Chromatography : A Laboratory Technique

Lab Report 4: Protein Purification: Size Exclusion Chromatography Badrun Nessa Rahman Lab Partner: Briana Tolbert Section 55 Objective: The purpose of today’s laboratory is to understand how size exclusion chromatography plays a role in purifying protein which is an important concept to understand as it helps answer the mysteries which lay behind proteins such as

material such as paper. This technique, called chromatography, is a forensic method of separating a mixture of chemicals into their individual components. Although the separation of colors is the most elementary application of chromatography, the process of a liquid or gas mixture moving over a stationary state of matter (liquid or solid) is a tool used by local and federal law enforcement to analyze and solve crimes. TYPES OF CHROMATOGRAPHY Capillary Action Chromatography In the past six decades

Goal: The first goal of today’s laboratory is to separate components of spinach dyes using different eluants. The four eluants will be using are ethanol, chloroform, 9:1 petroleum ether : ethanol, and petroleum ether. The second goal of the lab is to separate fluorine and fluorenone by column chromatography. Thin layer chromatography (TLC) was used to measure the polarity and separate the components in the mixtures. TLC was chosen because of its simplicity, high sensitivity, and speedy separation

COLUMN AND THIN LAYER CHROMATOGRAPHY Maria Janine B. Abarientos, Kuia B. Allarde, Aliana Keshia P. Andino Mary Viadelle E. Andrada and Nina Marian Robelea G. Ang Group 1 2C Pharmacy Organic Chemistry Laboratory ABSTRACT In this experiment, the techniques column and thin layer chromatography was used to separate and determine the purity of the colored components of siling labuyo and malunggay leaves. The results obtained relied on differential solubilities and adsorptivities of the components to

The detection and/or identification of trace amounts of explosives. The review of each method should include a description of the way the method works and the advantages and drawbacks of the technique. Introduction An explosive, are reactive substances that contain a large amount of potential energy that can yield an explosion if they are released rapidly, they are accompanied by the production of light, heat, sound, and pressure. Mainly used in the mining industry for extraction of minerals

investigating samples of polluted ground water will want to know which toxic ions might be present in a sample. Chromatography is one of the first tools used in such situations. In this technique, many types of mixtures can be separated into the component pure substances; by comparison to a standard sample, each component substance can also be tentatively identified. Many varieties of chromatography exist, each one designed to separate specific types of mixtures. The common feature of each type of

found of that the process of finding the colors was called Chromatography. Chromatography process uses chromatography paper, this paper absorbs the color and separates it. I am trying to found out more about chromatography and how it works as I'm doing my science fair project. I believe learning more about chromatography may help to explain why foods appear the color they do. Chromatography has been around for 100 of years. Chromatography was basically made in Russia in 1906 (Woodford,

are made in laboratories by people who are unskilled and untrained. Urine There are two steps for testing of drugs. One is screening and the other is confirmatory test. Screening test is used to find the presence of drug or to identify a specific drug. The screening test for drugs are cheap and less time consuming. But the drawback of this test is not precise as confirmatory test. For detection of drugs in urine, there is two screening methods such as immunoassay and chromatography. Urine samples

During this experiment the His-tagged protein RNase H was purified using affinity chromatography. The source of over-expressed His-tagged RNase H used was 5.0 mL of E. coli lysate. The experiment was performed in partners using different materials in order to prepare the needed three buffers to an approximate pH value of 7.9. Once the three buffers were ready a minicolumn was packed with 0.5 mL of Ni-NTA agarose and then different solutions were used to wash the column, starting with DI water, followed

Abstract Pigments extracted from different greens have different polarities and may be different colors. Mixed pigments can be separated using chromatography paper. Chromatography paper is able to separate mixed pigments due to their polarity and solubility. Pigments of chlorophyll a, chlorophyll b and beta carotene will be separated on chromatography paper because each has its own polarity and solubility, which results in different distance traveled up the paper. Beta carotene is non-polar so it