Detection Of The Four Major Biological Macromolecules

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Detection of the Four Major Biological Macromolecules Silvia Tian Gan 20631739 with Noor Helwa TA: Maple Ong, Vincent Halim Section Number: 023 BIOL 130L, Thursday, 9:30am~12:20pm, B2 151 Performed at Sep. 24th, 2015 Introduction In order for cells to carry out their daily activities, the aid from some biological molecules are needed. These molecules are categorized by biologists into four major groups, and are given the name macromolecules: carbohydrates, lipids, proteins, and nucleic acids. The identification of these macromolecules are crucial for biologists to gain a better understanding of cellular activities such as metabolism, respiration, and photosynthesis. The aim of this experiment is to identify the macromolecules by using different reagents, with the exception of nucleic acids and lipids. The experiment is divided into three sections, testing for proteins, complex carbohydrates (polysaccharides), and simple carbohydrates (monosaccharides and some disaccharides). A number of 12 samples are tested, including one unknown sample. Positive controls that were used for all the sections are protein solution, 1% starch solution, and 1% glucose solution, respectively. Distilled water is used as a negative control for all sections. The first section of the experiment is known as the biuret test, which detects the presence of proteins; or more

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