The conceptual overview, along with the experimental procedures are found in the NPB 101L Physiology Lab Manual 2nd Edition. More specifically, pages 55 to 63 provided a full insight to all the experiments in the respiratory lab. In addition, a list of required materials was given for every experiment. The authors of this lab manual were Erwin Bautista and Julia Korber. As for data acquisition, the BIOPAC software was used to collect and record data for all the trials. Furthermore, the teaching assistants had calibrated each station prior to initiating the lab exercises. For the entirety of the respiratory lab, a human subject was used in all the experiments. A 22-year-old female was the participant for the first two experiments regarding …show more content…
The same method was utilized to find expiratory reserve volume. Except, the I-beam cursor was dragged in-between the trough of a normal and maximum exhalation. As for tidal volume, it was discovered using the absolute value between the peak and the trough of a normal wave. The last data to be found using the BIOPAC was the subject’s vital capacity. Here, the I-beam cursor was dragged from the peak of the maximum inhalation to the trough of maximum exhalation. Other vital information that can be extracted from the BIOPAC software was the respiratory rate of the subject. Counting every wave peak for the last 30 seconds and multiplying the value by 2 yielded the respiratory rate. Having these information, minute ventilation can now be calculated by multiplying respiratory rate with tidal wave volume. This led to the calculation of alveolar ventilation since the formula is minute ventilation minus dead space volume. In order to find out the subject’s dead space volume, her weight was multiplied with the respiratory rate. Moving on to Part 2 of the respiratory lab, the experiment was designed to observe the difference between length of breath-hold and ventilation type. These ventilation types consist of normal breathing, re-breathing, and hyperventilating. The second component to part 2 was to see a correlation between the
After clicking Reset and running the experiment again, the function of the simulated lungs returned to normal. This happened because the air was removed from the intrapleural space, allowing the lungs to reinflate. Intrapleural pressure was decreased below atmospheric pressure by clicking Reset.
The vials were placed in the water and the oxygen levels were read every 5 minutes until 30 minutes was reached. As the oxygen levels were collected they were put into a table that had all three tubes labeled at each 5-minute increment. After 30 minutes the experiment was completed and the clean up process could begin.
A normal respiratory rate is between 12 & 20 breaths per minute, this can be recorded manually by using a clock. If you respiratory rate drops below the normal measurements
1. Dependent Variable. respiratory volumes 2. Independent Variable. level of physical activity [resting or exercising] 3. Controlled Variables. height; age; sex 4. Which respiratory volume was calculated? Breating rate, TV, ERV, and IRV. 5. What was the purpose of the nose clip? the nose clip was used for the lung function testing to prevent leakage with
The higher her respiratory rate the more liters of oxygen needed to supply for the loss in her lungs. To get the minute ventilation you take the breaths per min times 500mL/breath and that gets your liters of oxygen per min.
Use of daily spontaneous breathing trials to assess the patient’s ability to sustain ventilation, oxygenation, and breathing.
Emmanuel and his mother live in an urban community-housing complex. The building is worn down and dirty from the urban dust, cockroaches, and mold. Emmanuel is five years of age and has suffered from asthma for the last two years. One evening, his mother poured him some milk and put him to bed. Shortly afterward, Emmanuel woke up wheezing and coughing. As he gasped for air, he became more and more anxious. His mother ran for his inhaler, but he was too upset and restless to use it. Emmanuel’s skin became moist with sweat, and as he began to tire, his wheezing became quieter. His mother called 911 and waited anxiously for the ambulance to arrive.
1) Make a graph of resting heart rates from Activity 1. Provide a physiological explanation for fluctuations in resting heart rate over time. Discuss a couple of extrinsic factors that influence the autonomic nervous regulation of resting heart rate.
Once the patient was correctly hooked up to the EKG the BIOPAC Student Lab Program was started. Lesson five is the one we used for this experiment and once it had been chosen we label it and started the experiment. There were four conditions we needed to measure; the first being lying down. The subject was lying down relaxing on the cot. We clicked record and let it run for 20 seconds. The data resembled the chart below. If it did not we would have had to repeat the steps until it did.
Patients had to measure their IC by using an incentive spirometer to measure static lung values. They performed this test for 20 minutes after inhaling 400 mg of salbutamol via a nebulizer. The patients were asked to use the FVC spirometer and told to take a deep breath and then to let the breath out passively. They were then asked to do the same maneuver 2 more times, but the closeness made the study choose the first attempt.
The purpose of the Cellular Respiration lab was to identify if cellular respiration is sped up due to exercise. To conduct the experiment each student filled a beaker with 15 ml of water and three drops of bromothymol blue. Next each student blew into the beaker through a straw until the water mix turns yellow. When carbon dioxide is added too bromothymol blue it changes to a yellow color. Each student times how long it takes for the water to turn yellow, records the data, and then reset the lab. Subsequently the student would exercise for a total of five minutes and then repeating the lab, again recording how long it takes for the water mix to turn yellow.
Exercise 7: Respiratory System Mechanics: Activity 2: Comparative Spirometry Lab Report Pre-lab Quiz Results You scored 100% by answering 5 out of 5 questions correctly. 1. A normal resting tidal volume is expected to be around You correctly answered: d. 500 ml. 2. Which respiratory process is impaired the most by emphysema? You correctly answered: c. expiration 3. During an asthma attack You correctly answered: b. inspiration and expiration are impaired. 4. During moderate aerobic exercise, which respiratory variable increases the most? You correctly answered: a. tidal volume 5. Inhaler medications for an asthma patient are designed to You correctly answered: b. dilate the patient's bronchioles.
At the end of the fifth minute the amount of oxygen in the test tube increased to compare with the color of tube number 4 on the chart. In part two, the amount of oxygen in tubes one and nine were equivalent at each minute. Tubes one, two, four, nine, and eleven all have initial oxygen levels comparable to tube number one on the chart. Tube twelve initially had an oxygen level comparable to tube number zero. For the next five minutes tube twelve maintained the same oxygen level as tube number one. At minute one, tubes one, four, nine, and eleven had oxygen levels similar to tube number six on the chart. Tube two had levels similar to tube seven. At minute two, tubes one, two, and nine had oxygen levels like tube number eight on the chart. Tubes four and eleven both had levels similar to tube seven on the chart. At minute three, tubes one, two, nine, and eleven all had oxygen levels similar to tube eight. Tube four was the only tube to have the same level of oxygen as tube seven. At minutes four and five, all but tube 12 had oxygen levels equivalent to tube
Spirometry is the most popular lung function test. The patient performs a maximal inhalation and then forcefully exhales as quickly and as long as they are able. The spirometer measures the volume of the air exhaled by patients. These measurements are taken at two intervals. The first measurement is the forced expiratory volume in one second (FEV1), records the volume of air exhaled after one second. The second measurement is taken at the point where the patient has fully exhaled the volume of inhaled air; this measurement is the forced vital capacity (FVC) (Harpreet Ranu et al.,
Carry out an experiment to measure the heart rate and ventilation rate before, during and after moderate exercise.