Module 5 Lab Report

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2045L

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Chemistry

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Feb 20, 2024

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Module 5: Food Dyes Analysis in Commercial Products CHM 2045L

Introduction: The objective of this experiment is to use the principles of spectrophotometry for chemical analysis and determine the concentration of food dye in different commercial products. The different concepts of this experiment include visible spectrum and colors. The visible spectrum is the segment of the electromagnetic spectrum that is visible to the human eye. What is seen in this segment are colors. Red, orange, yellow, green, blue, indigo, and violet are what can be seen by the human eye. Another concept is ultraviolet-visible (UV-Vis) spectroscopy. This is a technique where light is passed through a sample at a specific wavelength in the visible spectrum. This helps to determine the transmission and absorbance of a solution. In this experiment, we will create a calibration curve. A calibration curve is created by preparing a set of standard solutions with known concentrations of the analyte. Then the instrument response is measured on the y- axis as the concentration of each solution is measure on the x-axis. Beer’s Law is an equation (A= ε l c) that is made by combining the relationships of the cell path length and the analyte concentration of absorbance. Experimental Methods: 1. Start with a red 40 stock solution. 2. Measure 10 mL of this solution into a graduated cylinder. 3. Transfer that into a 25 mL volumetric flask 4. Rinse the graduated cylinder with distilled water to ensure that all the stock solution is transferred. 5. Fill up the rest of the volumetric flask to the line with distilled water. 6. Cap the flask and invert the solution a few times until the solution is homogeneous. 7. Transfer the solution into a 50 mL beaker 8. Rinse the volumetric flask with distilled water to avoid cross contamination. 9. Take 10 mL of the diluted solution and put it back into the flask 10. Repeat earlier steps to perform serial dilution. 11. When done with dilution, take a small amount of all new solutions and place them into small, clear plastic cuvettes. 12. Calibrate the spectrometer.

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