Osmosis in Potato Cells
I am going to do an experiment to find the concentration of solute in potato cells. In order to find the best way to do this experiment I am going to do a preliminary test first.
For my preliminary test I decided to use potato cylinders 3cm long and I would see if the cylinders increased or decreased in mass. I used salt solution in three different concentrates: 0.0M, 0.5M and 1.0M. I put 20cm3 of each concentrate into 3 different boiling tubes, which were then placed in test tube racks. I then placed a cylinder of potato in each boiling tube and left them for three days. My results were inconclusive because I left them in the solutions for too long so the potato
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I will then use the same potato to carefully cut 11 3cm long potato cylinders using an apple corer. I will then cut each cylinder into 10 separate small slices using a scalpel and carefully weigh them and take the mass to 2 decimal places. I will then record the masses and place them into the solution, making sure I know which mass went into which solution. I will leave them for 2 days and then weigh them again. I will then repeat the experiment again so I can take an average of my results so they are more accurate. I am doing the test this way because I feel that this is the most accurate way I can perform this experiment realistically in a school lab.
I will make sure it is a fair test by making sure that I put exactly 20cm3 of solution in each boiling tube, and that I mix the concentrates accurately and wash out the measuring cylinders after each measurement, making sure there are no drops of water or salt solution left in the bottom. I must make sure that the potato is healthy before I start using it, and make sure that my cylinders are cut neatly and are measured accurately. I must take care to make sure I do not loose any of the small slices of potato when measure before and after the experiment, I must also take when using the balance to make sure it reads 0.0 before I
Aim: To find the concentration of a potato cell cytoplasm compared to sucrose and salt. I mainly was looking to see how concentration of salt/sucrose affects the mass of the potato cores.
In this experiment first the stages of an onion cell undergoing mitosis are going to be observed and every stage is going to be detected and drawn on paper. A brief description to what is going on should be attached to the pictures. This is important to understand the basics of cell division which is necessary growth,repair and asexual reproduction. Second the number of cells undergoing each phase is going to be counted to figure out in which phase the cell remains the most. If interphase is the stage in which the cell grows and prepares for cell division then the
You may have observed the reaction of naturally-occurring catalase in tissue from either liver of potato. Design an experiment to determine if the amount of catalase varies from tissue to tissue (e.g. 200 g of liver compared to 200 g of potato).
In this lab experiment, half our group observed and measured osmosis using dialysis tubes that were represented as the semipermeable membrane. It is permeable to water and other small molecules but is impermeable to larger molecules such as the sucrose solution used in each of the four beakers and tubing. The other half of our group observed the tonicity of sheep blood to determine whether the blood was isotonic, hypotonic, or hypertonic. The 85 g/dL of NaCl solution was the ideal isotonic number in relation to the sheep blood cells as well as a reference to the other observations of the solutions.
Purpose: To determine the concentration of solute in the potato’s cytoplasm by measuring the change
Purpose: The purpose of this lab is to familiarize you with osmosis and, specifically, what happens to cells when they are exposed to solutions of differing tonicities.
After complete, continue using HCl and NaOH, but now testing potato cell, liver cell, and the buffer (one at a time)
Repeated Trials: In procedure one, we tested diffusion on different sized cell models (gelatin with various volume and surface area wise). In procedure two, we tested cell models in different internal environments and similar external environments to find the effects on rate of osmosis. In procedure three, we used potato cells in different concentrated sucrose environments to test the effects on water potential on cells and osmosis.
If Michael’s mistake had been caught earlier, is there anything that could have been done to prevent the corn from dying?
Measure and add 5cm3 of buffer solution using a measuring cylinder with the pH 3 into a test tube using a pipette and place the potato cylinders into the test tube.
The following hypothesis was made in regard to effect of the concentration gradient on the rate of diffusion: The higher the concentration gradient, the faster the rate of diffusion.
Van’t Hoff’s Law suggests that the osmotic potential of a cell is proportional to the concentration of solute particles in a solution. The purpose of this experiment was to determine if there are any differences between the osmolalities, the no-weight-changes of osmolalities, and the water potentials of potato cores in different solutions of different solutes. The percent weight change of the potato cores was calculated through a “change in weight” method. The potato core’s weight was measured before and after they were put into different concentrations of a solute for 1.5 hours. In our experiment, there were no significant differences from the osmotic potentials of our results and the osmotic potentials of other scientists work. Ending with chi square values of 2.17 and 2.71, and p values of 0.256 and 0.337, concluding that there is no difference in water potentials of potato cores in different solutions of different solutes at varying concentrations.
The aim of the sixteenth of November experiments was to observe how three different solutions with various sucrose concentration influenced osmosis in relation to three onion cells and the impact on the cells structure.
Cells in all living things have an outer layer known as the cell membrane. The structure of the cell membrane consists of the phospholipid bilayer organized by the arrangement of hydrophilic heads and hydrophobic tails. It is a selectively permeable membrane, where it divides the outer environment from the interior of the cell. It can control substances moving in and out of living cells. Certain molecules like gases, water, and food are permitted to pass the membrane through the method of diffusion. Diffusion refers to the process in which molecules move on the concentration gradient, where they move from an area of high concentration to an area of low concentration. A type of diffusion is known as osmosis. It is the diffusion of water moving across the selectively permeable membrane. In this lab, students will be using eggs to construct an experiment to get a better study on how osmosis works in a cell. The eggs will be soaked in vinegar solution to remove their shells to expose each inner layer that resembles a selectively permeable membrane. The egg shell is composed of calcium carbonate that would dissolves in acidic solution such as vinegar. In the chemical reaction, it releases carbon dioxide gas. After the removal of the egg shell, it will be ready to be able to construct the experiment.
However one beaker received 100 mL of Deionized water with a molarity of 0.0. Afterwards a cork borer was pushed through the potato and was twisted back and forth. Once the borer was filled it was removed from the potato. Pushing the potato cylinder out of the borer, this this step was repeated six more times in order to get seven undamaged potato cylinders. Using a sharp razor blade, the potato cylinders were both cut to a uniform length of about 5cm, and were removed of their potato skins. The potato pieces were also cut in half to give the cells a greater surface area in which it was easier to absorb the solution. After the cylinders were weighed on a balance and the data was recorded in Table 4. Using the razor blade each potato was cut lengthwise into two long halves. Then the potato pieces were transferred to the water beaker and the time they were submerged was recorded. This step was repeated for all potato cylinders in which the pieces were placed in solutions 0.1 to 0.6 M. The potatoes were incubated for ninety minutes. At the end of the incubation period the time was recorded. Then the potato piece was removed form the first sample. Next potato pieces were weighed the and the final weight was recorded in Table 4. This procedure was repeated until all samples had been weighed and recorded in the chronological order they were initially placed in the test solution. Afterwards the table was completed by recording the