Homework Help is Here – Start Your Trial Now!
Science
Biology
1) Analyse the following experimental set-up (illustrated in Figure 1) a) A wild-type (WT) bacterial strain is sensitive to the antibiotic X (Ant X) (Figure 1A) b) You have grown the WT strains in 3 independent liquid cultures (C1, C2 and C3) for 24 hours (Figure 1B-Passage 1) c) Following this initial growth, C1, C2 and C3 cultures are subcultured in 9 independent cultures and grown for 24 hours (Figure 1C Passage 2). Subsequently, cultures are plated on Non-Selective (N/S) solid media and media with Ant X (+ Ant X). The number of colonies grown in each plate are shown (Figure 1C) Use the above information to discuss: a. the type of mutations (if any) that have been arisen during the experiment. b. When did the mutations arise

1) Analyse the following experimental set-up (illustrated in Figure 1) a) A wild-type (WT) bacterial strain is sensitive to the antibiotic X (Ant X) (Figure 1A) b) You have grown the WT strains in 3 independent liquid cultures (C1, C2 and C3) for 24 hours (Figure 1B-Passage 1) c) Following this initial growth, C1, C2 and C3 cultures are subcultured in 9 independent cultures and grown for 24 hours (Figure 1C Passage 2). Subsequently, cultures are plated on Non-Selective (N/S) solid media and media with Ant X (+ Ant X). The number of colonies grown in each plate are shown (Figure 1C) Use the above information to discuss: a. the type of mutations (if any) that have been arisen during the experiment. b. When did the mutations arise

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
See similar textbooks
icon
Related questions
icon
Concept explainers
Question
1) Analyse the following experimental set-up (illustrated in Figure 1) a) A wild-type (WT) bacterial strain is sensitive to the antibiotic X (Ant X) (Figure 1A) b) You have grown the WT strains in 3 independent liquid cultures (C1, C2 and C3) for 24 hours (Figure 1B-Passage 1) c) Following this initial growth, C1, C2 and C3 cultures are subcultured in 9 independent cultures and grown for 24 hours (Figure 1C Passage 2). Subsequently, cultures are plated on Non-Selective (N/S) solid media and media with Ant X (+ Ant X). The number of colonies grown in each plate are shown (Figure 1C) Use the above information to discuss: a. the type of mutations (if any) that have been arisen during the experiment. b. When did the mutations arise
Transcribed Image Text:1) Analyse the following experimental set-up (illustrated in Figure 1) a) A wild-type (WT) bacterial strain is sensitive to the antibiotic X (Ant X) (Figure 1A) b) You have grown the WT strains in 3 independent liquid cultures (C1, C2 and C3) for 24 hours (Figure 1B-Passage 1) c) Following this initial growth, C1, C2 and C3 cultures are subcultured in 9 independent cultures and grown for 24 hours (Figure 1C Passage 2). Subsequently, cultures are plated on Non-Selective (N/S) solid media and media with Ant X (+ Ant X). The number of colonies grown in each plate are shown (Figure 1C) Use the above information to discuss: a. the type of mutations (if any) that have been arisen during the experiment. b. When did the mutations arise
Figure 1 A C Passage 2 24 Hours N/S + Ant X 24 Hours N/S + Ant X 24 Hours N/S + Ant X 1.1 2.1 N 3.1 N/S + Ant X 1.2 2.2 3.2 WT 1.3 2.3 3.3 1.4 2.4 3.4 C1 B 1.5 C2 2.5 2 C3 3.5 Passage 1 1.6 2.6 3.6 WT 1.7 2.7 3.7 1.8 2.8 3.8 24 Hours 1.9 2.9 3.9 C3
Transcribed Image Text:Figure 1 A C Passage 2 24 Hours N/S + Ant X 24 Hours N/S + Ant X 24 Hours N/S + Ant X 1.1 2.1 N 3.1 N/S + Ant X 1.2 2.2 3.2 WT 1.3 2.3 3.3 1.4 2.4 3.4 C1 B 1.5 C2 2.5 2 C3 3.5 Passage 1 1.6 2.6 3.6 WT 1.7 2.7 3.7 1.8 2.8 3.8 24 Hours 1.9 2.9 3.9 C3
Expert Solution
steps

Step by step

Solved in 3 steps

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Knowledge Booster
Microscopic examination
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education
SEE MORE TEXTBOOKS