Lab Report 4: Protein Purification: Size Exclusion Chromatography Badrun Nessa Rahman Lab Partner: Briana Tolbert Section 55 Objective: The purpose of today’s laboratory is to understand how size exclusion chromatography plays a role in purifying protein which is an important concept to understand as it helps answer the mysteries which lay behind proteins such as
COLUMN AND THIN LAYER CHROMATOGRAPHY Maria Janine B. Abarientos, Kuia B. Allarde, Aliana Keshia P. Andino Mary Viadelle E. Andrada and Nina Marian Robelea G. Ang Group 1 2C Pharmacy Organic Chemistry Laboratory ABSTRACT In this experiment, the techniques column and thin layer chromatography was used to separate and determine the purity of the colored components of siling labuyo and malunggay leaves. The results obtained relied on differential solubilities and adsorptivities of the components to
INTRODUCTION OF CHROMATOGRAPHY Chromatography is discovered by Russian–Italian botanist Mikhail Semyonovich Tswett at the commencement of the 20th century, it is a physicochemical process for partition of composite mixtures [1]. In his paper ‘On a new category of adsorption phenomena and its application to biochemical analysis’ presented on 21st March, 1903 in frequent meeting of the biology section of the Warsaw Society of Natural Sciences, Tswett gave a very detailed report of the newly discovered
The detection and/or identification of trace amounts of explosives. The review of each method should include a description of the way the method works and the advantages and drawbacks of the technique. Introduction An explosive, are reactive substances that contain a large amount of potential energy that can yield an explosion if they are released rapidly, they are accompanied by the production of light, heat, sound, and pressure. Mainly used in the mining industry for extraction of minerals
investigating samples of polluted ground water will want to know which toxic ions might be present in a sample. Chromatography is one of the first tools used in such situations. In this technique, many types of mixtures can be separated into the component pure substances; by comparison to a standard sample, each component substance can also be tentatively identified. Many varieties of chromatography exist, each one designed to separate specific types of mixtures. The common feature of each type of
are made in laboratories by people who are unskilled and untrained. Urine There are two steps for testing of drugs. One is screening and the other is confirmatory test. Screening test is used to find the presence of drug or to identify a specific drug. The screening test for drugs are cheap and less time consuming. But the drawback of this test is not precise as confirmatory test. For detection of drugs in urine, there is two screening methods such as immunoassay and chromatography. Urine samples
Abstract Pigments extracted from different greens have different polarities and may be different colors. Mixed pigments can be separated using chromatography paper. Chromatography paper is able to separate mixed pigments due to their polarity and solubility. Pigments of chlorophyll a, chlorophyll b and beta carotene will be separated on chromatography paper because each has its own polarity and solubility, which results in different distance traveled up the paper. Beta carotene is non-polar so it
COLUMN AND THIN LAYER CHROMATOGRAPHY Mark Paul P. Pastrana, Mariah Ericka M. Patawaran, Princess Juneire M. Peligro, Francisco Q. Pua III, Rose Anne L. Quyo and Janille P. Ragpa Group 8 2B Medical Technology Organic Chemistry Laboratory ABSTRACT The main objectives were to separate the colored components of malunggay leaves by means of column chromatography, as well as to determine the purity of the components using thin layer chromatography (TLC) and measure the Rf values of the colored
Food color is added to many of the foods we eat. Food color are added dyes, pigments or substances that give color when used in a food, cosmetics, drugs, or the human body. In the United States all color additives are controlled by the Food and Drug Administration (FDA). Colors additives that are classified ‘exempt from certification’ or ‘certifiable’ or authorized for use in foods. Pigments that are derived from natural sources such as vegetables, minerals, or animals and manmade counter part
In gas chromatography, the components of the sample, (which can be called solutes or analytes), separate between two phases – a stationary phase that has a large surface area and a gas phase that permeates through the stationary surface (McNair & Miller, 1998). The technique begins when a few microliters of liquid sample are injected into a port and it becomes vaporized to a gas in the chamber. This starts the mobile phase (also known as the carrier gas). Then, an inert carrier gas is fed into the