Brine Shrimp Project Hypothesis

AP Biology Lab Report: How the Amount of Motor Oil Affects Hatching Viability of Brine Shrimp Eggs

Firstly adult female brine shrimp ovulate approximately every 140 hours and in optimal conditions the fertilised eggs can almost immediately hatch, even over a relatively short lifetime of a year this is a lot of young. They have another brilliant reproductive adaption; while in extreme conditions, such as low oxygen level or salinity above 15‰, female brine shrimp produce eggs with a chorion coating which has a brown colour. These eggs, also known as cysts, are metabolically inactive and can remain in total stasis for two years while in dry oxygen-free conditions, even at temperatures below freezing. The shrimp have also been able to survive dangerous salinity levels as the eggs wait to hatch if the salinity levels are too high. Once placed back in briny water the eggs hatch within a few hours. This ability also has enabled them to spread the population by the eggs travelling on the feet of birds. They have also been known to reproduce asexually by parthenogenesis in the absence of

Test tube four contained a snail and was placed in the dark. It was hypothesized that the water would remain yellow after a day.

Brine Shrimp are a micro-crustacean that is found mostly in inland saltwater bodies, however, the salinity levels that they live in vary greatly. The conditions that they require can be made at home as the eggs (cysts) of the shrimp are dry, hard shells that can withstand drought and go without water for up to 50 years. This study presents the results of 3 different salinity levelled environments to the Brine shrimp in order to discover a salinity level that produces the most successful hatching rate. By filling 3 Petri dishes with 20mL’s of purified water, each dish contained varying sodium chloride amounts from 15ppt, 25ppt, and

One of the limitation was that the experiment was unable to simulate the effects of predation. In an actual environment some of the brine shrimp eggs could have been eaten before they are able to hatch, but since this was in a petri dish there was no such thing as predators that have reduced the hatching viability of the brine shrimp eggs. Another, limitation was the exact amount of brine shrimps you could brush up. This made the hatching viability to not be as accurate because there was different amount of eggs to start with. If there was an equal amount of eggs to begin with the hatching viability percentage would have been more accurate since they all started the same. These two limitations made the experiment not as reasonable because it stopped what could have happen to these brine shrimps in their actual

This result was consistent with the other groups who tested out this question. They also got that the pill bugs preferred a dark environment. Furthermore, the data supports many studies that state that pill bugs prefer a dark and moist environment because that best replicates their natural habitat and provides for their needs. For example, one website claims that pill bugs will prefer the darkness and encourages the reader to test it. Another does multiple experiments on the pills bugs and in their experiment the pill bugs prefer the dark environment. After learning that pill bugs do not prefer light areas, I would want to test if there are other colored lights that attract them instead. An experiment can be done by keeping one chamber dark and using different colored lights such as red, green, or blue on the other side to see if it attracts any of the pill

The ocean floor is a very dark and is filled with algae however, crabs that live here can still easily catch and eat plankton. Scientists are wondering, is it the crab’s other senses doing the job or is it just there sight? On the ocean floor not much light gets in which leads everyone to think that the crabs can't see however, some light does reach the crabs and the plankton the crabs eat. Most visible light does not reach the ocean floor in normal conditions except yellow light and green light. Since the plankton reflects all visible light the plankton would appear yellowish green to the crab’s eyes. Even though the green and yellow light reaches the floor in normal conditions the area where the crabs live is packed with algae. According to evidence cards F and G algae reflects green light but all the other colors of visible light are absorbed with algae. This states that only green light has a large chance of making it to the bottom so the crabs can see the plankton.

The purpose of this lab was to answer the question, “Of the saline solutions of 0%, 0.5%, 1%, 1.5%, and 2% sodium chloride (NaCl), which solution will yield the highest hatching viability for the brine shrimp?” The hypothesis was that the saline solution most precise to the concentration of NaCl in seawater, which is approximately 3.5%, will yield the highest hatching viability. Therefore, the 2% NaCl solution will yield the highest hatching viability.

Brine Shrimp start out at something called cysts which you can buy all over the world and it takes only one night in water for them to hatch into the Brine Shrimp. At a maximum length, Brine Shrimp only get up to a little over 1 centimeter which is perfect size for fish around 10 centimeters. I thought that growing the Brine Shrimp was one of the most interesting things that I did while I was there.

Temperature had a direct effect on oxygen consumption of crayfish, Orconectes propinquus. Crayfish acclimated to warm temperature (20 to 25 C) had a mean mass of 8.25g +/- 1.05. Crayfish acclimated to cold temperature (3 to 5 C) had a mean mass of 10.61g +/- 0.77. Oxygen consumption rates of 30-60 minute treatments were used and there was no significant difference between the two different treatments (t=0.48, df=58, P=0.70). The data from 0-30 minutes were not used because the crayfish were disrupted by transportation and the data were not normally distributed. The Q10 value was 1.05, representing that there was full compensation for oxygen consumption for the crayfish at two different acclimated temperatures. The oxygen consumption of crayfish was not affected significantly by two different temperatures (Figure 1).

Referring to the experiment`s hypotheses that the A. franciscana prefers light, temperatures between 20-24 ̊ C, and a basic (pH 8) environment; the results regarding the first treatment, light, were initially vague. According to the experiment results, the A. franciscana did not show a clear preference towards light or dark because both sections contained high concentrations of them; the A. franciscana also strayed from the uncovered section. Several factors may shed light on the results such as the A. franciscanas physical appearance; they possess three light-sensitive eyes that can adjust to both low and high light intensities (Fox, 2001). This means that although they may prefer light they can survive in darker habitats as well; relating back to the experiment the A. franciscana may have been content with wherever they were, resulting in limited movement.

Behavior of Brine Shrimp in Habitat Selection Introduction: This experiment was developed primarily to find the optimal or preferred living conditions of adult brine shrimp. In this lab 3 variables were tested in different degrees to determine which the shrimp prefer. The different variables were light, ph, and temperature. From outside resources I have found that brine shrimp can survive in temperatures ranging from 4° Celsius to 40° Celsius with an optimal temperature of 20°-25°. So I hope to reinforce this research by testing its validity in the lab. From another outside source brine shrimp display positive photo taxis, which means that they are attracted to light. And finally from my last outside source I have found information that

Reaching a length of up to 5.5 centimeters (or about 2 inches), the bigclaw snapping shrimp is the largest member of the family Alpheidae, which are also known as the pistol shrimp. It is found in water up to 30 meters (98 feet) deep from as far north as North Carolina’s Outer Banks to the Brazilian coast. It also is able to survive in areas of rapidly different salinity; A. heterochaelis has be found in environments that range from mesohaline (having a salinity of 5 to 18 parts per thousand) to hyperhaline (having a salinity of 40+ ppt). This means that it lives in environments that range from brackish to seawater of above average salinity. It is most commonly found in areas where cover can be easily found during the day, such as an oyster reef.

The development of A. salina will be unfavorable if exposed to too much ethyl alcohol. Treatment two and three have more ethanol alcohol than treatment one and more brine shrimp died when put into more ethanol alcohol. Since the viability was less than the viability in treatment one, the hypothesis was supported. Treatment four was the constant and had no ethanol alcohol which less cysts died when compared to treatment two and three, but when compared to treatment one, more had died,

SnoLab is studying these effects on various organisms in two phases. The first phase is the effects of radiation absence on lake whitefish embryos. They chose these embryos because of their development period and the easy nature of raising them. The embryos are kept in two fridges of different temperature both underground and above