Taking a Look at Neurotransmitter Release

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Neurotransmitter release is a key step to neuro-transmission, in the middle of the 20th century; it has been clearly revealed that chemical signals transfer the great majority of the information in the brain. Therefore, different neurochemistry approaches have been emerged to investigate neurotransmitter release process. Later, introducing a radiolabeled precursor into the CNS and measuring the accumulation of a radiolabeled product have made critical improving and developing in vitro preparations approaches including isolated nerve ending fractions or synaptosomes, cultured cells and tissue slices. However, in this study we focused on the use of brain slices, as well as intact structure giving a close picture of th in vivos situation. The tissue slice method was introduced by Warburg in 1920s to investigate the energy metabolism of tissues slice and Henry McIlwain extended it to study brain metabolism. Currently, slices structure is an accepted technique for studying many aspects of neurobiology. Slices are made using a mechanical device, such as a tissue chopper or vibrato me. Subsequently, they are immediately transferred into cold carbongenated iso-osmotic salt solution. Tissue slices are used with and without radiolabeled tracers dependent on the case of studies. Subsequently they are transferred into small chamber and superfused 1 hour with fresh carbongenated medium to achieve stability. Following equilibration slices can then be exposed to chemical and/or electrical
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