1. You have a culture of yeast that is at a density of 8x107 cells/mL2. A lab technician took a fecal sample andcarried out the following dilutions:1. Transferred 0.1 mL from the fecal sample0.1 mlI ml0.01 mlto 9.9 mL saline in tube A2. Transferred 1 mL from A to 9 mL salinein tube B3. Transferred 0.01 mL from B to 9.99 mL0.1 mlACsaline in tube C4. Transferred 0.1 mL from tube C to an agarplate5. Counted 65 colonies on plate D9.9 ml9 ml9.99 mlsalinesalinesalineD5.What is the concentration of cells in tube C?6. What is the concentration of cells in the fecal sample?1

Serial dilution is a process of diluting the stock sample to decrease its concentration by serially…

1. You have a culture of yeast that is at a density of 8x10' cells/mL 2. A lab technician took a fecal sample and carried out the following dilutions: 1. Transferred 0.1 mL from the fecal sample 0.1 ml 1 ml 0.01 ml to 9.9 mL saline in tube A 2. Transferred 1 mL from A to 9 mL saline in tube B 0.1 ml 3. Transferred 0.01 mL from B to 9.99 mL A C saline in tube C 4. Transferred 0.1 mL from tube C to an agar plate 5. Counted 65 colonies on plate D 9.9 ml 9 ml 9.99 ml saline saline saline D 5. What is the concentration of cells in tube C? 6. What is the concentration of cells in the fecal sample?

1. You have a culture of yeast that is at a density of 8x10' cells/mL 2. A lab technician took a fecal sample and carried out the following dilutions: 1. Transferred 0.1 mL from the fecal sample 0.1 ml 1 ml 0.01 ml to 9.9 mL saline in tube A 2. Transferred 1 mL from A to 9 mL saline in tube B 0.1 ml 3. Transferred 0.01 mL from B to 9.99 mL A C saline in tube C 4. Transferred 0.1 mL from tube C to an agar plate 5. Counted 65 colonies on plate D 9.9 ml 9 ml 9.99 ml saline saline saline D 5. What is the concentration of cells in tube C? 6. What is the concentration of cells in the fecal sample?

An Illustrated Guide To Vet Med Term

4th Edition

ISBN:9781305465763

Author:ROMICH

Publisher:ROMICH

Chapter11: The Great Communicator

Section: Chapter Questions

Problem 14MC

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1. You have a culture of yeast that is at a density of 8x107 cells/mL 2. A lab technician took a fecal sample and carried out the following dilutions: 1. Transferred0.1 mL from the fecal sample 0.1 ml 1 ml 0.01 ml to 9.9 mL saline in tube A 2. Transferred 1 mL from A to 9 mL saline in tube B 3. Transferred 0.01 mL from B to 9.99 mL saline in tube C 4. Transferred 0.1 mL from tube C to an agar plate 5. Counted 65 colonies on plate D 0.1 ml B 9 ml saline 9.9 ml 9.99 ml D saline saline Questions: 1. What is the serial dilution in A? 2. What is the serial dilution in B? 3. What is the serial dilution in C? 4. What is the total dilution in C? 5. What is the concentration of cells in tube C? 6. What is the concentration of cells in the fecal sample? 1
4. Answer the following questions about dilutions. b. C You take a culture of bacteria and dilute it 5 times. Each dilution uses 1 ml of culture and 9 ml of saline solution. You take.Iml from the most dilute tube and plate it on a TSA plate. The next day you count 61 colonies. What was the original concentration 6.1X10³ CFU/ ON 6.1X 10² CFU Od 61 X 10² CFU/ml 61X10 CFU/ You have a culture that has 5x10 cfu/ml of bacteria in it. You performa serial dilution by adding 1 mi of the stock in 9ml of TSB inTubeA and mix You then take 1 ml from Tube A and add it to 9 ml of TSB in Tube Band mix Last you take 1 ml from tube 8 and add it to a TSA plate. After overnight incubation, you count the colonies you see. How many colonies will you see? 5000 N 500 Od 50 Oas Oes You are doing a series of serial dilutions to find the concentration of bacteria in CFU/ml in a culture. You plate several of the dilutions tubes and count the colonies the next day. On plate A you find over 1000 colonies, plate B…
1. You wish to determine viable counts on a culture of Bacillus subtilis. You begin by pipetting 1 ml of culture into 99 ml of sterile water. After mixing the dilution well you make a series of 4 further dilutions of 10-1 each. From the three most dilute samples, you prepare three pour plates using 1 ml in each. After incubation you find the plate counts of the plates are 16, 245 and 890 respectively. a) Show the dilution scheme. b) What is the estimated viable count (cells/ml) in the original culture? 2. Scientific Notation. Fill in the missing information. a) 4.5 x 109 = _______ b) 50 x 107 = _______ c) 2300 x 1010 = _______ d) 0.54 x 108 = _______
Refer to the following diagram: 100 ul 100 uL 100 pl 100 µL 100 pl 100 pL 100 pl Volume to transfer A B C D E F G 900 uL in each tube 10 102 10 10 10 10 10 Dilutions E.CoN culture Volume of the diluted sample plated 100 ul 100 μL 100 pL 100 ul Volume of the 10 ml 10 ml original sample plated 10 ml 10 ml Plate D Plate E Plate F Plate G Given that Plate D has too many colonies to count, and Plate G has no colonies at all as a result of serial dilution, draw out all four plates and demonstrate what they might look like (assuming serial dilution has been performed correctly). Based on your drawings, identify the number of CFU/ml for Plate E and Plate F. Show your calculations.
2. Determine the number of bacterial cells per ml. in the original culture. Iml 1ml Iml 1ml 99ml 99ml 99ml 99ml oliginal pecimen E. col 1ml 10.1ml 1ml 0.1ml 1nml 0.1ml too many to count >500 128 12 0 colony counts
Below is a diagram of the serial dilution of a culture with 1 x 10º cells. Fill out the missing information. 250 Culture Diluent 1 x 10° cells/mL 9 mL 9 mL 9.9 mL 9.9 mL 99.9 mL Volume to add to diluent Final Dilution level Theoretical count after plating 100 uL
6. Answer the following questions about colony forming units. a. b. You take a culture of bacteria and dilute it 5 times. Each dilution uses 1 ml of culture and 9 ml of saline solution. You take .1ml from the most dilute tube and plate it on a TSA plate. The next day you count 61 colonies. What was the original concentration a) 6.1 X 105 CFU/ml b) 6.1 X 105 CFU/ml c) 6.1 X 108 CFU/ml d) 6.1 X 107 CFU/ml You are doing a series of serial dilutions to find the concentration of bacteria in CFU/ml in a culture. You plate several of the dilutions tubes and count the colonies the next day. On plate A you find over 1000 colonies, plate B you find 157 colonies and plate C you find 7 colonies. Which plate(s) can be used to determine the concentration in the original tube? a) Plate A Ob) None of the plates can be used c) Plate C d) Plate B e) Plates A, B and C
Volume of culture Start transferred for ** time serial dilution A B Volume of sterile 900 µL 900 µL 900 µL 900 µL water added to each tube prior to the transfer of bacteria Bacterial 9.9 mL culture broth at °C Volume of the diluted sample plated 20 µL 20 µL 20 µL 20 µL Plate A Plate B Plate C Plate D **Repeat this transfer at various time points into a new set of tubes
3. A bacterial culture was diluted and results from duplicate plates were obtained as indicated below. What was the number of colony forming units/mL of the original culture? Dilution used for plating 10-2 10-3 104 10-5 10⁰ 10-7 10-8 4. 5. Amount plated 0.1 mL 0.1 mL 0.1 mL 0.1 mL 0.1 mL 0.1 mL 0.1 mL Colony counts after incubation (Results from duplicate plates) Too many to count Too many to count 341 413 99 175 27 : 29 7:2 0:0 Ten grams of hamburger were added to 90 mL of sterile buffer. This was mixed well in a blender. One-tenth of a mL of this slurry was added to 9.9 mL of sterile buffer. After thorough mixing, this suspension was further diluted by successive 1/100 and 1/10 dilutions. One-tenth of a mL of this final solution was plated onto Plate Count agar. After incubation 145 colonies were present. How many colony-forming units were present in the total 10 gram sample of hamburger? Devise a serial dilution scheme to prepare a 10-5 dilution on a plate using the least number of…
Young woman applies to her health center with burning and frequent vomiting but has no fever. The doctor diagnoses acute cystitis and the patient is allowed to leave a urine sample. a. How should the urine sample be taken? And why is this technology chosen? b. A rapid test is performed on the urine sample via a test strip that has reagent fields for nitrite. After 1 min read the strip which is pink colored. Which bacterial species is most likely in the urine sample? c. What is the reservoir for this urinary tract pathogen?
You are working in a medical lab and receive a urine sample from a patient that is displaying urinary tract infection symptoms. You use the urine to set up 8 serial 10-fold dilutions. You decide to spread plate 100 µl of different dilutions. The colony count after incubation overnight: 10-4 dilution has 173 colonies. What is the cfu/ml of this culture?
This agar plate was obtained by spreading 0.1 ml of a 1:1,000 dilution of a bacterial sample taken from cole slaw, and then incubating at 37oC for 24h. This plate indicates that: a. there are 42 bacteria/ml on the cole slaw b. there are at least 4 different types of bacteria in the cole slaw c. there are approximately 42,000 bacteria/ml in the cole slaw sample d. there are approximately 420,000 bacteria/ml in the cole slaw e. these bacteria cannot cause illness because they came from the person’s skin who made the cole slaw