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Allura Red In Red Gatorade Essay

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Abstract: The concentration of Allura Red in red Gatorade was determined. It was done by using the equation M1V1 = M2V2 and comparing the concentration of the diluted solution to find concentration of the undiluted concentration. The concentration of the Allura Red in Gatorade was 1.238*10^-4 M. Introduction: The goal or purpose of this experiment was to determine the concentration of Allura Red in red commercially available beverage- Gatorade. Colorimeter are used to shine a LED light through the solution and hit a photocell: it will detect an absorbance or a percent transmittance value. These “value” can be charted and examined as a calibration curve. Calibration curve is a method for determining a substance concentration in an unknown sample …show more content…

Each stock solution was placed in a colorimeter and was tested for it Absorbance. A computer program tested and drew up the Calibration curve/linear fit equation. However, the computer could not protect potential errors. An error for determining the concentration of the diluted and undiluted, could be a skew linear fit equation. The linear fit equation could be skewed by having an inadequate ratio of the stock solution and distilled water. For example, when making stock solution 1, 0.021(L) was used instead of 0.020(L) can throw the calibration curve, resulting a skewed linear fit equation. If the “blank” was not fully clean or had left over Allura Red residue, then the “blank” was tampered with. A tampered “blank” means any comparisons with it would have a wrong Absorbance reading. However, the most likely and most effective error, is calculation. Using the wrong V1 and V2 to determine the concentration of the undiluted would affect the answer of the grams Allura Red would be consume and the amount of molecules of Allura Red. The colorimeter is adjusted to a wavelength of 470 nm is maximize the absorbance of the Allura Red. If wavelength was place at 565 nm, then Allura Red would not absorb as much color of

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