5ml of 1 x 10° cells/ ml cell suspension is required to be prepared for seeding on the cell culture plate for the assay. How that cell suspension is prepared if the following data is given on the cell stock suspension? (6%) Hemocytometer No. of bright dots 1st large square 52 2nd large square 48 3rd large square 51 4th large square • Dilution factor: 5 49

Curren'S Math For Meds: Dosages & Sol
11th Edition
ISBN:9781305143531
Author:CURREN
Publisher:CURREN
Chapter10: Reconstitution Of Powdered Drugs
Section: Chapter Questions
Problem 7.8P
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5. LC50 of drug X acting on the fibroblast cells for 24hrs is going to be determined
by MTT assay.
a) 5ml of 1 x 10$ cells/ ml cell suspension is required to be prepared for seeding on
the cell culture plate for the assay. How that cell suspension is prepared if the
following data is given on the cell stock suspension? (6%)
Hemocytometer No. of bright dots
1st large square
52
2nd large square
48
3rd large square
51
4th large square
Dilution factor: 5
49
Transcribed Image Text:5. LC50 of drug X acting on the fibroblast cells for 24hrs is going to be determined by MTT assay. a) 5ml of 1 x 10$ cells/ ml cell suspension is required to be prepared for seeding on the cell culture plate for the assay. How that cell suspension is prepared if the following data is given on the cell stock suspension? (6%) Hemocytometer No. of bright dots 1st large square 52 2nd large square 48 3rd large square 51 4th large square Dilution factor: 5 49
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