1. Shown below is the chromatogram for some amino acid standards and two unknowns. Each unknown consists of just a single amino acid. Answer the following questions based on this chromatogram. Solvent Frort Urkx Unk Y Ala Ser Asp a) Explain the trend in the distances travelled by the standard amino acids. b) Calculate the R;values of the standard amino acids and the two unknowns. Show your computations. c) Based on your calculations, what are X and Y? d) Are you able to identify X unambiguously? How about Y? e) If you are unable to identify either one of the unknown amino acids, think of a method by which you can separate them from each other. Explain your answer.

1. Shown below is the chromatogram for some amino acid standards and two unknowns. Each unknown consists of just a single amino acid. Answer the following questions based on this chromatogram. Solvent Frort Urkx Unk Y Ala Ser Asp a) Explain the trend in the distances travelled by the standard amino acids. b) Calculate the R;values of the standard amino acids and the two unknowns. Show your computations. c) Based on your calculations, what are X and Y? d) Are you able to identify X unambiguously? How about Y? e) If you are unable to identify either one of the unknown amino acids, think of a method by which you can separate them from each other. Explain your answer.

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

See similar textbooks

Similar questions

An amino acid mixture consisting of lysine,leucine, and glutamic acid is to be separated by ion-exchangechromatography, using a cation-exchange resin at pH 3.5, with theeluting buffer at the same pH. Which of these amino acids will beeluted from the column first? Will any other treatment be neededto elute one of these amino acids from the column?
Most protein dissolve in neutral salt solution, is that true? Provide reasoning
A mixture of proteins contains four different polypeptides, all in ~equal concentration, in solution with the following properties: Protein Molecular Mass (kDa) Isoelectric point A 45 4.5 B 77 6.0 C 28 4.1 D 14 10.7 A fraction of the protein solution is applied to a strong cation exchange column using a buffer at pH 8.0 with increasing [NaCl] from 0.05 M – 1.0 M. The chromatogram is shown below: 1. Based on the data presented, which of the following statements is true: Peak #1 is protein D Peak #4 is protein C Peak #3 is protein A Peak #4 is protein D Peak #2 is protein B 2. Since you know that the proteins are all present in approximately equal concentrations, the different relative peak areas tell you that: There are more neutral amino acids in protein #4…
Four known amino acids (AA) produce a chromatograph with Rf values as follows: AA1 =0.25, AA2 =0.66, AA3=0.50,AA4=0.80. A mixture of three amino acids yields spots that are 2 cm,5.2cm, and 6.4 cm from the organ. The solvent front is 8.0 cm from the origin. Which three of the four amino acids are contained in the mixture?
In what way can alkaloidal reagents precipitate proteins? In excess of alkaloidal reagent, did the precipitate formed dissolve or not? What is the evidence for your answer?
5 You need to separate the following three proteins, Protein A (MW 76 600 Da; pI 4.5); Protein B (MW 70 000 Da and pI 8.2) and Protein C (MW 42 000; pI 8.6). Describe what column/s you would use, the pH of the buffer/s you would use, which proteins are retained on the column and how you would elute the bound protein/s. How would you monitor where the protein peaks elute and whether the protein in each peak is pure of not?
If you added three proteins (Protein A, pI 5.0; Protein B, pI 6.0; Protein C, pI 7.0) to an anion exchange column equilibrated with bicine buffer, pH 8.5, in what order would these protein be expected to elute as the column was loaded, washed, and then increasing amounts of NaCl were added?
Table 2. Volume of BSA, protein content, and absorbance readings of reference solutions Solution Volume of BSA standard solution (μL) Protein content(μg/mL) Absorbance value At 595 nm 1 0 0 0 2 10 1 0.022 3 30 3 0.065 4 50 5 0.106 5 70 7 0.178 6 100 10 0.299 7 120 12 0.380 Make a graph by plotting the absorbance values versus the BSA protein content (in μg) for theseven reference solutions. When constructing the graph, be…
Five amino acids were subjected to normal phase chromatography, and the following Rf values were computed: A - 0.59 B - 0.12 C - 0.71 D - 0.33 E - 0.20 Which is the most polar amino acid?
Given a stock protein solution with a concentration of 6 mg/ml, determine the protein concentration of a solution made by mixing 5 μl of the stock with 5 μl of a buffer.
In an experiment, what happens to the calculated molar mass after waiting an hour between thawing the frozen cyclohexane and adding the unknown compound, during which time some of the cyclohexane evaporated. Will the molar mass be too large, too small, or unaffected? Explain.|
Which organic molecules may be found in urine from a patient with kidney illness, and which biochemical test would be the most suited for detecting those molecues?