1 ml of whole blood is collected and centrifuged to obtain a plasma sample. The volume of the plasma sample is 300 microlitres. A 1 in 4 dilution of the plasma is made and analysed using a cholesterol assay like the one you performed in the laboratory. 10 microlitres of both the diluted plasma sample and a cholesterol standard are assayed using 1mL of cholesterol reagent. Following incubation at 37 degrees Celcius, the samples are measured spectrophotometrically at 500nm. If the absorbance of the plasma sample assay was 0.5, the absorbance of the cholesterol standard assay was 0.4, and the concentration of cholesterol in the standard was 1.3mM, what was the concentration of the cholesterol in the whole blood (in mM)? (assume all of the cholesterol in the whole blood sample was recovered in the plasma; do not include units in your answer; give your answer to 2 decimal places)

1 ml of whole blood is collected and centrifuged to obtain a plasma sample. The volume of the plasma sample is 300 microlitres. A 1 in 4 dilution of the plasma is made and analysed using a cholesterol assay like the one you performed in the laboratory. 10 microlitres of both the diluted plasma sample and a cholesterol standard are assayed using 1mL of cholesterol reagent. Following incubation at 37 degrees Celcius, the samples are measured spectrophotometrically at 500nm. If the absorbance of the plasma sample assay was 0.5, the absorbance of the cholesterol standard assay was 0.4, and the concentration of cholesterol in the standard was 1.3mM, what was the concentration of the cholesterol in the whole blood (in mM)? (assume all of the cholesterol in the whole blood sample was recovered in the plasma; do not include units in your answer; give your answer to 2 decimal places)

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter2: Basic Hematology

Section2.13: Erythrocyte Sedimentation Rate

Problem 1CT

See similar textbooks

Similar questions

Which sample has the greater chance to be hemolyzed, evacuated tube drawn sample or a syringe drawn sample? Explain why. Why is it important to gently mix evacuated blood tubes with anticoagulants or additives? When collecting blood in an evacuated tube with an anticoagulant, what is the importance of having the correct volume of blood? What is the principle behind the use of the evacuated tube? List 5 most common errors encountered during blood extraction using ETS.
Please explain: A laboratory scientist working the night shift receives a sample for send-out testing. The test is ordered infrequently, so the laboratory scientist consults the reference laboratory manual for instructions on how to process the sample. The processing instructions say to send two samples, a serum sample and a 1:100 dilution of serum, both refrigerated. 1. The sample received by the laboratory is in a gold-topped tube with a serum separator. Is this sample adequate to process for the send-out test? Select the best letter choice and give a brief explanation. A. Yes. The tube is adequate for a test requiring serum. B. No. This tube is not adequate for a test requiring serum. 2. The laboratory scientist confirms that the sample is adequate for processing. What is his next step? Choose the best letter answer and give a brief explanation. A. Putting the sample in the refrigerator B. Centrifuging the sample C. Starting to make the required dilution for the test D. Mixing the…
You are asked to make 1/2 dilution using 1.5mL of serum. How much dilution do you need to use?
At the station there is a 2 mL microcentrifuge tube containing sheep blood and 3 tubes containing the following solutions: 0 mM, 300 mM, 600 mM of sucrose. The tubes are randomly labelled A, B, C. 2 mL of each solution were transferred to a different microcentrifuge tube, 20 ul of blood were then added to each tube, and they were mixed well by inverting the tube for multiple times. They were observed and it was determined whether the solution in each tube was hypotonic (Yes or No) relative to the blood cells. In addition, the solutions were diluted with water to help you determine which solution is which sucrose concentration. What sucrose concentration corresponds with each tube? Tube Hypotonic (Yes/No) Hypotonic after 1:1 dilution with water (Yes/No) Most likely Sucrose concentration (mM) A No Yes B Yes Yes C No No
A 12-weeks pregnant woman complains of inability to focus, fatigue, and shortness of breath. On a physical examination, she looks pale and weak. Laboratory results are as follows: Reticulocyte count: 1%, Hematocrit: 27%; Hemoglobin: 6.5 g/dL; MCV: 105 fL, MCHC: 32%. Does this patient need a transfusion? Justify your answer. If the physician decides to give red blood cells to the patient, are there any special considerations given to the blood product? Justify your answer. If the patient's transfusion reactions history suggests anaphylactic reactions, which blood component should be given? Justify your answer.
What is the purpose of the RPR (RAPID PLASMA REAGIN ) Test? Why is a qualitative test performed before a semi-quantitative test? Why is it necessary to rotate the slide/card?
The laboratory requests of the physician are Glycosylated Hemoglobin and Serum Glucose for Mr. John Henry. B. After 5 minutes centrifugation the serum is pinkish to red in color. Is the specimen acceptable for the above-mentioned tests? Give a thorough explanation.
Give five (5) other examples of samples that are best prepared using Smear Preparation Technique (example: Human RBCs). Explain why.
The order is to give 45 mEq. The dosage available is 30 mEq in 15 mL. How many mL do you give?
Please explain: After making the 1:100 dilution, the laboratory scientist puts both the original serum sample and the labeled 1:100 dilution into the refrigerator. What is the appropriate temperature setting for the refrigerator? Choose the best letter answer and give a brief explanation. A. –10°C to – 15°C B. 1°C to 8°C C. 22°C to 24°C D. 37°C
You need to prepare medium for your culture cells. Your salt solution is supplied at a 10X concentration but needs to be 2X for use. You also need to add fetal bovine serum for a final concentration of 8%. What would you add of each for the correct final concentrations in 5 L of media?
Provide mock lab tests (blood, urine, etc.) and state the results. Also, add the normal range values.