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Iodine Tests For Starch And Benedict 's Test For Sugar

Satisfactory Essays

Amylase is produced in two ways; one from person salivary glands called alpha-amylase and second from our pancreas, which is secreted in the small intestines known as pancreatic amylase. It is an enzyme that catalyses the starch to the disaccharide maltose. Salivary amylase is produced by the salivary glands and the pancreas produces pancreatic amylase. If amylase is added to a solution of starch, the starch will be consumed to form maltose (7). SUBSTRATE (starch) ENZYME (Amylase) → PRODUCTS (maltose + maltose + -- etc.--)
Iodine test for starch and Benedict 's test for sugar are used for this purpose (7). Iodine Test for Starch, Iodine solution is made of I2KI. It is easy to determine if a sample have starch on it. Iodine …show more content…

For example, having to test cooked brown rice and you measured a weight of 3.0 grams. You should get the same amount of cooked brown rice for the rest of experiment; you will attempt to do three trials for each sample.
4. If sample 1 is 3.0 grams, sample 2 and three should be 3.0 grams as well. As to be as consistent as possible.
5. Record weight of sample
6. Prepare three boiling test-tubed and add 10 cm3 of distilled water on each test-tube, put in a rack. Mark your sample accordingly. To distinguish the 3 samples being tested.
7. Select an appropriate time a person chew food. Record your time you decided, example 10 secs to a minute. Remember this will be used for all samples. Put the sample to a pestle and grind the food sample until the timer on the stopwatch has reached your desired amount of time. Grinding in the pestle and mortar represents chewing process.
8. Add few drops of distilled water in the sample during grinding. This will represent the saliva in the mouth during chewing. Predict which sample will have the most sugar.
9. Take visking tubing and transfer the sample content into it. Put the sample as much as possible in the bottom part of the tubing. Place the visking tubing in one of the boiling test tube. Repeat procedure 3 to 9 for next two trials.
10. Do the same procedure for the other sample to be tested.
11. Add a 2 drops of amylase solution on each test-tube. And put all test samples in warm bath equipment for about 30 minutes. At

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