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Importance Of The Reproductive Neurendocrine Prochans In Biology

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The evidence in the literature suggests that the reproductive neuroendocrine axis is centrally regulated through inputs from kisspeptin-expressing neurons to GNRH-expressing neurons, which afterward regulate the release of LH and FSH from the anterior pituitary [Aki et al., 2015]. Kisspeptin primarily participates in the regulator of the reproductive axis performed its capability to operate upon, and evoke GNRH neurons, which have been shown to express GPR54 [d’Anglemont and Colledge, 2010]. Vaccination with kisspeptin DNA vaccine results in the production of anti-kisspeptin antibodies that suppress GNRH and block the synthesis of testicular steroids and reduce kisspeptin concentration level in the blood. The result of the current study …show more content…

These findings suggested that P234 vaccination modulated the median eminence kisspeptin-kiss1r signaling take part in the kisspeptin-stimulated testosterone release and may not passed the blood-brain barrier. Furthermore, plasma kisspeptin, LH, and FSH levels in controls are significantly increased relative to animals with hypogonadotropic hypogonadism [Kotani et al., 2014]. Moreover, kisspeptin-54 concentration level in the blood of control group was significantly higher than treatment group [Messager et al., 2005]. Further, peptide 234 suppressed the synthesis of GNRH in both prepubertal and pubertal monkeys [Guerriero et al., 2012] and administration of peptide 234 delays the onset of puberty in rodents [Pineda et al., 2010]. Beale et al., [2014] reported that the maintenance of ARC-kisspeptin concentration is crucial for normal LH pulsatile release and estrous cycle and decrease the LH pulse frequency. Administration of kisspeptin antagonist reduced LH secretory response to intracervical vaccine immunization of kisspeptin. This cumulative evidence indicates that the kisspeptin antagonist inhibited GNRH neuron and would reduce hypothalamus GNRH secretion in vivo.

Hypothalamus, pituitary, and testicular tissues were collected to quantify mRNA levels using qPCR. Accordingly, kisspeptin, Kiss1r, and GNRH mRNA concentrations in the hypothalamus were significantly lower in the treatment group than in the control group (P <

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