Abstract: The concentration of Allura Red in red Gatorade was determined. It was done by using the equation M1V1 = M2V2 and comparing the concentration of the diluted solution to find concentration of the undiluted concentration. The concentration of the Allura Red in Gatorade was 1.238*10^-4 M. Introduction: The goal or purpose of this experiment was to determine the concentration of Allura Red in red commercially available beverage- Gatorade. Colorimeter are used to shine a LED light through the solution and hit a photocell: it will detect an absorbance or a percent transmittance value. These “value” can be charted and examined as a calibration curve. Calibration curve is a method for determining a substance concentration in an unknown sample …show more content…
Each stock solution was placed in a colorimeter and was tested for it Absorbance. A computer program tested and drew up the Calibration curve/linear fit equation. However, the computer could not protect potential errors. An error for determining the concentration of the diluted and undiluted, could be a skew linear fit equation. The linear fit equation could be skewed by having an inadequate ratio of the stock solution and distilled water. For example, when making stock solution 1, 0.021(L) was used instead of 0.020(L) can throw the calibration curve, resulting a skewed linear fit equation. If the “blank” was not fully clean or had left over Allura Red residue, then the “blank” was tampered with. A tampered “blank” means any comparisons with it would have a wrong Absorbance reading. However, the most likely and most effective error, is calculation. Using the wrong V1 and V2 to determine the concentration of the undiluted would affect the answer of the grams Allura Red would be consume and the amount of molecules of Allura Red. The colorimeter is adjusted to a wavelength of 470 nm is maximize the absorbance of the Allura Red. If wavelength was place at 565 nm, then Allura Red would not absorb as much color of
In addition to their respective traditional beverages, both Powerade and Gatorade have a wide range of specialty flavors and mixtures. For instance, Gatorade features low-calorie beverages with only 7 grams of sugar per serving, as well as special drinks for endurance athletes with 300 milligrams of sodium and 140 milligrams of potassium. Powerade features beverages that have zero calories and that offer electrolytes and vitamins but zero grams of sugar.
After the serial dilutions of the red and blue dyes were taken, the molarity and absorbance for both dyes were calculated. Using the MiVi = MfVf equation, the concentrations for each value of the red and blue dye were separately calculated. Calculating absorbances calls for setting the correct wavelengths of light for each dye. In this case, the 470 nm wavelength for red dye and the 635 nm wavelength for blue dye was needed to find the maximum absorbances. The absorbance was found by blanking the colorimeter and entering the concentrations. After both values of the absorbances and concentrations were found, the values were then graphed in order to obtain the equation of the relationship between absorbance and concentration.
After this, the solution was poured into a volumetric flask just about to the 1dm3 line and then it was left there to cool to the same temperature as the room before filling precisely to the 1dm3 line with distilled water. The molar mass of CuSO4.5H20 was 249.5 so that means 249.5g of copper sulphate was needed to dissolve, in order to make a standard solution, into 1dm3of distilled water. Following this, a linear dilution of the CuSO4.5H2O was made in order to be used to make a calibration curve after using the colorimeter to write down the absorbance of each sample. A linear dilution is diluted with distilled water in order for it to make the concentration weaker and weaker. For this investigation, the dilutions made ranged from 0.01 to 0.1 M/l . It was essential to only make up 10cm3
An experiment was done to determine the concentration of Allura Red in a commercially available beverage, Gatorade. This was done by using the colorimeter, the absorbance of five Allura Red solutions were measured. The graph obtained demonstrated a linear relationship and the equation obtained yielded a concentration of 1.9910-6M.
The Beers Law calibration experiment used many concentrations of crystal violet solutions. Each of these solutions were test and analyzed in order to determine the absorbance of each concentration The results were than graphed and produced a slope of 1.00E05 with an intercept of -2.21E-02.
Make sure to use the same type of cuvette to keep the width consistent and to prevent any experimental error from arising. Obtain 5 of the same type of cuvettes and pre-rinse them thoroughly. Label them numbers one through five in increasing molarity. Then, fill each of the cuvettes with one of the five solutions you created back in Part A. We will first examine the solution that exhibits the highest concentration or molarity. Make sure to wipe the outside of the cuvette with a Kimwipe before placing into the SpectroVis Plus device. Observe the graph that is generated and make sure to take note where the maximum absorbance takes place.
The values of color absorbance are effective because color absorbance has a linear relationship with concentration values, which in turn, allows us to easily find concentration values for many solutions. Beer’s law describes this phenomenon since the absorbance is directly proportional to concentration. We observed that as the color absorbance increased, the concentration of the FeSCN2+ complex ion increased. This is because as the FeSCN2+ concentration increases, the blood-red color becomes darker due to more presence of the blood-red FeSCN2+ ion. Therefore, the color absorbance increases because there is more blue color absorbed by the darker red color. We then graphed the absorbance and concentration values and created a line of best fit. Using the line of best fit, we were able to predict the equilibrium concentrations of the FeSCN2+ solutions and find the change required to reach equilibrium. Since we already knew the initial concentration of FeSCN2+ and since we already found the equilibrium concentration of FeSCN2+, we can calculate the change in equilibrium. Using this data, we were able to calculate the equilibrium concentration of all of the species in this lab, since we already knew the change from the initial concentration to the equilibrium change. Q is less than K because there was no initial concentration of FeSCN2+, but after the system reached
The concentration of sugars in two well-known sports drinks, Powerade and Gatorade, were determined by monitoring an enzyme-catalysed reaction sequence involving the appearance of NADPH. Sucrose and glucose concentrations were calculated from the concentration of NADPH formed by the reaction of glucose-6-phosphate and NADP+. Spectrophotometric absorbance readings were taken at 340nm, this is because NADPH absorbs strongly at this wavelength, whilst NADP+ does not (1015MSC, 2010). The concentration of glucose and sucrose in
Gatorade is one of the most famous sports drinks on the market today. Ever since it was created in 1965 at in the labs of the University of Florida Gatorade was used to hydrate in drastic weather conditions. Also Gatorade was used to replenish the key nutrients lost during playing. This achievement was proven to work when the Florida Gators won the 1966 Orange Bowl over the Georgia Tech Yellow Jackets. Today, Gatorade has become the largest sports drink company of all time being the official sports drink of the NFL, AVP, PG, MLB, MLS, and many other
What effect does Gatorade have on your teeth? If the Gatorade is red, it will have a Gatorade effect then the yellow Gatorade would have. First, set five plastic cups out in a set area that will not change. Next, fill each cup up with 118.294 Ml of a color of Gatorade or water. There will be one color of each Gatorade and one cup of water during each trial. Set one egg in each cup. Then leave the eggs in the Gatorade or water over night (about 12 hours). The next morning remove each other the eggs and place them one a plate. Measure the color of each of the eggs using the flawless teeth whitening scale, or the color scale correlated with the color of Gatorade. The red Gatorade stain the most, then blue, then orange, then yellow, the water
The purpose of this lab was to investigate the amount of sugar in carbonated beverages by using the density of solutions, and also to create a standard curve with the percent sugar in sugar solutions.
Each solution contained different concentrations as follows: 0.005 mg/mL, 0.010 mg/mL, 0.015 mg/mL, 0.020 mg/mL, and 0.025 mg/mL. Each solution needed to have a volume of 10 mL. Before adding the different concentrations of Coomassie Blue into their separate tubes, the formula C1V1= C2V2 was used in order to determine how much stock solution is needed for the five dilute solutions. Once that number was calculated, a pipette was used to add the amount of stock solution needed for each tube. We then subtracted the amount of stock solution from 10 mL to determine the amount of H2O needed. The calculated amount of H2O was then added to each tube of solution. After doing that, a spectrophotometer was used to determine each solution’s relative absorbance. However, before that, we first had to calibrate the spectrophotometer before determining each solution’s relative absorbance. In order to calibrate the spectrophotometer, a disposable culture tube filled with distilled water was used. We then changed the data rate to 100 and removed the tube with water. In order to determine the relative absorbance, the relative absorbance had to be at 595 nm. Also, during this experiment, an unknown dilution was given to us by the lab instructor. We determined the relative absorbance by using the spectrophotometer and then recorded the results. The procedures for this experiment can be found on page 8 of
Gatorade was named after the university’s athletic team, The Gators. Gatorade was used to rehydrate and give additional energy to the athlete’s after an active day at practice.
Decades ago, when hummingbird feeders were made from plain glass vials or bottles, a little red coloring was often added to the sugar water to attract the birds’ attention. A tradition was born, and though modern commercial feeders are proven attractive without additional color in the contents, to many people a hummingbird feeder just doesn’t look right unless it’s filled with red liquid.
The color red is more than just a color. Certain colors can revoke emotions and feeling. For example, black symbolizes darkness, sadness, and emptiness. Blue illustrates sadness and loneliness. Red, however, is a representation of passion, love, and ander. It is the strong passion that is felt by people doing the things that they love. Red is the feeling that people get when they see their loved ones. It is the anger and jealousy felt . Red is more than just a color, it is a feeling.