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- Using G-25 Sephadex beads (Fractionation range 1000-5000) KD, a sample of Vitamin B12 (MW ~1500 KD) will be: Partially included Fully excluded Fully includedA member of the staff at the care home inquires about paracetamol with the pharmacist. This employee requested to purchase 4 boxes of paracetamol (each having 16 pills), but the grocer refused. What is the reason behind this? Please provide a biochemical explanation. please make the answer longDate Sheet Extraction ODD EVEN ODD EVEN GROUP Single Extraction 1 Single Extraction 2 Multiple Extraction 1 Multiple Extraction 2 Weight of tea leaves 10.002 (g) 10.123 (g) 10.013 (g) 10.007 (g) Weight of evaporating dish + caffeine 123.689 (g) 124.334 (g) 121.815 (g) 126.523 (g) Weight of empty evaporating dish 123.513 (g) 124.147 (g) 121.614 (g) 126.246 (g) Weight of caffeine Post Lab Questions Explain the principles involved in extraction. What other organic compounds can be extracted from tea bags? Draw their structures and briefly mention their biological function/s. Why do you need to add sodium carbonate in the extraction process?
- Sucrose + H2O(negative control): Colorless to brown solution: Blue solution Conclusion for Iodine test A. amylose present B. amylose not present Conclusion for Benedict's test A. reducing sugars not present B. reducing sugars present Sucrose + HCl(potential hydrolysis): unchanged compared to sucrose+water: Conclusion for Iodine test A. more amylose present B. LESS amylose present C. NO change Conclusion for Benedict's test a. more reducing sugar present b. less reducing sugar present c. no change Did hydrolysis of sucrose occur in the presence of acid? A. Yes B. noCyclophosphamide, after reconstitution, has a concentration of 20 mg/mL. The patient, who weighs 67 kg and is 155 cm tall, is ordered cyclophosphamide in 250 mL NS. How many mL will be added to the bag?Prepare a complete analysis procedure using KMnO4 as teh oxidant instead of K2CrO7 in the redox analysis of iron. Include sample and solution preparation, approximate weights of samples and reagents, procedure and chemical reactions. In particular, what is the purpose of the Zimmermann-Reinhardt reagent?
- Bial’s Orcinol Test Reagent’s Composition Observations: Test Sample Observations Water Glucose Fructose Arabinose Inferences: What is the principle behind this test? What kind of sugars will give a positive result to this test?You have a sample at 50 ng/ul and you would like to load 400ng of this sample on a lane of an agarose gel. You also have TE buffer as diluent and 6x loading dye. Your total sample should be 12ul. Calculate the amounts of each reagent necessary to prepare this sample for gel loading.Codeine 6 mg/ mL Simple syrup qs ad 2 floz (60 mL) a. Calculate the number of grams of codeine needed b. If we have codeine 30 mg tablets in stock, how many tablets are required? c. Calculate the percentage strength and ratio strength of this preparation 2. Acetazolamide Using Acetazolamide 250 mg tabs X 10 Simple syrup qs 100 mL a. Make qs 250 mL b. Find concentration