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Sweat Cell Development : Nude Mice

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Results Time course of Matrigel-embedded eccrine sweat cell development in nude mice On the first day of implantation, cells in the Matrigel plugs were scattered primarily as single cells, with 2- to 4-cell clusters occasionally observed (Fig. 1A, arrowhead). On the second day, the cells formed small solid spheroid-like cell masses (Fig. 1B). From the fourth to eighth day, the size of the solid spheroid-like cell masses gradually increased (Fig. 1C-E). From day 14 on, hollow lumens began to appear in the spheroid-like structures (Fig. 1F, arrow). With time increased, the number of spheroid-like structures with one or more lumens lined by one or more layers of cells also increased (Fig. 1G-J). The formed secretory coil-like structures and coiled duct-like structures [12] were similar to the secretory coils and coiled ducts of native human eccrine sweat glands, respectively (Fig. 1K). Matrigel-embedded eccrine sweat cells rapidly enter the cell cycle, but cell proliferation subsequently decreases with time Immunostaining revealed that Ki67 was restricted to the nuclei (Fig. 2A1-3~J1-3) and the negative control revealed background staining (Fig. 2K1-3). Ki67-positive cells were present at all time points (Fig. 2A1-3~J1-3). The Ki67 labeling index on the first day of implantation was 30.532.47%, and reached a peak value of 81.43±5.44% on the second day (Fig. 2K). Subsequently, the Ki67 labeling index gradually decreased with time, reaching a low of 2.87±1.27% at 42 days after

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