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The Effect of Glucose Concentration on Anaerobic Respiration in Yeast

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THE EFFECT OF GLUCOSE CONCENTRATION ON ANAEROBIC RESPIRATION IN YEAST

INTRODUCTION:

Yeast, also known as a saccharomyces cerevisiae, is single celled eukaryotic cells that are in the kingdom fungi and are unicellular organisms which normally reproduce asexually by budding at a very high rate. Scientists quite often decide to work with yeast because of its features fast growing rate and the fact that yeast 's DNA can be easily manipulated. Some types of yeast can be found naturally on plant or in the soil. Also it is worthwhile mentioning that yeast feeds on sugar very well, and so that I decided to use glucose.

My independent variable will be the concentration of glucose solution. To make sure that that my test will be fair, I will make
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Temperature

Measured by a thermometer

C: Varies throughout the day, but each yeast solutions gets the same temperature

It is important to keep the temperature the same throughout the whole experiment because enzymes might work faster or slower depending on the temperature.

DEPENDENT VARIABLE:

Burette

Placing portions of different concentration of yeast solution separately in the apparatus

Count the amount of bubbles every 1 minute

To read the burette correctly it is important to remember that the lowest numbers start on top and go up to the highest numbers at the bottom.

The curved surface at the top of the liquid level is called a MENISCUS.

METHOD:

Weigh four different amounts of glucose 1g,2g,3g,4g and 5g and place each amount into a conical flask .

Add 100 ml of water to the conical flask and swirl until the glucose is fully dissolved.

Yeast has to be heated up before mixing it with
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