Causes Of Biofilm Formation

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The primary investigators of the research study include Sana S. Dastgheyb, Amer E. Villaruz, Katherine Y. Le, Vee Y. Tan, Anthony C. Duong, Som S. Chatterjee, Gordon Y. C. Cheung, Hwang-Soo Joo, Noreen J. Hickok and Michael Otto. The locations at which the studies were conducted include the institutions that each author is associated with. Those include the National Institute of Allergy and Infectious Diseases at Bethesda, Maryland and the Thomas Jefferson University located in Philadelphia, Pennsylvania. 2. A. What is the overall purpose of this scientific investigation? The researchers of this study are interested in exploring the factors that promote biofilm formation in human synovial fluid…show more content…
Bacterial aggregates were monitored using UV-fluorescent imaging of ethidium bromide-labeled bacteria. Thereafter, aggregate size distribution was measured using a T3 Cellometer. In addition, qRT-PCR was utilized for the amplification of the LAC strains, while luciferase reporter gene fusion constructs were made via plasmid amplifications. Polysaccharide intercellular adhesion (PIA) detection from strain LAC was achieved with immunoblotting techniques, which were previously used in analogous studies. The researchers also utilized scanning electron microscopy and biofilm assays that aided in the biofilm formation analysis. PSMs were quantified by reversed-phase high-liquid chromatography/electrospray mass spectrometry and finally statistical analysis was performed using the GraphPad Prism version 6.02 program. C. What are the general results/conclusion from the study? When LAC strain bacteria were introduced in the SF of noninfected patients, biofilm and aggregate formations appeared within a few hours. It was interesting to observe that even with consistent antibiotic treatment, the bacteria showed resistance and were able to form aggregates in less than a two-day period. Specifically, after incubation of the SF, aggregates appeared within the first few minutes. In the second set of results, the expression of fibronectin-binding proteins and fibrinogen-binding proteins was
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