1. The following reactions can be coupled in the citrate cycle. kJ/mol kJ/mol Succinyl-CoA GDP + Pi. succinate + CoA GTP G=-34.0 Gº= 30.5 a) Write the net reaction. Calculate Gº for the coupled reaction. Is the reaction favorable? b) Write the equilibrium constant in terms of reactant and product concentration. Calculate the equilibrium constant, K, for the coupled reaction at 37°C. c) If the ratio of [GTP/[GDP] in the cell is 22, the [CoA]/[succinyl-CoA] ratio is 0.20, and the [Pi] is 1.3mM, determine the [Succinate] at equilibrium. (Pay attention to concentration units)

1. The following reactions can be coupled in the citrate cycle. kJ/mol kJ/mol Succinyl-CoA GDP + Pi. succinate + CoA GTP G=-34.0 Gº= 30.5 a) Write the net reaction. Calculate Gº for the coupled reaction. Is the reaction favorable? b) Write the equilibrium constant in terms of reactant and product concentration. Calculate the equilibrium constant, K, for the coupled reaction at 37°C. c) If the ratio of [GTP/[GDP] in the cell is 22, the [CoA]/[succinyl-CoA] ratio is 0.20, and the [Pi] is 1.3mM, determine the [Succinate] at equilibrium. (Pay attention to concentration units)

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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