Acetaminophen Chromatography

The goal of this experiment was to find out active chemical components in Anacin and Tylenol, using Thin Layer Chromatography technique. This technique uses the difference in the intermolecular forcer and polarity to separate mixtures. Comparing Rf values were then used to determine the active chemical components in the two analgesics. The overall result was that Acetaminophen exists in Tylenol and Acetylsalicylic Acid exists in Anacin.

Thin Layer Chromatography (TLC) is an extremely useful technique for monitoring reactions. It is also used to determine the proper solvent system for performing separations using column chromatography. TLC uses a stationary phase, usually alumina or silica, that is highly polar (standard) or non-polar (reverse phase), and a mobile phase, some solvent whose polarity you will choose. In 5.301, and in most lab applications, you will use standard phase silica plates. You will apply your reaction mixture in solution to the plate then "run" the plate by allowing a solvent (or combination of solvents) to move up the plate by capillary action.

Adsorption chromatography occurs when “one substance form[s] some sort of bonds to the surface of another one”, creating intermolecular forces between the two substances (Chemguide). For thin layer chromatography, the components of the mixture are adsorbed onto the stationary phase that covers the plate (Chemguide). The more polar a substance is, the more strongly adsorbed it is (Chemguide) and the strong intermolecular forces then result in a slower rate of migration with respect to the moving phase. As the solvent touches the TLC plate, the solute (mixture) is allowed to move up the TLC plate

The isolation of aspirin, acetaminophen, and caffeine from Excedrin utilized the differing acidities and polarities of the three compounds. Extraction involved separating the three components by reacting them with HCL and NaOH, while thin layer chromatography involved separating the isolated compounds on a TLC plate. The binder was the first component extracted; followed by aspirin, acetaminophen, and caffeine was extracted last since it is a neutral and polar compound. The entire process can be seen in figure 1. The most utilized methods of extraction were gravity filtration and vacuum filtration which are displayed in figures 3 and 4 respectively. These methods were utilized to separate compounds based upon their differing

In the first part, there may have been cross contamination of materials, especially since periods before us had already performed the experiment. During chromatography, our spot sizes were initially very varied, as we didn’t know exactly how much of each substance to place when we began. Thus, the substances in more abundance would have greater opportunity to travel up with the eluent, skewing the results. In the beginning, we even put 6-7 drops, causing some of the substances to mix together. Touching the TLC plate may also have disrupted some of the substances traveling up.

Thin Layer Chromatography (TLC) works in relation to the polarity of chemicals. A plate is first covered with aluminum foil or silica etc. and has solutions of varying polarities placed upon the aluminum foil or silica. When placed in a in a puddle of solvent that moves up the plate, the different inks i.e. the solution will move up the place based on their Rf values. Adherence increases with increasing polarity, so the less polar compounds will be carried farther by the solvent. Eventually the dyes will separate into their compontnets, which can be visibly seen. This is then used to determine who the ink of the unsigned note belonged to along with the pen that it belonged

0.300 grams of biphenyl/ p-toluidine sample was weighed. Next, 10 mL of dichloromethane was measured in a graduated cylinder. The dichloromethane was transferred to a small beaker then the solid mixture was dissolved in it. A Thin Layer Chromatography (TLC) was conducted with the dissolved mixture in 20% Ethyl Acetate and 80% Hexane solution. The TLC plate was observed to be impure with two spots. To being extraction, a separatory funnel was placed inside of the hood and the stopcock was closed. A flask was placed under the funnel then the mixture was added to the funnel. Next, 10 mL of 3M HCL was measured in a graduated cylinder and

On a thin chromatography plate, five spots were placed ( as shown in table 2) and the plate was developed using chloroform/methanol. This was later visualized with dragendorff’s reagent under the UV light. All separated components were observed, identified and recorded.

Results: There were different food dyes, solutions, beakers, chromatography strips, Erlenmeyer flasks, tooth picks, and watch glasses on the lab tables. There was also a ruler and a pencil. The first thing I did was prepare two strips of chromatography paper as instructed in the procedures. Next I used a toothpick

Aim The objective of this experiment was to separate a mixture of two compounds (ferrocene and acetylferrocene) using adsorption column chromatography. Introduction Adsorption column chromatography is a technique that uses a solid stationary phase which is fixed and a liquid mobile phase that moves slowly through the packed column. This technique uses the property of polarity as the compounds have a greater affinity towards the respective phases (either stationary or mobile) which leads the compounds moving at different rates through the packed column and separating from one another.

The purpose of this experiment was to learn about the analytical method known as chromatography which allows the separation of a mixture into its molecular components. In order to illustrate this technique the pigments found in the leaves of spinach were separated by means of thin layer chromatography (TLC). All chromatographic techniques have one principle in common; a liquid or gaseous solution of sample, called the moving phase, is passed (moved) through an adsorbent, called a stationary phase. With TLC the adsorbent of choice was one of the most common coatings, silica gel, SiO2. This compound is polar, and therefore, is frequently used in the separation of polar substances such as aldehydes, ketones, amines and carboxylic acids.

In this experiment, the technique of thin layer chromatography is used to identify the identity of unknown. To aid in seeing the spots from the thin layer chromatography, UV light and iodine adsorption is used.

Chromatography Investigation Chromatography is a highly regarded technique used to separate the components of a mixture. It is based on the principle that each component possesses a unique affinity for a stationary phase and a mobile phase. The components that are more inclined to enter the mobile phase will migrate further on the chromatogram and distinguish themselves from the other components. The type of solvent used in chromatography is known to directly affect the separation of the mixture. In this experiment, thin-layer and column chromatography will be utilized to separate the numerous chlorophyll and carotenoid pigments of a spinach extract.

During this experiment the crude product (mixture of ortho and para nitrophenols) was run through a column chromatography. The point of the column chromatography was to separate the nitrophenols and purify them. During the experiment the crude product was ran through the silica with two different solvents. 60:40 DCM/hexanes was used to form the first band while 50:50 DCM/EtOAc was used to form the second band. The reason for this method was that there had to be a change in solvent for the para product because since it is polar it was expected to run very slowly through the column and therefore needed a more polar solvent to speed it up, ethyl acetate. As expected two different yellow bands were observed. Each band represented one of the nitrophenols, with the bottom band being the ortho-nitrophenol since it is less polar and stayed within the solvent and therefore ran through the column quickly. The para product stayed at the top because it was similarly polar to the Silica and had the ability to hydrogen bond to it. Once the bands were formed fractions were taken from the yellow bands. Fraction 1-5 was from the ortho product and fractions 6-10 were from the para product. The fractions formed were than ran through TLC chromatography to test the true purity of the products and the success of the column chromatography separation. The first TLC plate containing fractions 1-5, surprisingly did not have any spots (Figure 1b). This is was unexpected because fractions 1-5 were

AIM : Thin-Layer Chromatography can show many different characteristics of a mixture. It is recognized for isolation , separation ,identification, and anaylsis of the mixture’s components. The purpose of this experiment is to separate carbohydrates into its pure components such as mixtures of monosacrides by TLC. TLC is used to identify sugars in normal and pancreatic disease urine, the procedure is easy and reproducible .