Kreb Biology Lab

Satisfactory Essays
In this lab, I investigated aerobic respiration in the Krebs cycle through the means of a color indicator. The Krebs cycle is a vital metabolic pathway that occurs in the mitochondria and produces energy utilized by living cells. In order for the Krebs cycle to occur, glucose needs to combine with oxygen. Furthermore, in this lab, I used Malonate as an inhibitor; a competitive inhibitor binds to an enzyme’s active site inhibiting the substrate from binding which ceases the production.
The various tubes we tested in the spectrophotometer contained different combinations of bean extract, DPIP, phosphate buffer, malonate, and succinate. Tube 1 did not contain any Malonate, and the absorbance values mainly decreased. In
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