Amylase is produced in two ways; one from person salivary glands called alpha-amylase and second from our pancreas, which is secreted in the small intestines known as pancreatic amylase. It is an enzyme that catalyses the starch to the disaccharide maltose. Salivary amylase is produced by the salivary glands and the pancreas produces pancreatic amylase. If amylase is added to a solution of starch, the starch will be consumed to form maltose (7). SUBSTRATE (starch) ENZYME (Amylase) → PRODUCTS (maltose + maltose + -- etc.--)
Iodine test for starch and Benedict 's test for sugar are used for this purpose (7). Iodine Test for Starch, Iodine solution is made of I2KI. It is easy to determine if a sample have starch on it. Iodine
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For example, having to test cooked brown rice and you measured a weight of 3.0 grams. You should get the same amount of cooked brown rice for the rest of experiment; you will attempt to do three trials for each sample.
4. If sample 1 is 3.0 grams, sample 2 and three should be 3.0 grams as well. As to be as consistent as possible.
5. Record weight of sample
6. Prepare three boiling test-tubed and add 10 cm3 of distilled water on each test-tube, put in a rack. Mark your sample accordingly. To distinguish the 3 samples being tested.
7. Select an appropriate time a person chew food. Record your time you decided, example 10 secs to a minute. Remember this will be used for all samples. Put the sample to a pestle and grind the food sample until the timer on the stopwatch has reached your desired amount of time. Grinding in the pestle and mortar represents chewing process.
8. Add few drops of distilled water in the sample during grinding. This will represent the saliva in the mouth during chewing. Predict which sample will have the most sugar.
9. Take visking tubing and transfer the sample content into it. Put the sample as much as possible in the bottom part of the tubing. Place the visking tubing in one of the boiling test tube. Repeat procedure 3 to 9 for next two trials.
10. Do the same procedure for the other sample to be tested.
11. Add a 2 drops of amylase solution on each test-tube. And put all test samples in warm bath equipment for about 30 minutes. At
Gum chewing has been around for at least 3,000 years, all around the world. Gum chewing was around during the ancient greeks, the mayans, and was introduced to americans in the late 1860’s. The first bubble gum was sold in 1928 and was pink because that was the only color in the factory; pink is still one of the most popular colors of chewing gum today. In this experiment, I will be finding the percentage of sugar in the Bubbilisious Strawberry gum. To find this I will need to record the data of 625 chews, recording the mass every 25 chews.
Pick up each test tube, tap the bottom/ swirl the contents and then using your ruler record in Table 1 the bubble height (if any) that is produced. Remember to include this table (with a strong title) in your lab report.
Stopclock This is important for measuring the time. It is not important whether a digital or traditional stopclock is used as there will be the same percentage error either way. Method * Measure out 1 cm³ amylase solution with calibrated syringe and put in corvette *
Procedure: First you will need to get all the lab equipment and material that is needed to do this experiment. After that fill the 150ml beaker to ¼ full of water. Than you will be provided by a teacher or an instructor who will give copper with an scoopula. Than pour the copper that was provided into the beaker, before you stir
Fill a test tube about 1/3 full with cold tap water for use in step 34.
1.) Measure out 20ml out of the water and place it into a glass beaker
Stop the trial and restart, selecting the option to record data at 10-minute intervals. Slowly insert nutrients and allow the water pump to disperse them and dissolve them. Once the nutrient is completely added in and dissolved, turn off the pump by unplugging it. Also record the time in the trial that the pump was turned
4.Measure 35mL of warm water and add them into each of the 4 test tubes at about roughly the same time. It is essential that the water is warm. Do not seal the test tube.
1 ml of water should be added to the first test tube and make a note. In the second test tube, 1 ml of methyl alcohol should be added. In the third test tube, 1 ml of hexane must be added. Lastly, the fourth test tube will be a control.
1. A representative sample was chosen by quartering (according to BS 812: Part 102: 1984) or by use of a sample splitter (Fig. 2C1-3). The sample to be tested should be the approximate weight desired when dry. For this experiment, about 3 kilograms of coarse aggregate was weighed.
Empty and clean the test tubes and repeat steps 2-10 again for a second dataset.
1. Number four clean test tubes 1-4. 2. Place 1 ml (or 20 drops) of the following solutions into each tube: water, egg albumin, starch, and chicken broth. 3. Add 3 drops of Buiret reagent to each tube and gently shake
Collect to 2 large beakers both large beakers are to be filled with hot water (labtutor). Then obtain seven conical tubes these will be used to collect the levels of gas, you will also need test tube a stopper and a plastic tube (labtutor). You want to fill the conical tube to at least 50 ml of water (Cressy). Take the four conical tubes filled with water and place two in each beaker, to do this you must invert the tube and cover the release hole as to not lose any water (Cressy). Then place the beakers with the tubes in the bath so they can be at the same temperature as the bath (Cressy). Next mark all of your test tubes in number order to be sure which tube contains what concentrations and pH (Cressy). Having mixed a solution to the specifications of 2.5 ml of glucose in all tubes, 3 ml of yeast in 2 tubes of pH 5, 2 tubes of pH 9, and the single pH 7 tube, the remaining two tubes will contain no yeast as they will be negative controls. Next add 2 ml of pH buffer 3 tubes will receive pH of 5, three will receive a pH of 9 and a single tube of pH 7. Finally add pure water to make sure all test tubes have 10 ml of solution. When making the solutions
(6.2)Material and Methods in the process or exercise of measuring the starch we were used the following material and how we used them to conduct the experiment. Obtain seven tubes the material to be tested table 6.1 and then add seven to ten drops of iodine to each tube, and then record the color of the tubes contents in table 6.1
8.After 5 minutes, remove test tubes from the water bath set in test tube holder and record results. Also turn off hot plate.