95% ethanol (ethyl alcohol denatured) Shampoo Pantene Laundry detergent Sam’s Club Brand Dishwashing Liquid Great Value Brand Balance 3 200 mL graduated beakers 1 100 mL beaker 270 grams of kiwi Kitchen utensils 1 funnel Ice Filter paper and coffee filter paper Hot Water Two saucepans or other large containers Table salt 9 test tube Hot plate Eye dropper pH paper
DNA stands for deoxyribonucleic acid. DNA is the set of nongenetic traits, qualities, or features that characterize a person or other living thing. Extracting DNA helps understand different types of genes, as there are 500 different types of genes in total and counting. DNA can be found everywhere. In every cell, in every
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-Smriti Chopra
Liberating the DNA:
1. Prepare the ice bowl by putting ice and water into a pan about 5 to 8 cm deep. Put 50 mL of chilled ethanol into the 100mL beaker and place the beaker in the ice bowl.
2. Let’s start the DNA extraction solution. Put 90 mL of water in a 200 mL beaker and dissolve 2 grams of salt. Then add 10 mL of detergent and stir until mixed.
3. Get the kiwi and peel it, then cut it into chunks.
4. Measure 30 grams of strawberry chunks, then thoroughly mash the chunks properly.
5. The mashed strawberry must be placed in a 200-mL beaker.
6. 5 mL of the DNA extraction solution must be poured over the fruit.
7. Prepare a hot water bath by putting hot water into a saucepan about 5 to 8 cm deep. Check the temperature of the hot water bath. It must stay at a 50° C temperature.
8. Place the beaker with the strawberry and extraction solution into the hot water bowl. Let the fruit and extraction solution set in the hot water bath for 10 to 15 minutes. Stir the solution. The temperature of the water bath must be at 50°C at all times.
9. After 10 or 15 minutes, transfer the beaker containing the fruit and extraction solution mixture to the ice bath. Allow it to stay there for 5 minutes,
Use ice if you need to. Then, fill one beaker with 175 mL of water and the other with 350 mL. Warm the water in the 350mL beaker up to 55 degrees celsius and cool the water in the 175mL beaker to 15 degrees celsius, the same temperature as the pitcher because it will be your control group. Once the beaker that should be heated is at 55 degrees celsius, pour 175 mL of the water into a glove and pour the other 175 mL into a ziplock baggie. Pour the 15 degrees celsius, 175 mL of water into another ziplock baggie. Before you set these in water, have a stopwatch ready and make sure that the water in the baggies and glove is at the right temperature.
27. Heat the water to 40°C with an alcohol lamp by setting up the apparatus as shown in figure 3 below:
DNA is the genetic material that makes up the characteristics of all living organisms. While all human DNA is very similar in nature, there is just enough differences in
After the 5-minute period, take the Elodea and thermometer out of the beaker, pour the mixture into the beaker down the drain and rinse.
9) Trial E: Remove the syringe and empty the beaker. Add a Thermometer to the beaker. Add 200 mL of Room Temperature water to the beaker and heat with a Bunsen Burner until it reaches 100° C. Remove the Bunsen Burner. Repeat Steps 5 & 6.
Fill a test tube about 1/3 full with cold tap water for use in step 34.
DNA Profiling exists in blood, bone, hair follicles, saliva, semen, skin and sweat. They are the same in every cell and retain their distinctiveness throughout an individual’s lifetime.
Pour approximately 50 mL of room-temperature distilled water into the glass beaker by using the estimated volume on the beaker.
b. Place crushed ice in the beaker so the water level is just below the top of the
1) Separate the solid from the liquid in the beaker by decanting the liquid. Ask your instructor to demonstrate the correct procedure.
1.) Transfer the distillate to separatory funnel. Fluid didn’t seem very clear but sufficient to finish our lab on time.
Submerge the graduated cylinder in the plastic tub so that it is completely filled with water. Hold the open end of the graduated cylinder and move it vertically upside-down where the open end of the graduated cylinder is still submerged in the plastic tub. Clamp the graduated cylinder the ring stand of the lab table to keep it in place. perforate a hole in the top of the rubber cork for the solution container. Cut a straw the length of about four inches. place the straw inside of the rubber cork hole. Set up your timer for two minutes.
The boiling tubes will be used to hold the juice and the 1 % DCPIP solution. I will use boiling tubes because in the preliminary test I used a test tube and I found that the test tube was too small and was hard to shake the mixture therefore I will be using boiling tubes as the are slightly
2. Add about 20 mL of distilled water and stir the mixture with a glass stirring rod to dissolve the sample. There may be a small amount of insoluble residue. If your sample does not dissolve completely, remove the insoluble material by filtration.
3. Use a sterile pipette to transfer 0.1 ml of each dilution on to a MacConkey agar plate.