4.Measure 35mL of warm water and add them into each of the 4 test tubes at about roughly the same time. It is essential that the water is warm. Do not seal the test tube.
The experiment is to observe a variety of chemical reactions and to identify patterns in
3. Under a fume hood, drops of concentrated HCl(aq) were added to each test tube.
Fill each test tube with 4 mL of water and 4 mL of 3.0% H_2 O_2. Make sure the pH of each solution is the same using the pH strips.
Purpose: The purpose of this experiment is to observe a variety of chemical reactions and to identify patterns in the conversion of reactants into products.
1 ml of water should be added to the first test tube and make a note. In the second test tube, 1 ml of methyl alcohol should be added. In the third test tube, 1 ml of hexane must be added. Lastly, the fourth test tube will be a control.
The first step that needed to be done in this experiment was adding hydrochloric acid (HCl)
Carefully fill a small test tube ½ full of bromothymol blue and mix it with the citric acid in the corner of the zip lock bag. Record your observations.
Step 3: Get the balloon ready for the first test tube and place a 1 cm strip of magnesium in the acid, quickly closing the balloon over the mouth of the test tube.
To begin the experiment, start by grabbing five cuvettes and labeling them one through five. After they have been labeled, add 2 mL of water to cuvette one, and 1mL of water to cuvette two through five. Then add 1mL of Catechol in cuvettes two through five. Because of the time considerations, test two cuvettes at a time. To do this, add 5 drops of Parahydroxybenzoic acid to cuvette two and 10 drops to cuvette three. Afterwards add 5 drops of Catechol Oxidase to cuvettes one through three. Blank out the spectrophotometer with cuvette one containing the water (H20). Then test the absorbency of cuvette two and three checking them every two minutes for twenty minutes, record your results. After those cuvettes have been tested, cuvette four and five need to be tested. Begin this by adding 15 drops of parahydroxybenzoic acid to cuvette four and 15 drops to cuvette five. Add in 5 drops of Catechol to cuvette four and five. Blank out the spectrophotometer again with cuvette one. Proceed to test cuvette four and five by checking the absorbance every 2 minutes for 20 minutes, record your
add one drop to the other test tube of that pair, which has the same
In this experiment, the concentration of an unknown permanganate solution will be determined. Spectroscopic techniques will be used to measure various known concentrations of permanganate solutions to create a calibration curve for the absorbance of light vs. the concentration and will be used to determine the unknown concentration1.
Collect to 2 large beakers both large beakers are to be filled with hot water (labtutor). Then obtain seven conical tubes these will be used to collect the levels of gas, you will also need test tube a stopper and a plastic tube (labtutor). You want to fill the conical tube to at least 50 ml of water (Cressy). Take the four conical tubes filled with water and place two in each beaker, to do this you must invert the tube and cover the release hole as to not lose any water (Cressy). Then place the beakers with the tubes in the bath so they can be at the same temperature as the bath (Cressy). Next mark all of your test tubes in number order to be sure which tube contains what concentrations and pH (Cressy). Having mixed a solution to the specifications of 2.5 ml of glucose in all tubes, 3 ml of yeast in 2 tubes of pH 5, 2 tubes of pH 9, and the single pH 7 tube, the remaining two tubes will contain no yeast as they will be negative controls. Next add 2 ml of pH buffer 3 tubes will receive pH of 5, three will receive a pH of 9 and a single tube of pH 7. Finally add pure water to make sure all test tubes have 10 ml of solution. When making the solutions
I then added 50 ml of distilled to the test tubes A1, B1, C1 and D1, then titrated them with the potassium permanganate solution (making sure to add 15 ml 3M sulfuric acid in each), then recorded the amount it took for them to turn pink.
4. Prepare pH solutions in each little bottle. The pH solutions used for the experiment are pH4, pH5, pH6, pH7, pH8, pH9. Use the pH meter to measure all the solution before staring each trial, to ensure each pH level is accurate. Record the data in logbook.