Lab Report 5_CH 237-016
docx
School
University of Alabama *
*We aren’t endorsed by this school
Course
237
Subject
Chemistry
Date
Dec 6, 2023
Type
docx
Pages
4
Uploaded by SargentProton21896
Patti Smith
CH 237-016
TA: Deblina Bhowmick
3/2/23
Experiment 5: Thin Layer Chromatography Analysis of Drug Compounds
I. Introduction
This lab addresses the composition of various unknown analgesic drugs. There are four
standard components to this type of mixture: aspirin, acetaminophen, ibuprofen, and caffeine.
Each of these compounds contain unique properties that can provide information about
unknown compounds when compared side by side. Through these comparisons, three unknown
drugs will be identified per the goal of this experiment. According to the literature, the
unknowns may be as follows: Advil (200mg Ibuprofen), Anacin (400mg Aspirin, 32mg Caffeine),
Aspirin (325mg Aspirin), Excedrin (250mg Aspirin, 250mg Acetaminophen, 65mg Caffeine), or
Tylenol (325mg Acetaminophen).
In order to compare the standards to the unknowns and determine which drugs are
being tested, a variety of techniques are used. A small spot of each solution (three unknowns
and the four standards) is added in its own section to the starting line of a silica gel thin layer
chromatography (TLC) plate with a fluorescent indicator. The TLC plate is developed in 200:1
ethylacetate/acetic acid and then allowed to dry. Once dry, short-wavelength (254nm) UV light
is used to identify the centers of intensity for each spot. These intensity centers are compared
so as to identify each component. Each standard shows a unique spot placement on and the
unknowns reflect this with their component(s).
II. Results
R
f
(aspirin): 2.4cm/2.9cm =
0.83
R
f
(acetaminophen): 1.7cm/2.9cm =
0.59
R
f
(ibuprofen): 2.6cm/2.9cm =
0.90
R
f
(caffeine): 0.6cm/2.9cm =
0.21
R
f
(unknown 1)
1
st
spot: 0.5cm/2.9cm =
0.17
2
nd
spot: 2.1cm/2.9cm =
0.72
R
f
(unknown 2)
1
st
spot: 0.6cm/2.9cm =
0.21
2
nd
spot: 1.7cm/2.9cm =
0.59
3
rd
spot: 2.1cm/2.9cm =
0.72
R
f
(unknown 3): 2.5cm/2.9cm =
0.86
III. Discussion
A TLC was run in order to identify the presence of various standard active ingredients in
three unknown samples. Comparisons are made between the unknowns and standards through
the calculated R
f
values.
The R
f
values and molecular structures communicate a great deal
about each standard. It can be assumed that of the four standards caffeine is by far the most
polar, followed by acetaminophen, aspirin, and ibuprofen. Compounds that are highly polar are
highly retained in the TLC which is shown by a low R
f
value, such as that of caffeine (0.21). The
molecular structure of caffeine contains a good amount of both nitrogen atoms and carboxylate
groups, which are more polar than carbon and pull electrons closer through lone pairs. Caffeine
is also bicyclic, which can increase polarity.
Acetaminophen and aspirin contain a similar number of polar atoms to each other, but
less than caffeine, which could explain some of the similarities and differences in polarity. The
differences mainly come in the presence of a benzene ring in acetaminophen and aspirin, while
there is not one present in caffeine. The lone pairs of nitrogen and electronegative hydroxyl and
carbonyl groups of acetaminophen make it more polar than the ester of aspirin. Ibuprofen is
much less polar than any of the other standards because it lacks multiple polar functional
groups.
The observed R
f
values for the unknown components are not exactly the same as the
standards, but they are comparable enough to infer. For example, the first spot of unknown 1
had an R
f
value of 0.17 which is slightly lower than the caffeine standard (0.21). However, these
two values are close enough to each other that it can be assumed that the unknown is caffeine.
The discrepancies between the two values are likely due to some spots overlapping during the
spotting process as some spots were larger than desired. Overlaps could cause the TLC to
appear as if other compounds were in a row that they were not or slightly affect the solutions
polarity. Both could result in the TLC being less accurate. Other than the possibility of initial
overlap, there were not many noticeable errors as the solvent did not reach the start line and
wash away any spots.
IV. Conclusion
The R
f
values for unknown 1 align with aspirin and caffeine at 0.72 and 0.17 as compared
to the standards of 0.83 and 0.21. Unknown 2 shows caffeine, acetaminophen, and aspirin at
0.21, 0.59, and 0.72. The standard values for these components are 0.21, 0.59, and 0.83,
respectively. Unknown 3 contains one component determined to be aspirin at 0.86 which is
slightly higher than the standard of 0.83. From this data it can be determined that unknown 1 is
most likely Anacin, unknown 2 is Excedrin, and unknown 3 is Aspirin. Despite not perfectly
aligning on the TLC, this interpretation should accurately reflect the identities of the unknowns.
V. Additional Questions
1) What would have happened to the Rf values of the 4 components if the TA had accidentally
made the solution using diethyl ether instead of ethyl acetate?
If the TA had prepared the eluent solution with diethyl ether instead of ethyl acetate, the
R
f
values would likely appear to be much lower than they should. Diethyl ether is less
polar than ethyl acetate because it is not an ester. This lower polarity would cause the
spots to move less than if they were in the presence of an ester. It is possible that the
spots may not move at all if diethyl ether is not polar enough. This could affect the
accuracy of the TLC interpretation.
2) Suppose that the generic acetaminophen that we used actually contained phenacetin (see
experiment 1 for the structure). Would you expect phenacetin to have a higher or lower Rf value
than acetaminophen. Briefly explain your answer.
Phenacetin would likely display a slightly higher R
f
value than acetaminophen if it was
set as the acetaminophen standard. This difference is due to small discrepancies in the
molecular structures of each compound. Acetaminophen has an alcohol group, but
phenacetin contains an ether with an alkyl group attached (this takes the place of the
original hydrogen). The alkyl group makes phenacetin slightly less polar than
acetaminophen which, in turn, causes it to travel slightly farther and show a higher R
f
value.
3) Another common pain reliever, Aleve, contains naproxen sodium as the active ingredient. Find
and draw the structure for naproxen sodium. The physiological effects of naproxen sodium are
very similar to one of the compounds analyzed in this experiment. Which one do you think it is,
and how are they structurally related?
The structure of naproxen sodium is seen below. It is most similar in physiological effects
to ibuprofen. They are somewhat similar in structure as both contain at least one
benzene ring. Naproxen contains two while ibuprofen only has one, but both have
functional groups in similar spots (across from each other on the ring). These groups are
not exactly the same, but they do share similar properties, such as a carboxyl group.
Your preview ends here
Eager to read complete document? Join bartleby learn and gain access to the full version
- Access to all documents
- Unlimited textbook solutions
- 24/7 expert homework help
4) List three common applications for thin layer chromatography.
TLC is a highly versatile separation method. It can be used to analyze virtually any
substance class, including pesticides, steroids, alkaloids, lipids, nucleotides, glycosides,
carbohydrates, and fatty acids. A common application for TLC is the analysis of drug
residues and antibiotics in food and environmental samples. It can also be used to
identify and quantify colors, ingredients, preservatives, and sweetening agents in food
and cosmetic products. Another common use of TLC is in the quality control and purity
testing of pharmaceutical formulations.
Related Documents
Related Questions
PART ONE: Preparation of FECNS* Solution
Prepare the two solutions in table 1 by accurately measuring the required volumes of distilled
water and CNS into two labeled test tubes. The absorbance of the solutions must be measured
soon after adding the Fe" solution from a buret.
TABLE 1
Fe
5 ml
5S mL
Solution
Distilled Water
CNS
2 mL
Total Volume
3 ml
2 ml
10 ml
3 mL
10 ml
In this part of the experiment you are to explore what the above quote means. As a
PRELIMINARY Exercise, formulate a hypothesis as to what would happen under each of the
following circumstances:
1. 1ml of 0.0020 M CNS' solution is added to the unused portion of
solution 1 in the test tube
2. One drop of 0.20 M CNS solution is added to the unused portion of
solution 2 in the test tube.
arrow_forward
How does ammonia, dimethylglyoxime (DMG), and 8-hydroxyquinoline (8HQ) interact with your cations, leading to the evolution of colored species?
Context: Paper chromatography experiment (separation of inorganic cations) with a 9:1 acetone/ HCl solvent
arrow_forward
Identify the detectors in gas chromatography that can be used for the detection of each type of analyte. More than one
detector may be appropriate for a given analyte, and a given detector may be appropriate for more than one type of analyte.
compounds ionized by
UV radiation
sulfur or nitrogen
containing compounds
halogenated compounds
flame ionization
Answer Bank
mass spectrometer
nitrogen and phosphorous
containing compounds
thermal conductivity
electrolytic conductivity
hydrocarbons
electron capture
thermionic
photoionization
arrow_forward
Three students did a chromatography experiment, where Rf = distance of solute / distance of solvent.
What could be the possible errors why student 3 had results that are quite far from that of students A and B?
arrow_forward
use the following chromatogram and table as your data.
What is the %composition of the Vitamin A peak in the sample?
78.43%
77.73%
7.867%
72.74%
arrow_forward
PLEASE Answer within 20-30 minutes. Thank you!
arrow_forward
A student performed a chromatography
experiment to determine the Rf value of an
unknown amino acid. The distances traveled by
the amino acid and the solvent line were 3.0 cm
and 8.5 cm, respectively. What is the Rf value for
this amino acid?
0.3 cm
O 0.35
1.8
5.5
0.85 cm
0.65
O 2.8
arrow_forward
The spectroscopic data in the table is generated with five solutions of known concentration.
Concentration (M)
0.0133
m=
0.0266
0.0532
0.106
0.213
Absorbance
0.1271
What is the intercept of the linear regression line?
0.08531
0.5388
1.069
Use a spreadsheet program, such as Microsoft Excel, to graph the data points and determine the equation of the best-fit line.
1.954
What is the slope of the linear regression line formed by these points?
M-1
arrow_forward
Which is correct?
Random errors affect accuracy and systematic errors affect precision.
An accurate result shows agreement with the true or accepted value.
Precise results can contain significant random error, but little systematic error.
The standard deviation is a good measure of accuracy.
Polydimethylsiloxane (PDMS) stationary phases have low polarities. Which of the following statements is TRUE about gas chromatography using PDMS stationary phases?
The gas chromatography column is typically packed with silica microparticles.
An oven is used to control the column temperature because a constant temperature is always needed in gas chromatography
Octane (C8H18, boiling point: 126 ºC) is eluted later than dodecane (C12H26, boiling point: 216 ºC)
3-Pentanone ((C2H5)2CO, boiling point: 102 ºC) is eluted later than propanol (CH3CH2CH2OH, boiling point: 97 ºC)
arrow_forward
Question: Using a sample of aspirin for HPLC analysis. If the aspirin was contaminated (wet) with trace amounts of a solvent such as ethyl acetate, hexane, or water, would this contaminant be detected and appear as a peak in the HPLC chromatogram? Briefly explain based on the type of detector used in HPLC analysis and what type of compounds can be detected.
My Answer: The HPLC analysis detects chromophores, compounds with multiple double bonds. Since water, ethyl acetate, and hexane all lack multiple pi bonds this will not be detected in the HPLC.
-Question for you, is this complete (and correct), and should I include anything about UV detection? Is UV detection used in HPLC? I thought it was only used on TLC to show compunds that arent visable.
arrow_forward
How else can chromatography be used?
None of these answers are correct.
To wash (or remove) unwanted solvents from a mixture.
To identify or exclude components in a mixture.
To generate IUPAC names for hydrocarbons.
arrow_forward
Table 2: Absorbance Values of Standards and Unknowns.
Sample
Blank
Standard Solution 1
Standard Solution 2
Standard Solution 3
Standard Solution 4
Standard Solution 5
Water Sample 1
Water Sample 2
Phosphate Concentration
(in ppm)
Y17
0 ppm
0.02 ppm
0.04 ppm
0.08 ppm
0.16 ppm
0.32 ppm
Freeman Lake
0.001
0.325
0.292
0.413
0.315
0.039
0.054
0.049
Absorbance at
2= 690 nm
Calculations:
1. Construct a phosphate standard curve in Excel by plotting concentration (in ppm) on your
x-axis and Absorbance (unitless) on your y-axis for your known solutions. Label the axes
on the graph and provide the curve with a title. Use a linear trendline to generate a best fit
line to your data. Label the graph with the equation and the R" value. Insert your labeled
graph in the space below.
X
arrow_forward
The amount of caffeine in an analgesic tablet was determined by HPLC using a
normal calibration curve. Standard solutions of caffeine were prepared and analyzed
using a 10-µL fixed-volume injection loop. Results for the standards are summarized
in the following table.
Concentration of Standards
Signal
(arbitrary units)
(ppm)
50.0
8354
100.0
16925
150.0
25218
200.0
33584
250.0
42002
The sample was prepared by placing a single analgesic tablet in a small beaker and
adding 10 mL of methanol. After allowing the sample to dissolve, the contents of the
beaker, including the insoluble binder, were quantitatively transferred to a 25-mL
volumetric flask and diluted to volume with methanol. The sample was then filtered,
and a 1.00-mL aliquot was transferred to a 10-mL volumetric flask and diluted to
volume with methanol. When analyzed by HPLC, the signal for the caffeine was
found to be 21469. Report the number of milligrams of caffeine in the analgesic
tablet.
arrow_forward
Color BEFORE adding
Color AFTER adding
Intensity of color AFTER
adding FeCl3
Test Tube #
FeCl3
FeCl3
#1 (salicylic acid)
clear
lilac
100%
#2 (commercial aspirin A) clear
slightly pink
50%
#3 (commercial aspirin B) clear
lilac
95%
#4 (aspirin from your
clear
pink
20%
synthesis above)
#5 (control)
clear
clear
0%
Based on this data and the melting point of Aspirin, how pure is the synthesized product?
arrow_forward
What is the relative abundance of 35Cl in this sample?
42.9%, 57.1%, or 52.8%
arrow_forward
■ True or False: in a TOF analyzer, a fragment of low mass reaches the
detector first
▪ In GCMS when we know what analytes we are measuring we use
not know what analytes are in the sample we use
than scan.
▪ A) Scan; SIM
■ B)SIM; scan
• C) SIM; SIM
mode. When we do
mode. SIM allows greater sensitivity
arrow_forward
1.
Data & Results
4.0 mglmL
Concentration of stock solution (include units):
Table 1-Beer's Law Plot Data
V Volume of standard
solution dispensed (mL)
M, Diluted concentration
(include units)
Sample
Absorbance
A
2.00 mL
0.084
4.00 mL
0.173
l6.00 mL
0.259
8.00 mL
0.340
E
10.00 mL
0.418
Equation of trendline from Beer's Law plot:
whats the diluted concentration and equation of
trendline from the beers law plot once created?
arrow_forward
SEE MORE QUESTIONS
Recommended textbooks for you

Chemistry & Chemical Reactivity
Chemistry
ISBN:9781337399074
Author:John C. Kotz, Paul M. Treichel, John Townsend, David Treichel
Publisher:Cengage Learning

Chemical Principles in the Laboratory
Chemistry
ISBN:9781305264434
Author:Emil Slowinski, Wayne C. Wolsey, Robert Rossi
Publisher:Brooks Cole
Related Questions
- PART ONE: Preparation of FECNS* Solution Prepare the two solutions in table 1 by accurately measuring the required volumes of distilled water and CNS into two labeled test tubes. The absorbance of the solutions must be measured soon after adding the Fe" solution from a buret. TABLE 1 Fe 5 ml 5S mL Solution Distilled Water CNS 2 mL Total Volume 3 ml 2 ml 10 ml 3 mL 10 ml In this part of the experiment you are to explore what the above quote means. As a PRELIMINARY Exercise, formulate a hypothesis as to what would happen under each of the following circumstances: 1. 1ml of 0.0020 M CNS' solution is added to the unused portion of solution 1 in the test tube 2. One drop of 0.20 M CNS solution is added to the unused portion of solution 2 in the test tube.arrow_forwardHow does ammonia, dimethylglyoxime (DMG), and 8-hydroxyquinoline (8HQ) interact with your cations, leading to the evolution of colored species? Context: Paper chromatography experiment (separation of inorganic cations) with a 9:1 acetone/ HCl solventarrow_forwardIdentify the detectors in gas chromatography that can be used for the detection of each type of analyte. More than one detector may be appropriate for a given analyte, and a given detector may be appropriate for more than one type of analyte. compounds ionized by UV radiation sulfur or nitrogen containing compounds halogenated compounds flame ionization Answer Bank mass spectrometer nitrogen and phosphorous containing compounds thermal conductivity electrolytic conductivity hydrocarbons electron capture thermionic photoionizationarrow_forward
- Three students did a chromatography experiment, where Rf = distance of solute / distance of solvent. What could be the possible errors why student 3 had results that are quite far from that of students A and B?arrow_forwarduse the following chromatogram and table as your data. What is the %composition of the Vitamin A peak in the sample? 78.43% 77.73% 7.867% 72.74%arrow_forwardPLEASE Answer within 20-30 minutes. Thank you!arrow_forward
- A student performed a chromatography experiment to determine the Rf value of an unknown amino acid. The distances traveled by the amino acid and the solvent line were 3.0 cm and 8.5 cm, respectively. What is the Rf value for this amino acid? 0.3 cm O 0.35 1.8 5.5 0.85 cm 0.65 O 2.8arrow_forwardThe spectroscopic data in the table is generated with five solutions of known concentration. Concentration (M) 0.0133 m= 0.0266 0.0532 0.106 0.213 Absorbance 0.1271 What is the intercept of the linear regression line? 0.08531 0.5388 1.069 Use a spreadsheet program, such as Microsoft Excel, to graph the data points and determine the equation of the best-fit line. 1.954 What is the slope of the linear regression line formed by these points? M-1arrow_forwardWhich is correct? Random errors affect accuracy and systematic errors affect precision. An accurate result shows agreement with the true or accepted value. Precise results can contain significant random error, but little systematic error. The standard deviation is a good measure of accuracy. Polydimethylsiloxane (PDMS) stationary phases have low polarities. Which of the following statements is TRUE about gas chromatography using PDMS stationary phases? The gas chromatography column is typically packed with silica microparticles. An oven is used to control the column temperature because a constant temperature is always needed in gas chromatography Octane (C8H18, boiling point: 126 ºC) is eluted later than dodecane (C12H26, boiling point: 216 ºC) 3-Pentanone ((C2H5)2CO, boiling point: 102 ºC) is eluted later than propanol (CH3CH2CH2OH, boiling point: 97 ºC)arrow_forward
- Question: Using a sample of aspirin for HPLC analysis. If the aspirin was contaminated (wet) with trace amounts of a solvent such as ethyl acetate, hexane, or water, would this contaminant be detected and appear as a peak in the HPLC chromatogram? Briefly explain based on the type of detector used in HPLC analysis and what type of compounds can be detected. My Answer: The HPLC analysis detects chromophores, compounds with multiple double bonds. Since water, ethyl acetate, and hexane all lack multiple pi bonds this will not be detected in the HPLC. -Question for you, is this complete (and correct), and should I include anything about UV detection? Is UV detection used in HPLC? I thought it was only used on TLC to show compunds that arent visable.arrow_forwardHow else can chromatography be used? None of these answers are correct. To wash (or remove) unwanted solvents from a mixture. To identify or exclude components in a mixture. To generate IUPAC names for hydrocarbons.arrow_forwardTable 2: Absorbance Values of Standards and Unknowns. Sample Blank Standard Solution 1 Standard Solution 2 Standard Solution 3 Standard Solution 4 Standard Solution 5 Water Sample 1 Water Sample 2 Phosphate Concentration (in ppm) Y17 0 ppm 0.02 ppm 0.04 ppm 0.08 ppm 0.16 ppm 0.32 ppm Freeman Lake 0.001 0.325 0.292 0.413 0.315 0.039 0.054 0.049 Absorbance at 2= 690 nm Calculations: 1. Construct a phosphate standard curve in Excel by plotting concentration (in ppm) on your x-axis and Absorbance (unitless) on your y-axis for your known solutions. Label the axes on the graph and provide the curve with a title. Use a linear trendline to generate a best fit line to your data. Label the graph with the equation and the R" value. Insert your labeled graph in the space below. Xarrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
- Chemistry & Chemical ReactivityChemistryISBN:9781337399074Author:John C. Kotz, Paul M. Treichel, John Townsend, David TreichelPublisher:Cengage LearningChemical Principles in the LaboratoryChemistryISBN:9781305264434Author:Emil Slowinski, Wayne C. Wolsey, Robert RossiPublisher:Brooks Cole

Chemistry & Chemical Reactivity
Chemistry
ISBN:9781337399074
Author:John C. Kotz, Paul M. Treichel, John Townsend, David Treichel
Publisher:Cengage Learning

Chemical Principles in the Laboratory
Chemistry
ISBN:9781305264434
Author:Emil Slowinski, Wayne C. Wolsey, Robert Rossi
Publisher:Brooks Cole