. Millions Test Color of Millon's reagent: Sample glutamic acid tyrosine casein albumin gelatin Color of original solution Color after Millon's Reagent Color after 5 min.
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Experiment
AMINO ACIDS AND PROTEINS
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- When acids are added to a solution, the pH should ___________ . a. decrease b. increase c. stay the same d. cannot tell without testing12. Identify what test is being described: Test for the presence of indole group ? A. HydrolysisB. DenaturationC. Heat denaturationD. Organic solvent denaturationE. Biuret testF. Hopkins – Cole ReactionG. Millon’s testH. Ninhydrin TestI. Sulfur testJ. Xanthroproteic TestK. ChromatographyL. Paper chromatographyM. Competitive inhibitionN. Noncompetitive inhibitionO. RancidityP. Hydrogenation 13. Identify what test is being described: Process of adding hydrogen to unsaturated fatty acids ? A. HydrolysisB. DenaturationC. Heat denaturationD. Organic solvent denaturationE. Biuret testF. Hopkins – Cole ReactionG. Millon’s testH. Ninhydrin TestI. Sulfur testJ. Xanthroproteic TestK. ChromatographyL. Paper chromatographyM. Competitive inhibitionN. Noncompetitive inhibitionO. RancidityP. Hydrogenation 14. Identify what test is being described: Yields blue color solution for proteins containing fewer than two peptide bonds. ? A. HydrolysisB. DenaturationC. Heat denaturationD. Organic solvent…11. ● Possible methods for the quantitative determination of sodium bicarbonate for injection are: A) Acidimetry B) Alkalimetry C) Complexometry D) Iodometry E) Refractometry ● Calcium chloride is available in dosage forms: A) Powder for preparation of injections B) Solution for injection 10% in ampoules C) Tablets of 0.3 g D) Solution for injection 25% in ampoules E) Dry dosed powder
- Explain the chemical basis (reaction) of Millon’s test. (Generic reaction and explanation) What is the difference between Millon's test and Biuret test?(minimum 5)Based on this video https://www.youtube.com/watch?v=rKng5-ij6kQ Provide a schematic diagram for the Iodine test methodologies in determining the presence of carbohydrates. Also, give the basic principle for the test.Solution Absorbance mg/ml aspirin Standard solution - 1.6 mg/mL A 0.638 0.08 mg/mL B 0.504 0.064 mg/mL C 0.376 0.048 mg/mL D 0.259 0.032 mg/mL E 0.126 0.016 mg/mL A = -log T where T = %T ÷ 100 Construct a callibration curve using the above data. Absorbance should be on the vertical axis and "mg/mL of acetylsalicylic acid" on the horizontal axis. The line should go through the origin. Using the data provided, the graph you have generated, and the procedure that was used to generate the solutions which were examined by spectroscopy, calculate the amount of acetylsalicylic acid per tablet. Commercial tablet 1 labelled as 100 mg enteric coated Absorbance = 0.16 Commercial tablet 2 labelled as 300 mg Absorbance = 0.45 Student prepared tablet from practical 5 Absorbance = 0.19 Using the data provided, the graph you have generated, and the procedure that was used…
- 1. The prescription for an oral solution reads “1 tbsp ac & hs x 10d.” What is the minimum (in mL) of solution should you dispense? 2. A nursing floor requires half liter of 50% isopropyl alcohol. How many mL of 70% isopropyl alcohol will be needed for compounding this order? 3. How may grams of pure dextrose is needed to prepare 100cc of D50W?REAGENTS. Match the name of the reagent with the physiological test. Tests may require more than one answer. a. alpha-naphthol b. dimethyl-alpha-naphthylamine c. ferric chloride d. Gram’s iodine e. hydrogen peroxide f. Kovac’s reagent g. methyl red h. N,N,N′,N′-tetramethyl-p-phenylenediamine dihydrochloride i. potassium hydroxide (KOH) j. sulfanilic acid 2,3-butanediol fermentation catalase test mixed-acid fermentation nitrate reduction oxidase test phenylalanine deamination starch hydrolysis tryptophan degradationQualitative and Quantitative Tests for Amino Acids and ProteinsComplete the table: Test Reagents Positive Result Positive Indication Ninhydrin test Xanthoproteic acid test Millon's test Hopkins Cole test Pauly's diazo Test Lead sulphide test Histidine test Isatin test Folin’s McCarthy Sullivan Test Nitroprusside Test
- 1. Prepare 200 mL of an isotonic aqueous solution of thimerosal (MW 404.84; L iso 3.4). The concentration of this antiinfective drug is 1:5000. a. Calculate the E value. b. Adjust the Isotonicity of the solution using NaCl.1&3/4 tbs po BID x 7 days #QS How many milliliters should the pharmacy dispense? this isnt 364 mL____________is the iodine antiseptic of choice for wound treatment.a. 8% tincture b. 5% aqueous c. 5% Iodophor d. 7.5% potassium iodide solution
