine specimens (Clinitek)
8.
Properly use a refractometer to check the specific gravity of urine. 9.
Record all test results as they obtained from the performed tests.
Results Urine # 1- Sample C TEST
POS OR NEG OR UNITS
COLOR
Color
N/A
Straw
Transparency N/A
Clear
Specific Gravity (Refractometer)
N/A
1.010
pH
Units= 7
Light green
Protein
Negative
Yellow
Glucose
1000 md/dL
Dark green Ketone
Negative
Tan
Blood
Negative
Yellow
Bilirubin
Negative
Tan
Urobilinogen Normal Light Pink
Nitrite Negative
White
Leukocyte Esterase Negative
White
Specific Gravity (Strip)
Units= 1.010
Light green
Clinitest
2%
Dark orange
Sulfosalicylic Acid Test
Negative
No turbidity Acetest
Trace
Light purple speckles
Icotest
Negative
No color reaction Urine # 2- Sample B TEST
POS OR NEG OR UNITS
COLOR
Color
N/A
Red
Transparency N/A
Clear
pH
Units= 7
Light green Specific Gravity (Refractometer)
N/A
1.015
Protein
Negative
Yellow
Glucose
Normal
Yellow
Ketone
Trace
Pink
Blood
Negative
Yellow
Bilirubin
++
Med. Pink
Urobilinogen Normal
Light Pink
Nitrite +
Pink
Leukocyte Esterase +
Lavender
Specific Gravity (Strip)
Units= 1.015
Army green Clinitest
Negative
Dark blue
Sulfosalicylic Acid Test
Negative
No turbidity Acetest
Trace
Light purple speckles
Icotest
Negative
No color reaction
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