1. What is the N-terminal AA residue? 2. What is the C-terminal AA residue? 3. What is the sequence of Peptide 1?
Q: Draw the ff. peptides: DK YES CHEM What is the pI of each peptide? Predict the ionic form of the…
A: Peptides are small polymer of amino acids which is linked by peptide/amide (covalent bond). Alpha…
Q: I have four amino acids: serine, histidine, alanine, and tyrosine. How many different primary…
A: Proteins are composed of amino acids linked together by peptide linkages. T he primary structure of…
Q: what is the c-terminal AA residue? use one-letter code for amino acid
A: The amino acid can be defined as the organic compound that comprises an amino group at one end and a…
Q: 1. What is Amino Acid and its relation to proteins? 2. What is the importance of using multiple…
A: Since there are two questions asked, we will solve them both.
Q: When answering the questions below, please use the one-letter code for the amino acid, with NO…
A: Acid hydrolysis: Acid hydrolysis of any protein/polypeptide is being used to detect amino acids…
Q: Explain why is it necessary for a protein to adopt specific tertiary and quaternary arrangements.…
A: Proteins are defined as large biomolecules, or macromolecules, consisting of one or more long chains…
Q: Peptide #1: L-I-T-V Peptide #2: C-Q-H-R Peptide #3: E-G-E-A Which peptide is the most basic?…
A: Amino acids are the building blocks of protein. An amino acid contains a carboxylic acid and a-amino…
Q: 4. Draw the bond-line structure of the peptide that corresponds with the following sequence of amino…
A: Polypeptides are the long chain of the monomer units of amino acids, which are joined by peptide…
Q: 8. One amino acid contains a guanidino group. What is the approximate pka of the weak base in the…
A: Amino acids are organic compound having two functional group namely carboxyl and amino. An amino…
Q: Write the names of the appropriate regions on L-shaped structure of TRNAS. 13' 15'
A: The process of protein synthesis involves transcription and translation. In transcription, DNA is…
Q: How many peptide bonds are present in peptide 1? What is the N-terminal amino acid in peptide 2?…
A: Peptides are made up of amino acid joined together by peptide bond. Peptide bond is formed between…
Q: 1. Explain why the primary structure sequence -Lys-Leu-Trp-Asp- may promote a-helix formation while…
A: The formation of α helix is occurred due to attachment of H of N of Cα of first (n) amino acid to…
Q: Please describe the events that may result in a mature protein not having methionine as the…
A: Translation process of formation of a sequence of amino acid using messenger RNA as a template.
Q: 5' UGG CAA UCC UAC GAU 3' < Is it possible for a single base pair substitution to cause a truncation…
A: Yes, it is possible for a single base pair substitution to cause a truncation in the peptide as…
Q: what is the N and C terminal AA residue?
A: DNA sequencing is a biochemical method for determining the order of the nucleotide bases, adenine,…
Q: Some point mutations cause a change in amino acid sequence but nonetheless may have little to no…
A: option f is correct . This is because each group of amino acid belong to same category means . If…
Q: What is the net charge of the peptide at pH 7 that is synthesized from this DNA sequence? 5'…
A: As we know that the DNA sequence exhibits codons, which helps in the formation of mRNA, and then…
Q: Are they acidic, basic, or neutral?
A: pH is the negative logarithm of hydrogen ions in a particular same. The lower the pH higher will be…
Q: Draw a peptide bond between Phenylalanine (c-terminus) and Isoleucine (n-terminus).
A: Amino acids are organic compounds with two functional group namely carboxyl and amino. There are 20…
Q: 1. Given the anticodon in tRNA is CUU, name the amino acid that will be added. 2. Given the…
A: Since it is a multipart question we are supposed to answer only the first three questions as per the…
Q: what is peptide linkage?how is tripepide formed ?
A: Proteins are the building blocks of the body. They are organic molecules composed of amino acids.…
Q: Does right-handed alpha-helix has the least restricted ϕ and ψ angles in polypeptides, compared with…
A: When amino acids are joined with one another, the C-N bond in the amide linkage causes rigidity…
Q: N' C' = Polypeptide X d a b = SRP 5'- = translocator e Which region(s) of polypeptide X would bind…
A: Ribosomes refer to the site where the protein synthesis takes place. It comprises rRNA and protein.…
Q: 1. What is the N-terminal AA residue? 2. What is the C-terminal AA residue? 3. What is the sequence…
A: Aminoacids are organic compunds. that combine to form protein. Amino acid is formed when a central…
Q: PIKE What is its charge at pH8? How many buffering regions does it have and write these regions down…
A: The PIKE peptide is composed of amino acids. The amino acids which make PIKE are isoleucine,…
Q: Given the polypeptide chain below: Alanine - Arginine - Valine - Histidine - Aspartic acid -…
A: Amino acids are organic molecules comprised of two functional groups that are an amino group and the…
Q: Calculate the pI of both peptides. Which is more acidic and why?
A: Given sequence, PEPTIDE #1: Pro-Phe-Thr-Cys PEPTIDE #2: Gly-Tyr-Ser-Asp If the peptide does not have…
Q: 1. How many different tripeptides are possible? How many carboxyl terminals of polypeptide chains…
A: Tripeptides are composed of three amino acids. There are 20 naturally occurring amino acids . A…
Q: You are working in a bioengineering lab, and you are asked to create a peptide with a helix with…
A: Proteins are the macromolecules composed of amino acids bound together by peptide bond between amino…
Q: what is the correct way of drawing a peptide backbone?
A: A peptide is a compound consisting of two or more amino acids.
Q: In relation to the peptide sequence that is presented. -Gly - Ser – Cys – Asp – Glu – Arg – Cys –…
A: The given peptide sequence contains 8 amino acids. In a peptide, individual amino acids are joined…
Q: O The unknown sample contains tyrosine and tryptophan residues O The unknown sample only contains…
A: Enzymes are biocatalyst that increases the speed of reaction by lowering the activation…
Q: A peptide digested with trypsin produced the following three fragments: C, ASFPK, GGRWDGK The same…
A: Proteolysis or protein digestion is the process of breaking down of proteins using enzymes known as…
Q: How many amino acids long is a zinc finger domain? What four conserved residues are contained in the…
A: Zinc finger are structural protein motif that has Zn ion in a coordinated bond to form a stable…
Q: Determine the identity of the N-terminal amino acid after reconstructing the intact protein. Why is…
A: Introduction:- Amino acids are the building blocks of proteins. The building blocks of life are…
Q: How can we order the peptides to obtain the primary structure of the original protein?
A: Primary structure of protein - These are linear sequence of amino acid that forms the backbone of…
Q: what is the n-terminal AA residue? use one-letter code for amino acid
A: The N-terminal amino acid residue is the amino acid that is present in the N-terminal. For protein…
Q: What are congenital disorders of glycosylation (CDGs)?
A: Congenital disorders of glycosylation is a broad term used for rare genetic and metabolic disorders…
Q: Which peptide would be more soluble at pH 7.0, (Val)₂₀ or (Asp)₂₀ ? at pH 3.0, (Gly-Glu-Val)
A: Proteins have their least solubility close to the pH that is equal to their pI.
Q: When answering the questions below, please use the one-letter code for the amino acid, with NO…
A: Protein sequencing is the process of determination of amino acid sequence in a protein. During the…
Q: What is the significance of the positioning of the amino acid side chains and alpha carbons as you…
A: Proteins are polymers of amino acids linked by peptide bond/amide bond. Peptide bond is a covalent,…
Q: 1. What nitrogenous base is found in position Y? 2. What nitrogenous base is found in position X? 3.…
A: Nucleotides are the building blocks of DNA and RNA. The process of transcription produces messenger…
Q: B. Draw the structure of the given peptides. 1. Ser-Asp
A: Peptides are composed of standard amino acids. There are 20 standard amino acids that makeup all the…
Q: Which of these peptides is the most hydrophobic? A. KFYV B. ERSC C. PIMF
A: Proteins are modified polypeptide chains. These chains are made up of 10 or more amino acids linked…
Q: Which of the following peptides will likely adopt an alpha helix? H-I-R-E-F A-D-L-E-E A-D-E-L-E…
A: The most common types of secondary structure are alpha-helix and beta-pleated sheets. The…
Q: 1. You are working in a bioengineering lab, and you are asked to create a peptide with a helix with…
A: The arrangement of amino acids in polypeptide such that it gives rise to recurring structural…
Q: 1) what is the net charge of the amino acid leucine of it is on the C-terminus end and why? 2) if…
A: Proteins are among the most numerous organic molecules in biological systems, and their structure…
Q: 2. Of the two sequences, which is more likely to have helical structure. Explain. a)…
A: The amino acids are the protein structure alphabet; they can be arranged to create an almost…
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- Draw the structure of the PTH derivative product you would obtain by Edman degradation of the following peptides: (a) I-L-P-F (b) D-T-S-G-Ayou have isolated the following peptide: His-Ser-Arg- Ala-Glu- Leu- Pro- Gly Calculate the approximate charge of the peptide at Ph 1, 3, 5, 8, 11, 14 AND what is the PI of this peptide?A peptide composed of 40 amino acids (aa) was isolated from the venom of the Gila Monster(Heloderma suspectum, then submitted to various biochemical and chemical treatments.1) After a treatment with a highly specific chymotrypsin, four peptides were generated:- peptide A (6 aa)- peptide B (16 aa)- peptide C (3 aa)- , and peptide D (15 aa)2) Treatment with carboxypeptidases A or B resulted in the release of:- phenylalanine for peptides A and B- tryptophan for peptide C- glycine for peptide D and the 40 amino acids venom peptide3) One cycle of Edman degradation either for peptide A or for the 40 amino acidspeptide gave the exact PTH derivative indicated below: 4) The 40 amino acids peptide treated with Asp-N endopeptidase followed by massspectrometry MS/MS revealed the following composition:- two molecules of glutamic acid,- various peptides indicated below sorted by masses:HG,DLSKQM,EGTFTS,EAVRLFI,EWLKNGGPSSGAPPPSG Information- Chymotrypsin (endopeptidase) hydrolyzes peptide bonds on…
- The oligopeptide isolated from the brain of an animal has the sequence glu-his-tri-ser-tyr-gly-leu-arg-pro-gly. Determine the total charge of the molecule at pH 3.0; 5.5; 11.0. In which pH range there is the isoelectric point of the peptide?You have isolated the following peptide: His-Ser-Arg-Ala-Glu-Leu-Pro-Gly A) Calculate the approximate charge of this peptide at pH 1, pH 3, pH 5, pH8, pH 11, and pH 14. B) What is the PI of this peptide?For the following pentapeptides: Ser-Glu-Gly-His-Ala and Gly-His-Ala-Glu-Ser Draw the most protonated forms of the pentapeptides Illustrate the ionization of the pentapeptides from the most protonated to its deprotonated form. Label the ionization with its respective pK values. Compute their isoelectric pH (pI). Do these peptides with the same amino acid composition have different net charges at pH 7.0? Explain briefly. Would you expect the titration curves of the two peptides to differ? Why or Why not?
- VII. Analysis of a peptide antibiotic purified from a strain of Bacillus brevii resulted in the following significant information: Complete hydrolysis of the peptide yielded equimolar amounts of Leu, Orn, Phe, Pro, and Val Molecular weight of the peptide was estimated to be aroung 1,200 Da The peptide failed to undergo hydrolysis when treated with carboxypeptidase Partial hydrolysis and then chromatographic separation of products yielded the following di- and tripeptides: Leu-Phe, Phe-Pro, Orn-Leu, Val-Orn, Val-Orn-Leu, Phe-Pro-Val, Pro-Val-Orn Treament with dinitrofluorobenzene (DNFB) followed by complete hydrolysis yielded only free amino acids and the DNFB-derivatized ornithine at its side chain Using this set of information available to you, deduce the amino acid sequence of this peptide antibiotic. Write your final peptide sequence only using 3-letter abbreviations.For the following pentapeptides: Ser-Glu-Gly-His-Ala and Gly-His-Ala-Glu-Ser A. Compute their isoelectric pH (pI). Show full solution. Use standard pKa values. B. Do these peptides with the same amino acid composition have different net charges at pH 7.0? Explain briefly. C. Would you expect the titration curves of the two peptides to differ? Why or Why not?You have isolated from a rare fungus an octapeptide that prevents baldness and you wish to determine its amino acid sequence. The amino acid composition is K2, D, Y, F, G, S, A. Reaction of the intact peptide with dansyl chloride yields dansyl-A. Cleavage with trypsin yields peptides whose compositions are: (K, A, S) and (G, F, K) plus a dipeptide. Reaction with chymotrypsin releases free D, a tetrapeptide with composition (k, S, F, A) and a tripeptide whose composition following acid hydrolysis is (G, K, Y). The enzymatic digests are each carried out on the whole, undansylated peptide.What is the sequence?
- Write the structure of the following peptides: 1. glycyl alanine 2. tyrosyl alanyl glycine 3. phenylalanyl tyrosyl seryl histidine 4. valyl glycyl cystein 5. seryl methionineyou have the following peptide Arg-Ile-Pro-Leu-Asp-Lys-Glu The net charge on this peptide at pH 7.0 is ?. The net charge on this peptide at 1M HCl is ?.Treatment of a polypeptide with 2-mercaptoethanol yields two polypeptides 1. Ile—Val—Cys—Arg—Thr—Gly—Cys—Lys—Asn—Phe—Leu2. Trp—Lys—Cys—Phe—Arg—His—Thr—Lys—Cys—SerTreatment of the intact polypeptide with trypsin yields fragments with the following aminoacid compositions(Arg, Cys2, Ile, Ser, Val)(Arg, Cys2, Gly, Lys, Thr, Phe)(Asn, Leu, Phe)(His, Lys, Thr)(Lys, Trp)Edmands chemistry reveal the following PTH derivatives in equal quantity after cycle 1• PTH--Ile and PTH--Trp a) Rewrite the intact polypeptide, by indicating the positions of the disulfide bonds(Indicate your reasoning) b) How many products do you expect after chymotrypsin cleavage of the intactpolypeptide? c) You are performing an isoelectric focusing experiment (IEF) on a gel with a pH range of 2to 14. Indicate on the diagram below at what point along the gel will peptide 1 abovefocus, in the presence (X) and absence (Y) of DTT. (also show your calculations)