Escherichia Coli ( Coli ) For Antimicrobial Resistance Genes

1523 Words Dec 15th, 2015 7 Pages
Analysis Of Escherichia Coli (E. coli) For Antimicrobial Resistance Genes
Hetvi .B. Patel
Harper College

ABSTRACT This essay reviews a basic microbiological research project that focused on identifying pathogenic strains of Escherichia coli (E. coli) from the poultry industry in a developing country such as Romania. The research methodology, results and its practical applications are discussed. 51 isolates of E. coli were taken from various samples of raw poultry meat and tested for eleven specific antimicrobial resistance genes by using different techniques. Many antimicrobial resistant strains were isolated from the meat samples. These strains were confirmed by polymerase chain reaction (PCR)-based method to identify the pathogenic DNA. It was further revealed that these E. coli had genes which produced proteins against many antimicrobials, making them resistant. Fortunately, the most pathogenic, Shiga-toxic gene was not detected in any of the E. coli found in poultry meat. This research was very important for Romania because it would help lay a foundation for future studies and research regarding poultry food safety and precaution in Romania.

Introduction E. coli usually resides in the intestines of humans and is beneficial to humans and animals. Most of the E. coli strains are not harmful, although some of the E. coli variants are pathogenic in nature and cause various intestinal problems (CDC, 2015). Meat usually contains bacteria,…
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