Cellular Respiration Lab Report

The first lab was conducted to analyze how germination affects the rate of cellular respiration in lima beans compared to dormant seeds. In order to

There are many procedures during this lab and many materials needed for an accurate analysis of data. First, fill a 100 mL graduated cylinder with 50 mL of water. Add 25 germinating peas and determine the amount of water that is displaced. Record this volume of the 25 germinating peas, then remove the peas and put those peas on a paper towel. They will be used for the first respirometer. Next, refill the graduated cylinder with 50 mL of water and add 25 non-germinating peas to it. Add glass beads to the graduated cylinder until the volume is the same to that of germinating peas. Remove the beads and peas and put on a paper towel. They will be used in respirometer 2. Now, the graduated cylinder was filled once again, determine how many glass beads will be require to reach the same volume of the germinating peas. Remove the beads and they will be used in respirometer 3. Then repeat the procedures used above to prepare a second set of germinating peas, dry peas and beads, and beads to be used in respirometers 4,5,and 6, the only difference is the temperature of the water.

My lab partners and I performed an experiment that involved placing spinach disks into separate cups of distilled water (dH2O) and 0.2% sodium bicarbonate (NaHCO3) solution to examine photosynthesis in leaf tissue (Department of EEB, 2015). Discovering that the spinach disks quickly floated to the top of the 0.2% NaHCO3 solution and not in dH2O, we wondered if varied concentrations of carbonation would affect the rate of photosynthesis (PS). We tested this by halving the 0.2% NaHCO3 solution (using equal parts dH2O and 0.2% NaHCO3 solution to make 0.1% NaHCO3 solution). I hypothesize that if the spinach disks are placed in the 0.1% NaHCO3 solution, then they will have a slower PS compared to the disks placed in 0.2% NaHCO3. CO2

In this lab we are measuring the amount of oxygen used in both germinating and non germinating peas. We are measuring the oxygen consumption by taking a reading of a respirometer submerged in two water baths. The first bath will be cold water and the second warm to determine the effect of temperatures on oxygen consumption. Our negative control will be glass beads to measure to increase or decrease in atmospheric pressure or temperature changes. There is a direct relationship between oxygen consumption and Carbon Dioxide produced, therefore the more O2 consumed the more CO2 produced. To keep the amount of CO2 produced from canceling out any pressure gained or lost from the consumption of

The chickpeas in the hottest temperature tested produced the most amount of carbon dioxide meaning that the cellular respiration rate of the chickpeas was increased. The coldest temperature tested also produced the least amount of carbon dioxide meaning cellular respiration decreased in those chickpeas. For the most part the experiment ran more smoothly than expected. A few time during testing the test chamber was knock over but these accident had a minimal effect on the final outcome. There were sudden jumps when testing the runs in Figure 1, but the jumps were never major enough to cause a retrial. These jumps can be attributed to the fact that the carbon dioxide production was taken every 10 seconds in the 240 second experiment so anything outside disturbance during that time was reflected in the data. Overall the data completely supports the hypothesis. The 42°C run produced the highest slope, the 5°C run produced the lowest slope, and the room temperature (22°) run was in between the two other slopes. The 5°C run did have the most amount of ppm’s recorded, but this means nothing in the long run since the slope was still the lowest meaning it produced the least amount of carbon dioxide over the 4 minutes. The results obtained corresponded with other students in the class who also tested the relationship between cellular respiration and temperature as well as the studies discussed in the introduction

At standard temperature and pressure, the rate of aerobic respiration of the boiled peas is almost negligible compared to that of the germinating peas.

This experiment consisted of 3 respirometers, one with ants, one with radish seeds, and one with glass beads. Each with 4 pellets of KOH and a piece of cotton. They were placed in a water bath that was at 75 degrees fahrenheit. A bubble at the end of the respirometer was measured every five minutes, and this distance showed how well the organisms were respiring. The radish seeds were able to do the most cellular respiration in 25 minutes, with the ants being a close second, and the control respirometer of the glass beads doing the least.

The CO2 production was measured with a CO2 meter (+/- 1ppm), in order to determine the rate of anaerobic cell respiration in yeast.

Purpose: The purpose of this lab was to determine how light affected the amount of oxygen gas produced over time. The amount of oxygen gas consumed during cellular respiration and produced during photosynthesis was measured. The experiment also determined the rate of respiration and photosynthesis of a plant.

This lab deals with the transpiration rates in plants, specifically a tomato plant that was used for this experiment. Transpiration is when water leaves a plant through the stomata as water vapor while the stomata is capturing CO2 for photosynthesis. This experiment used three different scenarios: a tomato plant with a light shining on it, a tomato plant with wind blowing on it from a fan, and lastly a tomato plant with nothing acting on it. The hypothesis is that the rate of transpiration will be fastest with light, faster with wind, and slow with the control. This hypothesis was rejected because the rate of transpiration is as follows with the wind having the fastest rate: with light the rate was 7.60 mm/min, with wind 10.20 mm/min, and control 4.33 mm/min. The cause of the wind having a faster transpiration rate than the light may have been due to the surface area of the leaves on the tomato plants. The surface area of the leaves for the wind experiment is 8,124mm2, and for the light is 7,740mm2.By doing this transpiration experiment it helps one to see what happens in plants daily and understand why it happens.

Aerobic respiration is the way toward delivering cellular energy involving oxygen. Cells separate the food in the mitochondria in a long, multistep prepare that produces 32 ATP. The initial phase in is glycolysis, the second is the citric acid cycle and the third is the electron transport system. Germination is one of such procedures that make use of aerobic and in light of the fact that specific variables can influence aerobic respiration, it would likewise influence germination. The chemical reaction for aerobic respiration is:

All living organisms need the energy to perform the basic life functions. Cells use a process called cellular respiration to obtain the energy needed. In cellular respiration, cells convert energy molecules like starch or glucose into a cellular energy called Adenosine triphosphate(ATP). There are two types of cellular respiration which include: Aerobic and Anaerobic respiration. In aerobic respiration, cells will break down glucose to release a maximum amount of ATP this takes place in the presence of oxygen. Aerobic also produces carbon dioxide and water as waste products and it takes place in the mitochondria. on the other hand, anaerobic respiration, a metabolic process, also produces energy and uses glucose, but it releases less energy and does not require the

Each of the four different activity levels were tested three times. The same person performed all of the different runs in order to stay as consistent as possible. Nicole performed each activity for 2 minutes and then breathed into CO2 chamber with a straw to measure CO2 rates. See Lab 5 Metabolism II Biology 152, 2018 for set up of CO2 chamber and probe. The CO2 rates were tested to determine cellular respiration rate because as earlier stated, CO2 is a byproduct of this reaction, and therefore can be measured to determine the rate.

There are two types of cellular respiration, aerobic and anaerobic. Aerobic respiration occurs when there is oxygen present and in the mitochondria (in eukaryotic cells) and the cytoplasm (in prokaryotic cells). Aerobic respiration requires oxygen; it proceeds through the Krebs cycle. The Krebs cycle is a cycle of producing carbon dioxide and water as waste products, and converting ADP to thirty-four ATPs. Anaerobic respiration is known as a process called fermentation. It occurs in the cytoplasm and molecules do not enter the mitochondria for further breakdown. This process helps to produce alcohol in yeast and plants, and lactate in animals. Only two ATPs are produced through this process. In yeast fermentation is used to make beer, wine, and whiskey.

At the end of our experiment sucrose was actually the winner of the most carbon dioxide however; DR.