Enzyme Activity Lab Report

Enzyme catalysis is dependant upon factors such as concentration of enzyme and substrate, temperature and pH. These factors determine the rate of reaction, and an increase in temperature or pH above the optimum will

Hypothesis: I believe the rate of reaction will speed up as the temperature increases until it reaches about 37oC, which is the body temperature, where it will begin to slow down and stop reacting. I believe this will occur because enzymes have a temperature range at which they work best in and once the temperature goes out of this range the enzyme will stop working.

After added, pick up the beaker and swirl it around lightly for a short period of time.

3) Adding less enzyme caused the reaction to proceed more slowly than when more enzyme was utilized.

Discard the solution in the appropriate container as directed to you by your lab instructor.

8. Repeat step 7 with H2SO4, except that you should use a 10 mL graduated cylinder of H2SO4 and adding 15 mL water.

Measure 500ml of tap water in the 500cm3 beaker, then measure 5g of sodium hydrogen carbonate using the 50cm3 beaker and weight scale and place in the beaker of water, using the glass rod to dissolve it into the mixture.

The null hypothesis for the first experiment was that substrate concentration would have no effect on the reaction rate. It was hypothesized that the reaction rate would increase with rising substrate concentrations, until all active sites were bound. The null hypothesis for the second experiment was that temperature would not have an effect on reaction rates. It was hypothesized that until the enzyme is denatured, as temperature increased, so would the reaction rate.

If you see a deep blue color add more zinc powder until there is no change in color of the solution in the test tube.

1) Pour 25 mL of the 1 M hydrochloric acid into the beaker and rinse the solid by swirling the acid around in the bottom of the beaker.

1.) Transfer the distillate to separatory funnel. Fluid didn’t seem very clear but sufficient to finish our lab on time.

Grab the 50 ml graduated cylinder and fill it to the 50 mark line with the Diluted 3% hydrogen peroxide. Pour it into the other beaker.

Measure out 20mL of each concentration of hydrochloric acid into beakers, and label each beaker. Fill the 6th with 20mL of distilled water, as this will be your control. 2. Place a chalk piece in the beaker with water and time for 5 minutes. 3.

In this lab the effect of the enzyme concentration has on the speed of the reaction will be observed. The amount of oxygen gas produced will be measured to determine the reaction rate. If the enzyme concentration increases then the reaction rate will also increase. The measure of how fats oxygen is produced will be how long it takes for the filter paper disk soaked in different concentrations of catalase to rise to the top. If catalase is exposed to boiling temperature then it will denature.

3. Use a sterile pipette to transfer 0.1 ml of each dilution on to a MacConkey agar plate.