Chromatography : A Laboratory Technique

1629 WordsJun 10, 20157 Pages
Introduction Chromatography, which was originally discovered and developed by Mikhail Semenovich Tswett in the early 1900s, is a laboratory technique used to separate and distinguish between components of a mixture. The various components of a sample mixture are known as analytes. Chromatography was originally used to separate the various molecules that make up plant pigmentation. Because these components, carotenes, chlorophyll, and xanthophylls, are different colors, the separation of them is responsible for the name of this laboratory technique. Throughout the early to mid-1900s, different styles of chromatography were developed and allowed this technique to be applicable to a wide variety of analyses and separation processes. Chromatography can be applied to analytical process, which aim to examine the presence and relative concentrations of various analytes within a sample. The goal of preparative chromatography is to separate analytes within a mixture for isolation and purification purposes. Chromatography was originally developed by Tswett for the purpose of isolation of particular compounds (10). Moreover, more recent advancements in chromatography are facilitating the separation and distinction of analytes that are extremely comparable to one another and increasing the resolution of the technique. Gas chromatography (GC) is a chromatography technique that was developed around the mid-1900s. In addition to being the first among chromatography equipment to be
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